금강유역에서 채집된 Lymnaea pervia, Lymnaeidae (애기물달팽이)외 3종(種)에 대한 계통유연성

나영언 建陽大學校 1998 建陽論叢 Vol.- No.6

RAPD(Randomly Amplified Polymorphic DNA) method was used to find out the phylogenetic relationships and the genetic polymorphisms on 4 species, C. chinensis, H. cantori, P. acuta and L. pervia collected in Keum-River basins. It was awared that 4 random primers among the used 20 primers were available to characterize the genetic relationships of 4 species freshwater gastropods. The data obtained from the above experiments were analyzed by PAUP Genetic Similarity Index Program. According to these analysis, the genetic similarity distance index was ranged from 0.5 to 0.9; that is it was revealed that C. chinensis was closely related to P. acuta and H. cantori to L. pervia as being treated to the specific primer sequences. From these results, RAPD analysis method could be succesively applied to the classification and genetic analysis for the phylogenetic relationships of the freshwater gastropods.

세종⦁대전시 일부 지역의 담수패류 분포와 유미유충 감염실태 조사

나영언,이재형,최인욱,이용석,김현주,이영하 한국패류학회 2017 The Korean Journal of Malacology Vol.33 No.3

To investigate the fauna and larval infection status of freshwater snails living in water stream and rice paddy, we collected 925 freshwater snails at 7 regions in Sejong and Daejeon at 2016 and 2017. From the survey, a total of 925 freshwater snails were identified as 6 families, 9 genus and 9 species. The collected ten species of freshwater mollusks were identified as Lymnaea pervia, Radix (Lymnaea) auricularia, Physa acuta, Cipangopaludina chinensis, Gyraulus convexiusculus, Hippeutis cantori, Segmentina hemisphaerula, Semisulcospira libertina, and Parafoussarulus manchuricus. The most populated snails was L. pervia and the second one was P. acuta. The cercariae of Paragonimus, Metagonimus and Echinostoma species were isolated from Semisulcospira, Gyraulus, Hippeutus, Parafoussarulus, Segmentina, Lymnaea and Radix collected at 3 regions among 7 surveyed regions. Collectively, this study presented that various species of freshwater snails and cercaria-infected snails are distributed at water stream and rice paddy at Sejong and Daejeon, and further investigation needs to be carried out to identify the fauna of freshwater snails in detail.

또아리물달팽이과(Planorbidae)의 분포, 분류 및 흡충류 유미유충 감염 실태

김종환,정우석,조경화,나영언 忠南大學校 環境問題硏究所 1994 環境硏究 Vol.12 No.-

The distribution, taxonomy of Planorbidid sanils and its cercarial emerging status were performed in the rice peddy field, irrigation canal, pond and lakes around Korean main four rivers (Hangang, Kumgang, Yongsangang and Naktonggang). The collected specimens of snails were identified into three genera and three species as Hippeutis cantori, Gyraulus convexiusculus, and Segmentina hemisphaerula on the basis of their shell feature and sexual organs. H. cantori was distributed around four river basins and the most predominant species, on the other side of Yongsangang, no G. corvexusculus was found. S. hemispaerula was collected all over the four river basins except in the middle and lower stream of Yongsangang. Electrophoretic analysis of protein from foot muscle extract revealed no remarkable differences among three Planorbid species, however the phylogenetic relationships of H. cantori was a remote ancestor than that of the G. convexiusculus and S. hemisphaerula by Isozyme analysis. Natural emerging rates of cercaria from the sanils : H. cantori observed 0.75%, G. convexiusculus 1.27% and S. hemisphaerula 0.11%, respectively. Five species of cercariae were classified as Fibricola seoulensis cercaria, Cercaria prosthogonimus, Cercaria misa, Echinostoma macrochis cercaria and one unidentified cercaria from H. cantori, the cercaria of E. macrochis was also found both snail species ; G. convexiusculus and S. hemisphaerula. Some physicochemical factors including water temperature, pH, electric conductivity, BOD, Ca++, Mg++ and pH of bottom sediment soil were determined at the habitats of Planorbid snail, those factors have not produced any notable influences on the inhabit and distribution of Planorbid snails.

밀어(Rhinogobius brunneus)의 산란행동 및 초기생활사

문운기,나영언,안광국 한국육수학회 2005 생태와 환경 Vol.38 No.1

본 연구는 밀어 (Rhinogobius brunneus)의 산란행동 및 초기생활사를 밝히기 위하여 4가지 수온조건 하에서 실험을 수행되었다. 본 실험을 위해 밀어는 금강 지류중의 하나인 갑천에서 성숙된 수컷과 암컷을 채집하였으며, 일정기간의 순치과정을 거친 후에 실험을 실시하였다. 본 실험 결과에 따르면, 성숙된 성어들의 구애행동 및 산란행동의 유형은 다음과 같이 요약된다. 밀어의 성숙된 수컷은 산란에 앞서 세력권을 형성한 후 자신의 세력권 안에 산란을 위한 둥지를 형성한 후, 성숙된 암컷을 유인 하여 둥지에 하부에 타원형 난을 단층으로 붙이는 부착 난 종으로 파악되었다. 수정된 알들은 수컷에 의해 보호 받는 종(Parental care species)임 이 관찰되었으며, 수정난의 평균 장경은 1.5±0.1 ㎜,단경은 0.7±0.1 ㎜였다. 실험실내 4개의 수온처리군에 따르면, 고온처리군 보다는 저온 처리군에서 부화가지 더 긴 시간이 소요되었으며, 높은 수온일수록 부화까지 소요되는 시간이 단축되는 것으로 나타났다. 또한, 18℃의 낮은 실험수온에서 부화된 자어는 약 3.6 ㎜의 전장을, 25℃ 이상의 높은 실험수온에서 부화된 자어는 3.1~3.2 ㎜의 전장을 보였다. 또한, 처리군에서 수온이 높을수록 빠른 성장을 보여 부화까지 소요되는 시간이 단축되는 결과를 보였으나, 부화 시 자어의 크기는 높은 수온 상태에서 보다는 낮은 수온상태에 서 부화된 개체가 큰 것으로 나타났다. 본 밀어에 대한 발생생태학적 기초연구는 수질오염 및 서식지 파괴에 의해 어종의 다양성이 빠르게 감소하는 상황에서 어류 개체군 보호 및 생태계보호에 기초자료를 제공할 것으로 사료된다. The objective of the study was to elucidate early life history of Rhinogobius brunneus, based on laboratory observations and field works, along with its courtship and spawning behaviors. The male and female fish matured were sampled for the lab experiments from Gap- Stream, which is one of the tributaries of Geum-River, Korea. The spawning behaviors and courtship of matured adults had a typical pattern; matured males of Rhinogobius brunneus built up spawning nests and had their own territory before the spawning, and the behavior then triggered the egg spawning of female with active courtship. The females attached elliptic egg mass as a single layer, on the stones below the spawning nest. After the spawning, the egg mass was guarded by males only until the hatching. The size of egg fertilized, measured by microscope was about 1.5±0.1 ㎜ in length and 0.7±0.1 ㎜ in the egg' s width. According to the lab experiments conducted under four temperature regime, the lapsed time for the hatching was shortened as temperatures increased in the treatments. On the contrary to the hatching time, the average length of newly hatched larvae showed a reverse relation with water temperature. The larvae, considered a final stage of the early life history was about 3.6 ㎜ in total length for water temperature of 18℃, whereas they were between 3.1 ㎜ and 3.2 ㎜ for over 25℃. This indicates that higher water temperature may decrease the growth rate in the early life history. These results may provide valuable information for the population conservation and ecosystem protection under accelerated water pollution and habitat degradations in Korea.

얼룩동사리(Odontobutis interrupta)의 산란행동 및 난발생에 관한 연구

최신석,나영언 한국환경생물학회 2000 환경생물 : 환경생물학회지 Vol.18 No.3

얼룩동사리의 자연서식지 및 산란조건을 관찰한 바 수심은 0.3∼0.6m 범위, 유속은 0.1∼0.3m/sec, 난괴의 정착면적은 2.75∼19.56㎝였다. 난소의 성숙도는 체장 80㎜ 이상에서 점점 증가하며 100㎜ 정도 되어서는 최고의 성숙도를 보였다. 또한 산란행동은 인공수조에서 수컷이 산란장소를 마련한 후 강한 세력권을 형성하고, 암컷을 유인한 후 방란 촉진 구애행동을 보였다. 그리고 난 후 배를 거꾸로 뒤집은 자세로 방란 방정을 마친 후 암컷을 쫓아냈으며 가슴지느러미를 이용하여 수류를 일으킴으로써 알에 신선한 물을 공급하였다. 난발생과정은 수정 후 1시간 17분에 배반이 형성되고 이후 약 36분의 간격으로 난할이 규칙적으로 일어났으며 7시간 12분에 포배기, 11시간 11분에 낭배기가 시작, 32시간 48분에 난황전을 형성하였다. 수정 후 33시간 45분에 배체가 형성되었고, 47시간 27분에 안포가 형성되며 체절은 30∼31개가 되었다. 65시간 15분에 심장의 원기가 형성되고 박동수는 44∼48time/min이 되었다. 수정 후 138시간 40분에 가슴지느러미가 형성되었고, 170시간 45분에 부레의 형성과 자어의 몸통하단에 흑색소포가 관찰되었다. 수정후 248시간 05분에 난각을 뚫고 부화하였으며 이때 자어의 전장은 5.8±0.2㎜, 무게는 3.0±0.5㎎이었다. 몸음 투명하며 흑색소포가 산재하여 있었고 난황이 남아 있었다. Early life history and spawning for Odontobutis interrupta were observed in the laboratory during May-August 1999 with a condition of natural habitats in the field. Optimal water temperature for spawning was between 17.5 and 22.0℃ and appropriate water depth and current velocity in the natural habitat ranged 0.3∼0.6m and 0.1∼0.3m/sec, respectively. Ovary maturation index peaked at about 100㎜ in the total length and their values gradually decreased after the size. Male fishes showed a territory and courtship behavior to adult females and the males frequently pushed upper-ventral part of females for egg releases. After females spawned, the males guarded the egg masses and supplied dissolved oxygen using pectoral fins. According to observation of egg development in the laboratory, blastodisc formed in 1hr 17 min after the fertilization, cleavaging at 36-minute interval regularly. Blastulation occurred in 7hr 12min after the fertilization, with gastrulation after 11hr 11mins and formation of york plug after 32hr 48min. Embryo was formed in 33hr 45min after fertilization and optic vesicles appeared in 47hr 27mins when 30∼31 somites were formed. Cardiac primordium was formed in 65 hr 15mins and its beat averaged 44∼48time/min. Pectoral fins were formed in 138hr 40min, air-bladder and black vesicles were observed in lower portion of young fish. Embryo hatched from she-ll membrane after about 10 days and juvenile was 5.8±0.2㎜ in total length, 3.0±0.5㎎ in weight.

外來患者 및 돼지에서의 間接Latex凝集反應法에 依한 Toxoplasma 抗體價 調査硏究

심희숙,나영언,이영하,신대환 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

This study was undertaken to konw the sero-epidemiologic meanings of toxoplasmosis by Indirect Latex Agglutination test of 514 general outpatients and 100 pigs. The results were obtained as follows: 1. Toxoplasma antibody titers were showed as 418 negative cases(81.3%), 63 cases(12.2%) in a titer of 1:16, 20 cases(3.9%) in a titer of 1:32, 8 cases(1.6%) in a titer of 1:64, 3 cases(0.6% ) in a titer of 1:128 and 2 cases(0.4%) in a titer of 1:256, respectively. 2. Antibody titer was somewhat declined to the nineteen ages (from 6.1% to 3,0%) but was increased from twenty to fifty-nine ages(from 9.1% to 24.2%). The positive percentage of Toxoplasma antibody titers were the highest as 24.2% in the fifties group. Totally, 33 cases(6.5%) among 514 patients was showed as positive reaction. 3. According to the sex group, 16 cases of the male and 17 cases of the female were showed as positive reaction 5.6%, 7.5%, respectively. Positive rates of the female was somewhat higher than that of the male. 4. Antibody titers of sera of the pig were shown as 65 negative cases(65%), 24 cases in a titer of 1:16(24%), 6 casesin 1:32(6%), 3 cases in 1:64(3%) and 2 cases in 1:1.28(2%), respectively . Totally, positive rates was 11%.

錦江流域 淡水魚의 Sparganum에 關한 調査硏究

辛大煥,羅榮彦,權斗星 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

Prevalence of the plerocercoid larva in the fresh-water fishes in Keum-river basin was surveyed from April to September, 1986. To observe whether this plerocercoid larva is really sparganum or not, and can be transfered to the paratenic host or not, a study was performed with the adequate experimental animal (domestic fowl and cat). It was collected the 1096 fresh-water fishes that was ranged 8.0^-16.0cm in length. The results obtained were as follows; 1. A number of ova of Diphyllobothrium spp, was confirmed under the microscopic study, 5 days after experimental infection through the mouth, and them sacrificed to the experimental animal (final host, cat). The adult worm of Diphylobothrium spp., 9.5cm in length, was obtained and that worm was located in the upper position of rectum, Therefore, this plerocercoid larva was confirmed as Sparganum. 2. Transference to the paratenic host (dowestic fowl) was not resulted. 3. Among the total number of 1096 fresh-water fishes, only the Z. platypus was infected with 107 worms corresonding to 9.8%. 4. Infection rate according to the length of Z. platypus was 66. 4% at 12-13. 9cm in length, and this percentage was the highest prevalent rate.

Cortisone acetate 투여한 마우스의 Pneumocystis carinii 감염양상 및 세포매개성 면역반응에 관한 연구

김충현,이영하,나영언,신대환 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

This study was attempted to find the cell-mediated immune responses and infection aspects of Pneumocystis carinii in immunosuppressive mice. The experimental groups were administered the suppressive regimen consisting of cortisone acetate 2.5mg injected subcutaneously twice a week, a low(8%) protein diet, and tetracycline in the drinking water(1mg/ml) for 10 weeks. From the 1st week after cortisone acetate injection, the number of P. carinii cysts in the lung specimens were examined by Gomori's methenamine silver stain at 1 week interval for 10 weeks. And the blastogenic response of spleen cells treated with Con. A (5.0㎍/ml) was examined by [^3H]-thymidine incorporation assay at the same time. The results obtained in this study were as follows: 1. The number of P. carinii cysts were remarkably increased in the lungs from 2 weeks to 4 weeks, and thereafter was slowly increased throughout the experiment. 2. The blastogenic response of Con. A treated-splenocytes from mice injected with cortisone acetate was significantly declined as compared with those of the control mice(p<0.05). 3. There were not correlation between the number of P carinii cysts in the lung specimens and the blastogenic responses of splenocytes to Con. A(correlation coefficient, r=0.38285, p<0.2749).

Cortisone acetate 投與에 依한 Pneumocystis carinii 肺炎의 發症 課程

閔庚璇,李英河,羅榮彦,辛大煥 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

This study was undertaken to determine the provocative characteristics of experimental Pneumocystis carinii pneumonia in cortisone acetate treated rats. A Total of 55 male Sprague-Dawley rats, body weights 166-224g, were used. Five out of them were used as control group and remaining 50 were as experimental groups. In experimental groups, the rats were injected with 25mg of cortisone acetate twice a week for 10 weeks subcutaneously and drinking water contained 1000mg tetracycline hydrochloride per liter. Control group was not administered any drug. The 5 rats were sacrified weekly at each week in experimental group. but control group was sacrified at 10 weeks. For arterial blood gas analysis, blood was collected from carotid artery of rats using catheter. The rat's lung were removed after killed. The stamp smeared specimens of lungs were prepared and stained toluldin blue 0 for the quantification of P.carinii cysts. For the histopathologic observations, lungs were fixed in 10% buffered neutral formalin, cut into sections and stained with hematoxylin-eosin stain. For the histologic score of the intensity of P.carinii pneumonia, the sections were stained with Gomori's methenamine silver stain. The results obtained were as follows : 1. In the arterial blood gas analysis of cortisone acetate treated rats, blood pH and PaCO_2 was showed normal range. But PaO_2 was slightly decreased in experimental period. 2. Histopathologic finding of H-E staining sections were found in order of infiltration of lymphocyte and alveolar macrophage, eosinophilic foamy exudate, thickening of alveolar septum, desquamation of pneumocyte and fibrinoid degeneration and so on, but plasma cell was not found. 3. The histologic score of the intensity of P.carinii pneumonia was increased in proportion to the cortisonized periods of rats. 4. The number of cysts in the stamp smeared specimens of lungs were increased in proportion to the cortisonized periods of rats. And highly significant correlation was found between the histologic score of the intensity of P.carinii pneumonia and the number of cysts countered in the stamp smeared specimens of lungs(r=0.8620, ANOVA test p<0.0001). The present study revealed that provocative characteristics of experimental P. carinii pneumonia was highly significant relationship with the histologic score of P. carinii pneumonia and the number of cysts in the stamp smeared specimens of lung.

Toxoplasma 감염 가토의 임파구 아세포화 반응에 대한 연구

오창진,이영하,나영언,신대환 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

This study was performed to observe the differences of lymphocyte blastogienic responses in rabbits infected with Fukaya and Me49 strain of Toxoplasma gondii. Rabbits were subcutaneously inoculated with mouse brain emulsion containing 50 cysts of each strain of Toxoplasma gondii. A total of 9 male rabbits was used. From the 1st week after inoculation, blastogenic responses of peripheral blood lymphocytes treated with concanavalin A (Con. A, 10.0㎍/ml), lipopolysaccharide(LPS, 25.0㎍/ml) and Toxoplasma lysate(20.0㎍/ml) respectively were examined by [methyl-^3H]-thymidine incorporation assay at l week interval for 8 weeks. The results obtained in this study were as follows: 1. The blastogenic responses of the Con. A-treated and Toxoplasma lysate-treated lymphocytes from rabbits infected with Fukaya and Me49 strain of Toxoplasma gondii respectively were significantly declined as compared with those of the contol rabbits(p<0.05). No significantly differences were revealed in the blastogenic responses of the experimental group each other. 2. The blastogenic responses of the LPS-treated lymphocytes from rabbits infected with Fukaya and Me49 strain of Toxoplasma gondii respectively were not significantly declined as compared with those of the contol mice. No significantly differences were revealed in the blastogenic responses of the experimental group each other. The results suggested that there were no significantly differences in the blastogenic responses to Con. A, LPS and Toxoplasma lysate of each strain of Toxoplasma gondii infected rabbits. But the experimental group was a significant depression in blastogenic responses to Con. A and Toxoplasma lysate as compared with those of control group.