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항균성 Ag-30CaO·70SiO<sub>2</sub> Gel의 MC3T3 세포적합성에 관한 연구
윤금재,류재경,안응모,김윤종,김택남,노인섭,조성백,Yoon, Geum-Jae,Ryu, Jae-Kyung,An, Eung-Mo,Kim, Yun-Jong,Kim, Taik-Nam,Noh, In-Sup,Cho, Sung-Beck 한국재료학회 2014 한국재료학회지 Vol.24 No.12
It is known that bones get damaged by accidents and aging. Since the discovery of Bioglass, various kinds of ceramics have been also found to bond to living bone; some of these ceramics are already being clinically used as bone-repairing materials. In the present study, antibacterial calcium silicate gel ($Ag-30CaO{\cdot}70SiO_2$ gel) was prepared by sol-gel method in order to control the microstructure, which is related to the dissolution rate and induction period of apatite formation in body environment. In addition, biological $Ag-30CaO{\cdot}70SiO_2$ is tested. This was done to impart antimicrobial activity to the $30CaO{\cdot}70SiO_2$. Ag ion was added during sol-gel synthesis to replace the $H_2O$ added during the making of the $30CaO{\cdot}70SiO_2$ gel, which has silver solutions of various concentration. After the sol-gel process, 1N-$HNO_3$ solution was used to wash the gel when synthesizing the gel, in order to maintain the porous structure and remove PEG, water soluble polymers. Then, the apatite forming ability of the sol-gel derived CaO-$SiO_2$ gels was investigated using simulated body fluid (SBF), which had almost the same ion concentration as that of human blood plasma. The gels were analyzed by FT-IR spectroscopy, SEM observation, XRD, and fluorescent microscopy. The apatite was successfully created even after washing the gel; apatite is present in an amorphous state, and was found to affect the concentration of the Ag ion in cells in MC3T3 live & dead assay results. From these results, it is suggested that a good material that can be used to repair defects of nature bone is $Ag-30CaO{\cdot}70SiO_2$ gel.
최윤정 ( Yoon Jeong Choi ),문송현 ( Song Hyun Moon ),김미숙 ( Mi Sook Kim ),박다현 ( Da Hyun Park ),노인섭 ( In Sup Noh ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.1
We regenerated a vascular media tissue by seeding smooth muscle cells on the inside of a hybrid vascular scaffold. The hybrid vascular scaffold consisted of two porous biodegradable poly(lactide-co-glycolide) (PLGA) thin layers on both the inner and the outer surfaces of the non-biodegradable expanded polytetrafluoroethylene (ePTFE) layer. The PLGA layers were designed to have porous structures for control of tissue regeneration by impregnating and then gas-foaming of ammonium bicarbonates as porogens of the biodegradable layers. To improve tissue regeneration, we coated gelatin polymers on inside the porous biodegradable PLGA surface. Media tissue regeneration was successfully achieved by replacing the PLGA layers and the tissues were observed by H&E staining and Sircol collagen assay. Tissue formation was dependent on cell culture period. While the two PLGA layers were expected to serve as biological layers of intima, media and adventitia of the blood vessel after their transformation into the engineered tissues, the non-biodegradable ePTFE in the middle of the hybrid scaffold did as a mechanically supporting layer.
단백질 용액을 흡착시킨 고분자 표면상의 혈소판 상호작용에 관한 연구
노인섭 서울産業大學校 2000 논문집 Vol.51 No.2
Platelet adhesion onto various polymeric materials after protein adsorption has been tested in vitro by employing short-term measuring equipment such as in situ epifluorescence video microscopy. The adsorption of fibrinogen from 1% dilute plasma was performed on the surfaces of poly(urethane) derivatives films such as Biomer, poly(ether urethane urea), poly(ether urethane), silicone urethane copolymer silicone rubber and poly(ether urethane) derived with the anti-calcifying agent hydroxyethane bisphosphate. The polymer surfaces pre-adsorbed with fibrinogen were exposed in vitro to the heparinized human whole blood for the initial interactions of platelets and polymorphonuclear leuckocytes for 5 to 10 min. Dependence of finbrinogen pre-adsorption on the platelets adhesion onto the polymeric surfaces was evident from the measurement of adhered platelets and leuckocytes coverage area, i.e. the fibrinogen adsorbed polymers showed more cell coverage by cellular adhesion and spread than the control untreated polymers did. These results indicate that the adsorption of cell adhesion-inducing proteins is important for the designs of blood-contacting materials.