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- 저자 : 권택부 ( Taek Boo Kwon ) (검색결과 2 건)
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한의학(韓醫學)에서 분류하는 사상체질(四象體質)에 따른 이식항원(移植抗原) HLA-DR 유전자(遺傳子) polymorphism에 관한 연구
권택부(Taek Boo Kwon),황치원(Chee Won Whang),설인찬(In Chan Sul),이경옥(Kyung Ok Lee),황규원(Kyu Yoon Hwang),양동호(Dong Ho Yang),홍세용(Sae Yong Hong) 대한내과학회 2000 대한내과학회지 Vol.58 No.1
N/A Background : In Korean traditional medicine, human beings are classified into 4 groups accordance with the characteristics of physical constitution, named ; Taeum, Taeyang, Soum, and Soyang. Most of Korean traditional doctors believe that the back ground of pathophysiology is differ among each group in many diseases. HLA is the most polymorphic gene and related with human immune response. It has been used for determination of individual identification, genetic diversity and a study of disease association in different ethnic group. It has well known that the most human inflammatory disease which thought to have some autoimmune basis are in some way promoted by genes of the HLA region. The purpose of this study is to observe the distribution of HLA-DR genotypes in 4 types of physical constitution in Korean traditional medicine. Methods : Three hundred and ten Korean subjects(Soyang : 58cases, Soum :142cases, Taeum : 92cases, Taeyang : 18cases) were enrolled for this study. Genomic DNAs extracted by phenol/chroloform were amplified with allele specific primers and determined by 2% agarose gel electrophoresis with ethidium bromide staining for analysis of HLA-DR polymorphism. Results : The genotype frequency of HLA-DR in a total or 310 Koreans was DR1 ; 7.2%, DR 2; 10.5%, DR 3;1.7%, DR 4; 19.9%, DR 5; 11.9%, DR 6;19.9%, DR 7; 7.0%, DR 8; 10.6%, DR 9; 8.7%, DR10;1.9%. There was no significant diffrence of HLA-DR genotype distribution among four physical constitution groups classified by Korean traditional medicine. Conclusion : Our results suggest that the classification of physical constitution in Korean traditional medicine is not related to HLA-DR genotypes.(Korean J Med 58:91-101, 2000)
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HPLC-MSD를 이용한 식육 중 합성항균제의 동시분석
홍인석 ( In Suk Hong ),최윤화 ( Yoon Hwa Choi ),권택부 ( Taek Boo Kwon ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.3
This study was conducted to develop the analytical method about simultaneous determination for synthetic antimicrobials in muscle by high performance liquid chroma-tography-mass selective detector(HPLC-MSD). Solid phase extraction(SPE), matrix solid phase dispersion(MSPD) and liquid-liquid extraction(LLE) have been adapted as pretreatment procedures for HPLC-MSD. Among various solvent tested, methanol was chosen for extraction of synthetic antimicrobials in muscles. For the optimized response, the values of various MS parameters including fragment voltage, drying gas flow, nebulizer pressure, drying gas temperature were verified. The average recovery rates using MSPD and SPE for muscles of bovine and pork were 78.9-127.1% and 78.3-121.7%, respectively. This method was verified the satisfactory performance for fourteen synthetic antimicrobials excepting carbadox in muscle of pork as detection limit of 0.05 ㎍/g on API/ES SIM mode.
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