감맥대조탕(甘麥大棗湯)이 적출 흰쥐 심장의 혈역학적(血力學的) 기능(機能)에 미치는 영향(影響)

김덕곤,박성남,Kim, Deog-Gon,Park, Sung-Nam 대한한방소아과학회 2007 대한한방소아과학회지 Vol.21 No.1

Objectives : In order to verify the cardiovascular hemodynamic function of Kammaegdaejo-tang, the experiment was performed in the rats. Methods : Twelve hearts removed from male Sparague-Dawley rats weighing between 250g and 300g were perfused by the Langendorff technique with modified 37 Krebs-Henseleit's buffer solution at a constant perfusion pressure. They were randomly assigned to one of two groups, supplied with either normal saline or Kammaegdaejotang administration. Heart rate, left ventricular pressure, +dp/dt maximum, -dp/dt maximum, and -dp/dt/ +dp/dt ratio were evaluated at baseline after the administration of either normal saline or Kammaegdaejotang. Results : Kammaegdaejotang made the heart rate increasing significantly (p<0.05). Kammaegdaejotang did not effectively work on left ventricular pressure of the isolated heart(p=0.11, no significance). The significant effects of Kammaegdaejotang were observed on +dp/dt max and -dp/dt max(p<0.05). Kammaegdaejotang did not effectively work on -dp/dt/ +dp/dt ratio(p=0.24, no significance).

우방자(牛蒡子)가 항알러지 염증반응에 미치는 영향

김덕곤,이진용,남지영,Kim, Deog-Gon,Lee, Jin-Young,Nam, Ji-Young 대한한방소아과학회 2006 대한한방소아과학회지 Vol.20 No.1

Objective : Allergic Inflammation is related with secretion of Cytokine. This study was performed to examine the effects of Woobangja on anti-allergic inflammation. Method : While macrophage 264.7cells was chosen as a normal group a control group was classified into three groups. One was stimulated with LPS. and another was pretreated with Woobangja for 1 hour. The third was pretreated with gydrocortisone for 1 hour. After the pretreatment, macrophage were incubated with lipopolysaccharide(LPS) 100 ng/ml for 12h and media collected and $TNF-{\alpha}$, IL-6, $IL-1{\beta}$, IL-10 concentration in supernatants were measured each by Enzyme linked immuno-sorbent assay. Woobangja were used $50\;{\mu}g/ml$, $100\;{\mu}g/ml$, $250\;{\mu}g/ml$, $500\;{\mu}g/ml$, 1 mg/ml. Hydrocortisones were used respectively $10^{-8}\;M$,$10^{-7}\;M$,$10^{-6}\;M$,$10^{-5}\;M$,$10^{-4}\;M$. Results : Woobangja showed inhibitory effect on $TNF-{\alpha}$ by LPS-stimulated macrophage 264.7. The inhibitory effect was most significant in 1mg/ml(p<0.01), and has increased according to the number of doses. Woobangja also showed inhibitory effect on IL-10 by LPS-stimulated macrophasg 264.7. The inhibitory effect was most significant in $100\;{\mu}g/ml$, and was not in a dose-dependent manner as Hydrocortisone group. Woobangja and Hydrocortison showed contrary effect on $IL-1{\beta}$ in al five concentration(p<0.01), and at the lowest concentration ($50\;{\mu}g/ml$) the level of $IL-1{\beta}$ was the lowest. On the other hand hydrocortison was observed to have inhibitory effect on $IL-1{\beta}$ in all five concentration(p<0.01). IL-6 was inhibited by hydrocortison in a roughly dose-dependent manner, but was not inhibited by Woobangja. On the contrary Woobangja obviously increased the expression of $IL-1{\beta}$ in all five concentration(p<0.01), but it was not related with concentrations. Conclusion : 1. Woobangja does significantly inhibit the expression of $TNF-{\alpha}$ by LPS-stimulated macrophage 264.7. 2. Woobangja does significantly increse the expression of IL-6 by LPS-stimulated macrophage 264.7. 3. Woobangja does significantly increse the expression of $IL-1{\beta}$ by LPS-stimulated macrophage 264.7. 4. Woobangja does significantly inhibit the expression of IL-10 by LPS-stimulated macrophage 264.7. 5. Woobangja is observer to have anti-allergic inflammatory effect through inhibiting inflammatory cytokine.

Methotraxate로 유발된 생쥐의 면역반응(免疫反應)에 대한 당귀육황탕(當歸六黃場)과 옥병풍산가미방(玉屛風散加味方)의 효과(效果)

김덕곤,김경선,Kim, Deog-Gon,Kim, Gyoung-Sun 대한한방소아과학회 2007 대한한방소아과학회지 Vol.21 No.1

Objectives : In order to study the effect of Dangkwiyughwangtang and Okbyoungpoongsangamibang on the immune response induced by methotrexate in mice. Method : Delayed type of hypersensitivity, hemagglutinin titer, hemolysin titer, rosette forming cells, phagocytic activity for immune response, lymphocyte transformation, and productivity of Interleukin-2 were measured. Results : Body weight decreasing was significantly inhibited as compared with control group in both the Dangkwiyughwangtang and Okbyoungpoongsangamibang groups. Delayed type of hypersensitivity was significantly increased as compared with control group in both groups Hemagglutinin titer was significantly increased as compared with control group in both groups. Hemolysin titer was significantly increased as compared with control group in the Okbyoungpoongsangamibang group. Rosette forming cells were significantly increased as compared with control group in both groups. Phagocytic activity for immune response was slightly decreased in the Dangkwiyughwangtang group and slightly increased in the Okbyoungpoongsangamibang group insignificantly as compared with the control group. Lymphocyte transformation was significantly increased as compared with the control group in both the Dangkwiyughwangtang and Okbyoungpoongsangamibang groups. Productivity of Interleukin-2 was significantly increased as compared with the control group in both the Dangkwiyughwangtang and Okbyoungpoongsangamibang groups. Conclusion : Both the Dangkwiyughwangtang and Okbyoungpoongsangamibang groups enhance immunity in mice.

방풍통성산가미방(防風通聖散加味方)이 비만유도(肥滿誘導) 백서(白鼠)에 미치는 영향(影響)

김덕곤,김병우,Kim, Deog-Gon,Kim, Byung-Woo 대한한방소아과학회 2007 대한한방소아과학회지 Vol.21 No.1

Objectives : These experimental studies were designed to investigate the effects of Bangpoongtongsungsankamibang on the weight and the lipid metabolism of the obese rats induced by 2% cholesterol diet. They were designed to change values in the serum and leptin levels in the rats. Materials and Methods : The serum and leptin levels were measured for a period of 8 weeks for obese SD rats induced by 2% cholesterol diet. Results : The body weight showed a tendency to decreased significantly in sample (p<0.05). The Total cholesterol and Triglyceride level significantly decreased in sample (p<0.05). The HDL Cholesterol levels significantly increased in sample (p<0.05). The LDH levels did not show a significant change in sample. The ALP levels significantly decreased in sample (p<0.05). The SGOT and SGPT level did not show a significant change in sample. The Leptin levels significantly decreased in sample (p<0.05). The average size of the epididymal fat cell significantly decreased in sample (p<0.05).

중학생 비만아들의 생활습관과 정신상태의 상관관계

김덕곤,Kim, Deog-Gon 대한한방소아과학회 2010 대한한방소아과학회지 Vol.24 No.1

Objectives The relationship between depression, obesity, life style of adolescents has been reported, but the results were not consistent. Thus this study was designed to find out the relationship. Methods The participants were the first and second grade middle school students. The participants were asked to measure their height and weight etc, and were asked to survey questionnaires. To diagnose the students' degree of depression, Children's Depression Inventory(CDI) by Kovas and Beck. Results The average depression score in the obese group was significantly higher than that of the normal body weight group. In addition, male obese group scored higher on CDI than the female obese group, and the obese group of participants who don't exercise, but like to play computer games and watch TV scored higher on CDI than the group who exercise and don't play computer games. Conclusions This study proved that depression in adolescent was closely related with obesity and obesity-related lifestyles. "The research was supported by the Kyung Hee University Research Fund in 2007"(KHU-20071638).

한국한의학과 인도전통의학의 비교연구

김덕곤(Deog-Gon Kim) 대한한의학회 2005 대한한의학회지 Vol.26 No.2

Sensitiv Imago^TM 측정에 의한 틱 장애 환아의 검사 결과 보고

김민주,김덕곤,이진용,Kim, Min Joo,Kim, Deog Gon,Lee, Jin Yong 대한한방소아과학회 2014 대한한방소아과학회지 Vol.28 No.3

Objectives The purpose of this study is to report test results in eight tic disorder children using Sensitiv $Imago^{TM}$. Methods Eight tic disorder children were tested using Sensitiv $Imago^{TM}$. Also, they were diagnosed with tic disorders by DSM-IV (Diagnostic and Statistical Manual of Mental Disorders, $4^{th}$ edition) and we evaluated tic disorder cases by Yale Global Tic Severity Scale (YGTSS). Results In 8 cases, 1 case was transient tic disorder, 3 cases were Tourette's disorder and 4 cases were unspecified tic disorder. In the result of Sensitiv $Imago^{TM}$, 4 cases showed the lowest score at filter ${\sharp}5^*$ and 4 cases showed the lowest score at filter ${\sharp}6^*$ in [Express Monitoring] of [Review of System Disorders of Homeostasis]. Filter #5 includes urogenital organs, liver, gallbladder, kidneys, urinary bladder and ureter and Filter #6 stands for organs of immune and respiratory systems. Conclusion We report test results in eight tic disorder children using Sensitiv $Imago^{TM}$. Further studies about the principle, repeatability, reproducibility of Sensitiv $Imago^{TM}$ are needed.

소아기관지(小兒氣管支) 천식(喘息)의 임상적(臨床的) 고찰(考察 ) (제(第)II보(報))

정규만,김덕곤,이동현,Jeong Gyu-Mann,Kim Deog-Gon,Lee Dong-Hyun 대한한방소아과학회 1986 대한한방소아과학회지 Vol.1 No.1

During 34 months from October 1982 to July 1985, a clinical study was made on 217 cases of out-patients with pediatric bronchoarthma at Kyung-Hee University Oriental Hospital. ?The observed results were as follows; ?1. In the age of the patients ranged from six months to twelve years; they were almost under six years (73.1 %) The ratio of male to female was 3: 1. ?2. In case of the age to be attacked with bronchoarthma, the age 3 covered 43.9% (95 cases), and the ages of less than 6 years 86.3% (187 cases); and, especially, the age 2 - 3 covered the highest rate (18.4%; 40 cases) ?3. The contraction period of bronchoarthma was almost less than one year (70.1%; 152 cases); especially, less than one week covered the highest rate (22.1 %; 48 cases) ?4. In case of the contraction season; winter (December to February) had the highest rate (33.2%; 72 cases), and fall, spring and summer came after it. And 13 cases (6%) were attacked in all seasons. ?5. Of a day, the severe symptom was shown most frequently from 6 pm to midnight (31.3%; 68 cases), and next from midnight to 6 am (29.1%; 62 cases) ?6. The common symptoms of out-patients were productive cough (24.0%; 147 cases), easily-catch-cold (22.5%; 138 cases), dry cough (11.4%; 70 cases), and so on. ?7. In case of the history of the patients, upper respiratory infections covered 52.1 % (113 cases) bronchitis(29.0%; 63 cases), pneumonia(17.1%; 37 cases), and tonsilitis& pharyngitis(6.0%; ?13 cases): and allergic symptoms covered (42 cases: 19.3%) fetal fever & eczema (11.5%: 25 cases), and allergie Rhinitis (7.8%: 17 cases) ?8. The family of the patients were shown to have the history of bronchitis (21.6%; 47 cases), bronchoarthma (21.2%; 46 cases), and tuberculosis (16.6%; 36 cases) ?9. The factors of bronchoarthma were shown as upper respiratory infections (38.3%; 38 cases), cold weather (18.9%; 41 cases), and exercise (175%; 38 cases) ?10. Of the treatment periods of the patients, less than one month (69.2%; 150 cases) took the highest rate, in which less than a week was 33.2% (72 cases) and one or two weeks 15.2% (33 cases) ?11. The main prescriptions were kunpyunetang (79 cases; 17.0%), Agoayangyuegunpyuetang (73 cases; 15.7%) and Haepyoyangjintang (72 cases; 15.6%) ?12. In the results of treatment, 132 cases (60.9%) was improved; especially, subjective signs of 33 cases (15.2%) of them, was almost removed.

신생아 B형 간염 예방 접종 후 하지 부종이 발생한 환아 치험 1례

한주희,김덕곤,이진용,Han, Ju Hui,Kim, Deog Gon,Lee, Jin Yong 대한한방소아과학회 2014 대한한방소아과학회지 Vol.28 No.1

Objectives The purpose of this study is to report the clinical effects of oriental medical treatment on Extensive limb swelling which is rare adverse reaction of vaccination. Methods A 11 months old infant who appealed unilateral thigh swelling after immunization and signs of local inflammation was administered a complex herbal medicine consisting of five decoctions; Gwakhyangjunggisan, Binsosan, Danggwisusan, Samchulgunbitang and Gwibitang. The length of the constant regions of the thigh circumference was measured every visit. Each process was recorded by photograph. After one year from the first visit, phone call was conducted to follow-up. Results The length of the thigh after administration of herbal medicine did not change much, but the part where swelled hard became little bit softer. A year later, patient's growth and walking was normal but the swollen part not changed. Since then the similar adverse effects did not recur on booster doses vaccines. Conclusions The adverse reactions of vaccination are rarely reported and there is no specific treatment for swelling from Hepatitis B vaccine. It will be more helpful to study if more cases reported about any adverse drug reactions or any side effects from the immunization.

우방자(牛蒡子)가 아토피 피부염에 미치는 영향

이진용,김덕곤,한규철,Lee, Jin-Yong,Kim, Deog-Gon,Han, Kyu-Chul 대한한방소아과학회 2004 대한한방소아과학회지 Vol.18 No.2

Objective : Atopic dermatitis has a close relationship with degranulation of mast cell and separation of Histamine. As there's no experiment with herb, using Arctii Fructus, we investigated experimental influence of Arctii Fructus on degranulation of mast cell and separation of histamine in SD rat. Methods : The SD rats are classified into three groups. One group is normal one treated by normal saline before medical treatment. The other is control group prescribed to Compound 48/80 before normal saline treatment. And the third is experimental group prescribed to compound 48/80 after medical treatment of Arctii Fructus. Then, I investigated the experimental results by measuring the degree of degranulation and separation of histamine. The results of investigation on SD rat group showing the degree of inhibitory effect of degranulation of a mast cell are as follow, the normal group treated by normal saline reflecting the degree of degranulation is $6.10{\pm}0.20\;%$, the control group treated by only compound 48/80 is $87.56{\pm}11.00\;%$, the experimental group which treated by compound 48/80 and Arctii Fructus's medical treatment is $16.26{\pm}4.67\;%$. Results : The normal group treated by only normal saline reflecting the degree of degranulation is $6.10{\pm}0.20\;%$, the control group treated by only compound 48/80 is 87.56=11.00 %, the experimental group treated by compound 48/80 and Arctii Fructus's medical treatment is $16.26{\pm}4.67\;%$. This result indicates that the degree of degranulation of mast cell is obviously inhibited (p<0.0l) in the experimental group in comparison with control one. The analysis of data obtained from plasma, which collected from the experimented SDrats' hearts before their death, and the measurement of quantity of histamine secretion show the following results. The quantity of normal group and control one is $25.34{\pm}4.58$ nM, $348.59{\pm}30.77$ nM respectively, and experimental one prescribed to compound 48/80 after medical treatment of Arctii Fructus is $263.56{\pm}21.34$ nM. This result indicates that separation of histamine isobviously inhibited in the experimental group in comparison with control one (p<0.05). Conclusions : Arctii Fructus does obviously inhibit the degree of degranulation of mast cell (p<0.0l) and separation of histamine in the plasma (p<0.05).