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금강유역에서 채집된 Lymnaea pervia, Lymnaeidae (애기물달팽이)외 3종(種)에 대한 계통유연성
나영언 建陽大學校 1998 建陽論叢 Vol.- No.6
RAPD(Randomly Amplified Polymorphic DNA) method was used to find out the phylogenetic relationships and the genetic polymorphisms on 4 species, C. chinensis, H. cantori, P. acuta and L. pervia collected in Keum-River basins. It was awared that 4 random primers among the used 20 primers were available to characterize the genetic relationships of 4 species freshwater gastropods. The data obtained from the above experiments were analyzed by PAUP Genetic Similarity Index Program. According to these analysis, the genetic similarity distance index was ranged from 0.5 to 0.9; that is it was revealed that C. chinensis was closely related to P. acuta and H. cantori to L. pervia as being treated to the specific primer sequences. From these results, RAPD analysis method could be succesively applied to the classification and genetic analysis for the phylogenetic relationships of the freshwater gastropods.
辛大煥,羅榮彦,權斗星 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1
Prevalence of the plerocercoid larva in the fresh-water fishes in Keum-river basin was surveyed from April to September, 1986. To observe whether this plerocercoid larva is really sparganum or not, and can be transfered to the paratenic host or not, a study was performed with the adequate experimental animal (domestic fowl and cat). It was collected the 1096 fresh-water fishes that was ranged 8.0^-16.0cm in length. The results obtained were as follows; 1. A number of ova of Diphyllobothrium spp, was confirmed under the microscopic study, 5 days after experimental infection through the mouth, and them sacrificed to the experimental animal (final host, cat). The adult worm of Diphylobothrium spp., 9.5cm in length, was obtained and that worm was located in the upper position of rectum, Therefore, this plerocercoid larva was confirmed as Sparganum. 2. Transference to the paratenic host (dowestic fowl) was not resulted. 3. Among the total number of 1096 fresh-water fishes, only the Z. platypus was infected with 107 worms corresonding to 9.8%. 4. Infection rate according to the length of Z. platypus was 66. 4% at 12-13. 9cm in length, and this percentage was the highest prevalent rate.
外來患者 및 돼지에서의 間接Latex凝集反應法에 依한 Toxoplasma 抗體價 調査硏究
심희숙,나영언,이영하,신대환 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2
This study was undertaken to konw the sero-epidemiologic meanings of toxoplasmosis by Indirect Latex Agglutination test of 514 general outpatients and 100 pigs. The results were obtained as follows: 1. Toxoplasma antibody titers were showed as 418 negative cases(81.3%), 63 cases(12.2%) in a titer of 1:16, 20 cases(3.9%) in a titer of 1:32, 8 cases(1.6%) in a titer of 1:64, 3 cases(0.6% ) in a titer of 1:128 and 2 cases(0.4%) in a titer of 1:256, respectively. 2. Antibody titer was somewhat declined to the nineteen ages (from 6.1% to 3,0%) but was increased from twenty to fifty-nine ages(from 9.1% to 24.2%). The positive percentage of Toxoplasma antibody titers were the highest as 24.2% in the fifties group. Totally, 33 cases(6.5%) among 514 patients was showed as positive reaction. 3. According to the sex group, 16 cases of the male and 17 cases of the female were showed as positive reaction 5.6%, 7.5%, respectively. Positive rates of the female was somewhat higher than that of the male. 4. Antibody titers of sera of the pig were shown as 65 negative cases(65%), 24 cases in a titer of 1:16(24%), 6 casesin 1:32(6%), 3 cases in 1:64(3%) and 2 cases in 1:1.28(2%), respectively . Totally, positive rates was 11%.
밀어(Rhinogobius brunneus)의 산란행동 및 초기생활사
문운기,나영언,안광국 한국육수학회 2005 생태와 환경 Vol.38 No.1
본 연구는 밀어 (Rhinogobius brunneus)의 산란행동 및 초기생활사를 밝히기 위하여 4가지 수온조건 하에서 실험을 수행되었다. 본 실험을 위해 밀어는 금강 지류중의 하나인 갑천에서 성숙된 수컷과 암컷을 채집하였으며, 일정기간의 순치과정을 거친 후에 실험을 실시하였다. 본 실험 결과에 따르면, 성숙된 성어들의 구애행동 및 산란행동의 유형은 다음과 같이 요약된다. 밀어의 성숙된 수컷은 산란에 앞서 세력권을 형성한 후 자신의 세력권 안에 산란을 위한 둥지를 형성한 후, 성숙된 암컷을 유인 하여 둥지에 하부에 타원형 난을 단층으로 붙이는 부착 난 종으로 파악되었다. 수정된 알들은 수컷에 의해 보호 받는 종(Parental care species)임 이 관찰되었으며, 수정난의 평균 장경은 1.5±0.1 ㎜,단경은 0.7±0.1 ㎜였다. 실험실내 4개의 수온처리군에 따르면, 고온처리군 보다는 저온 처리군에서 부화가지 더 긴 시간이 소요되었으며, 높은 수온일수록 부화까지 소요되는 시간이 단축되는 것으로 나타났다. 또한, 18℃의 낮은 실험수온에서 부화된 자어는 약 3.6 ㎜의 전장을, 25℃ 이상의 높은 실험수온에서 부화된 자어는 3.1~3.2 ㎜의 전장을 보였다. 또한, 처리군에서 수온이 높을수록 빠른 성장을 보여 부화까지 소요되는 시간이 단축되는 결과를 보였으나, 부화 시 자어의 크기는 높은 수온 상태에서 보다는 낮은 수온상태에 서 부화된 개체가 큰 것으로 나타났다. 본 밀어에 대한 발생생태학적 기초연구는 수질오염 및 서식지 파괴에 의해 어종의 다양성이 빠르게 감소하는 상황에서 어류 개체군 보호 및 생태계보호에 기초자료를 제공할 것으로 사료된다. The objective of the study was to elucidate early life history of Rhinogobius brunneus, based on laboratory observations and field works, along with its courtship and spawning behaviors. The male and female fish matured were sampled for the lab experiments from Gap- Stream, which is one of the tributaries of Geum-River, Korea. The spawning behaviors and courtship of matured adults had a typical pattern; matured males of Rhinogobius brunneus built up spawning nests and had their own territory before the spawning, and the behavior then triggered the egg spawning of female with active courtship. The females attached elliptic egg mass as a single layer, on the stones below the spawning nest. After the spawning, the egg mass was guarded by males only until the hatching. The size of egg fertilized, measured by microscope was about 1.5±0.1 ㎜ in length and 0.7±0.1 ㎜ in the egg' s width. According to the lab experiments conducted under four temperature regime, the lapsed time for the hatching was shortened as temperatures increased in the treatments. On the contrary to the hatching time, the average length of newly hatched larvae showed a reverse relation with water temperature. The larvae, considered a final stage of the early life history was about 3.6 ㎜ in total length for water temperature of 18℃, whereas they were between 3.1 ㎜ and 3.2 ㎜ for over 25℃. This indicates that higher water temperature may decrease the growth rate in the early life history. These results may provide valuable information for the population conservation and ecosystem protection under accelerated water pollution and habitat degradations in Korea.
Toxoplasma 감염 가토의 임파구 아세포화 반응에 대한 연구
오창진,이영하,나영언,신대환 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2
This study was performed to observe the differences of lymphocyte blastogienic responses in rabbits infected with Fukaya and Me49 strain of Toxoplasma gondii. Rabbits were subcutaneously inoculated with mouse brain emulsion containing 50 cysts of each strain of Toxoplasma gondii. A total of 9 male rabbits was used. From the 1st week after inoculation, blastogenic responses of peripheral blood lymphocytes treated with concanavalin A (Con. A, 10.0㎍/ml), lipopolysaccharide(LPS, 25.0㎍/ml) and Toxoplasma lysate(20.0㎍/ml) respectively were examined by [methyl-^3H]-thymidine incorporation assay at l week interval for 8 weeks. The results obtained in this study were as follows: 1. The blastogenic responses of the Con. A-treated and Toxoplasma lysate-treated lymphocytes from rabbits infected with Fukaya and Me49 strain of Toxoplasma gondii respectively were significantly declined as compared with those of the contol rabbits(p<0.05). No significantly differences were revealed in the blastogenic responses of the experimental group each other. 2. The blastogenic responses of the LPS-treated lymphocytes from rabbits infected with Fukaya and Me49 strain of Toxoplasma gondii respectively were not significantly declined as compared with those of the contol mice. No significantly differences were revealed in the blastogenic responses of the experimental group each other. The results suggested that there were no significantly differences in the blastogenic responses to Con. A, LPS and Toxoplasma lysate of each strain of Toxoplasma gondii infected rabbits. But the experimental group was a significant depression in blastogenic responses to Con. A and Toxoplasma lysate as compared with those of control group.