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제주 연안의 해수로부터 분리한 Cellulase 생산균 Bacillus sp. GC-1과 GC-4의 동정
지원재 ( Won Jae Chi ),박다연 ( Da Yeon Park ),( Uyangaa Temuujin ),이종열 ( Jong Yeol Lee ),장용근 ( Yong Keun Chang ),홍순광 ( Soon Kwang Hong ) 한국미생물생명공학회(구 한국산업미생물학회) 2011 한국미생물·생명공학회지 Vol.39 No.2
GC-1과 GC-4로 명명된 두 종의 그람 양성 박테리아가 제주도 연안해수로부터 동정되었다. 이 두 균주는 16S rRNA 유전자 염기서열 분석과 생리적 특성 분석결과를 토대로 Bacillus 속의 박테리아로 규명되었다. 균주 GC-1의 16S rRNA 유전자 염기서열은 B. tequiliensis와 B. subtilis subsp. inaquosorum의 16S rRNA 유전자 염기서열과 99.91%의 상동성을 보였고, 균주 GC-4의 16S rRNA 유전자 염기서열은 B. altitudinis, B. stratosphericus 및 B.aerophilus의 16S rRNA 유전자 염기서열과 100%의 상동성을 보였다. 그러나 두 균주의 생리학적-유전학적 특성 분석결과, 이들이 계통적 유연관계를 갖는 다른 Bacillus 속의 균주들과 상당한 차이가 있었고, 따라서 조사된 Bacillus 속과는 다른 속에 속할 가능성이 높았다. 이러한 결과는 Bacillus 속이 진화과정 중에 다양한 변종으로 진화되었음을 암시한다. Two Gram positive bacterial strains, designated strain GC-1 and GC-4, were isolated from coastal seawater near Jeju Island in the Republic of Korea. The two strains were identified as members of the genus Bacillus, based on 16S rRNA gene sequencing and data for physiological characteristics analyses. A subtle difference in physiological and genotypical characteristics has led us to designate the strains GC-1 and GC-4. The strain GC-1 showed a 99.91% similarity in 16S rRNA gene sequencing with B. tequiliensis and B. subtilis subsp. inaquosorum and the strain GC-4 showed a 100% similarity in 16S rRNA gene sequencing with those of B. altitudinis, B. stratosphericus, and B. aerophilus. However, both strains exhibited different physiological and genotypical characteristics in many aspects from those of their phylogenetically closest neighbors listed above, which implies that genus Bacillus has diversified into various species during its evolutionary process.
Xylanase 생산균 Bacillus sp. MX47의 분리 및 동정
지원재 ( Won Jae Chi ),박다연 ( Da Yeon Park ),박재선 ( Jae Seon Park ),홍순광 ( Soon Kwang Hong ) 한국미생물생명공학회(구 한국산업미생물학회) 2012 한국미생물·생명공학회지 Vol.40 No.4
A xylanolytic bacterial strain, MX47, was isolated from rotting plant matter in soil. The strain was aerobic and gram positive, and grew between pH 6.0 and 11.0. Cells were susceptible to thiostrepton and chloramphenicol. The major fatty acids (>3%) comprised 64.55% of iso-C15:0, 22.76% of anteiso-C15:0, and 3.92% of iso-C17:0. The G/C content of the DNA was 44.15 mol%. The predominant respiratory quinone was menaquinone 7 (MK-7). Searches for 16S rRNA gene sequence similarity as well as phylogenetic analyses strongly suggested that the strain should be classified to the genus Bacillus. However, its biochemical characteristics, including acid production and enzyme activities, are different from those of other Bacillus strains in the same clade, and therefore, we propose the name Bacillus sp. MX47.
유전자조작, 균주분리 대한민국 대천 해안에서 분리한 전분 분해능을 갖는 Pseudoalteromonas sp. A-3 균주의 특징 및 동정
지원재 ( Won Jae Chi ),박다연 ( Da Yeon Park ),정성철 ( Sung Cheol Jeong ),장용근 ( Yong Keun Chang ),홍순광 ( Soon Kwang Hong ) 한국미생물생명공학회 2011 한국미생물·생명공학회지 Vol.39 No.4
Strain A-3, an amylase-producing bacteria, was isolated from coastal seawater near Daecheon in the Republic of Korea. It was seen to possess a single polar flagella and grow well, on ASW-YP agar plates, at temperatures of between 20-37℃. However, it grew more slowly at the temperatures of 15℃ and 40℃. Similarly, it was observed to grow abundantly, in an Artificial Sea Water-Yeast extract-Peptone (ASW-YP) liquid medium, in a pH range of 6-9, but not grow at pHs of 4-5 and a pH of 10. Strain A-3 was noted as being close to Pseudoalteromonas phenolica O-BC30T, Pseudoalteromonas luteoviolacea NCIMB1893T, Pseudoalteromonas rubra ATCC29570T, and Pseudoalteromonas byunsanensis FR1199T, with 98.30%, 97.86%, 97.78%, and 97.25% similarities respectively, in its 16S rRNA sequence. A phylogenetic tree revealed that strain A-3 and P. phenolica O-BC30T belong to a clade. However, strain A-3 differed from P. phenolica O-BC30T in relation to a number of physiological characteristics. Strain A-3 exhibited no growth above 5% NaCl concentrations, no utilization of D-glucose, D-mannose, D-maltose, or D-melibose, and no lipase (C-14) activity. All of these properties strongly indicate that strain A-3 is distant from P. phenolica O-BC30T and thus led us to name it Pseudoalteromonas sp. A-3. Pseudoalteromonas sp. A-3 produces α-amylase throughout growth. Maximal amylase activities of 144.48 U/mL and 149.20 U/mL were seen at pH 7.0 and 37℃, respectively. Pseudoalteromonas sp. A-3`s high, stable production of α-amylase in addition to its biochemical features, such as alkalitolerance, suggest that it is a good candidate for industrial applications.
홍조류로부터 신규 한천분해미생물 Alteromonas macleodii subsp. GNUM08120의 분리 및 동정
지원재 ( Won Jae Chi ),임주현 ( Ju Hyeon Lim ),박다연 ( Da Yeon Park ),김무찬 ( Mu Chan Kim ),김창준 ( Chang Joon Kim ),장용근 ( Yong Keun Chang ),홍순광 ( Soon Kwang Hong ) 한국미생물생명공학회(구 한국산업미생물학회) 2013 한국미생물·생명공학회지 Vol.41 No.1
An agar-hydrolyzing marine bacterium, strain GNUM08120, was isolated from Sargassum fulvellum collected from Yeongil bay of East Sea of Korea. The isolate was Gram-negative, aerobic, motile with single polar flagellum, and grew at 1-10% NaCl, pH 5.0-8.0, and 15-37oC. G+C content and the predominant respiratory quinone were 46.13 mol% and Q-8, respectively. The major cellular fatty acids were Summed feature 3 (24.5%), C16:0 (21.7%), and C18:1ω7c (12.5%). Based on 16S rRNA gene sequence similarity and DNA-DNA hybridization analyses, strain GNUM08120 was identified as a novel subspecies of Alteromonas macleodii, designated Alteromonas macleodii subsp. GNUM08120. Production of agarase by strain GNUM08120 was likely repressed by the effect of carbon catabolite repression caused by glucose. The crude agarase prepared from 12 h culture broth of strain GNUM08120 exhibited an optimum pH and temperature for agarase activity at 7.0 and 40oC, respectively. The crude enzyme produced (neo)agarobiose, (neo)agarotetraose, and (neo)agarohexaose as the hydrolyzed product of agarose.