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섬유질계 동시당화발효를 위한 내열성 융합 효모, Kluyveromyces marxianus CHY1612의 개발
강현우(Hyun-Woo Kang),김율(Yule Kim),박주용(Ju-Yong Park),민지호(Jiho Min),최기욱(Gi-Wook Choi) 한국생물공학회 2010 KSBB Journal Vol.25 No.6
To develop thermostable ethanol fermentative yeast strain for lignocellulosic simultaneous saccharification and fermentation, high ethanol producing yeast, Saccharomyces cerevisiae CHY1012 and thermostable yeast, Kluyveromyces marxianus CHY1703 were fused by protoplast fusion. The thermostable fusant, CHY1612 was identified as a Kluyveromyces marxianus by phenotypic and physiological characteristics, as well as molecular analysis based on the D1/D2 domains of the large subunit (26S) rDNA gene and the internally transcribed spacer (ITS) 1 + 2 regions. For lignocellulosic ethanol production, AFEX pretreated barley straw at 150℃ for 90 min was used in a simultaneous saccharification and fermentation (SSF) process using thermotolerant CHY1612. The SSF from 16% pretreated barley straw at 43℃ gave a saccharification ratio of 90.5%, a final ethanol concentration of 38.5 g/L, and a theoretical yield of 91.2%. These results show that K. marxianus CHY1612 has potential for lignocellulosic ethanol production through simultaneous saccharification and fermentation with further development of process.
객체지향형 실시간 EMS 데이터베이스 구축을 위한 개념디자인
최상열,김종형,신명철,김응모,김학만 성균관대학교 1997 학술회의지원논문목록집 Vol.1997 No.-
Currently, EMS(Energy Mangement System) database is implemented by relational concept but, It is hard to describe the characteristic of power system data which require real time management and composite type. This paper present the way how to design conceptual schema for EMS database using ovject-oriented concept which is free to decribe composite data type and support inheritance concept.