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Hur, Sung-Pyo,Lim, Bong-Soo,Hwang, In-Joon,Kim, Se-Jae,Ryu, Yong-Woon,Hur, Sang-Woo,Song, Young-Bo,Jeong, Hyung-Bok,Baek, Hae-Ja,Takemura, Akihiro,Lee, Young-Don The Korean Society for Integrative Biology 2012 Animal cells and systems Vol.16 No.2
We investigated the effects of fadrozol, an aromatase inhibitor (AI), and $17{\alpha}$-methyltestosterone (MT) on the induction of sex change in juvenile longtooth grouper $Epinephelus$ $bruneus$, via histological observation of gonads. Changes in the mRNA expression of GtH subunits (FSH-${\beta}$ and LH-${\beta}$) in the pituitary, and estradiol-$17{\beta}$ (E2) and 11-ketotestosterone (11-KT) levels in the blood were also surveyed after AI and MT treatment. Juvenile longtooth groupers ($113{\pm}17g\;body\;weight$; $16.2{\pm}1.2cm\;body\;length$) received intramuscular injections of AI at 3 (3-AI) and 5 (5-AI) mg/kg BWdoses and MT at a 5 mg/kg BW (5-MT) dose. At week 7 post-injection, 3-AI and 5-MT oocytes were degenerated, and gonads of the 5-AI group initiated spermatogenesis. At week 21 post-injection, 3-AI- and 5-MT-treated gonads contained spermatogonia and spermatocytes, while 5-AI treatment induced advanced stages of spermatogenesis. The serum E2 level showed no significant differences throughout the experimental period, whereas that of 11-KT was significantly elevated in the 5-AI group at weeks 7 and 21 post-injection. A significant increase in the expression of FSH-${\beta}$ mRNA was evident in the 5-AI group at week 21 post-injection. In contrast, LH-${\beta}$ mRNA expression did not significantly differ among groups during the experimental period. These results imply that sex change has two stages in the longtooth grouper. In the first stage, oocytes are degenerated by the stimulation by 11-KT, and in the second stage spermatogenesis occurs, owing to the co-effects of 11-KT and FSH-${\beta}$.
The Dairy Calves Mortality : the Causes of Death during Ten Yearsin a Large Dairy Farm
Tai Young Hur,Young–,Hun Jung,Seog–,Jin Kang,Chang yong Choe,Ui Hyung Kim,Il Sun Ryu,Dong Soo Son,Kwang Soo Baek,Kwang Seok Ki,Hyun June Lee,Eung Gi Kwon,Guk Hyun Suh,Young Il Cho 대한수의학회 2012 대한수의학회 학술대회발표집 Vol.2012 No.-
난질과 유생발생을 이용한 4개 멍게(Halocynthia roretzi) 어미계군 특성 비교
허영백 ( Young Baek Hur ),김은경 ( Eun Kyung Kim ),임영섭 ( Young Seob Lim ),전창영 ( Chang Young Jeon ),조기채 ( Kee Chae Cho ),명정인 ( Jeong In Myeng ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.5
To compare four populations of sea squirt Halocynthia roretzi adults, their egg quality and larval development were investigated in the hatchery. The populations were: south sea wild (SSW), south sea cultured (SSC), east sea wild (ESW) and Iwate Japan cultured (IJC). Egg quality and larval development were compared using 13 factors (fertilization rate and diameter, proximate composition, amino acid and fatty acid contents of eggs, hatching rate and various sizes of tadpole larvae) which were obtained from each population. Fertilized egg diameter, hatching rate and size of tadpole larvae were significantly different among the four populations (P<0.05). Eggs were produced with higher crude protein (5.20±0.00% SSW, 4.71±0.01% ESW, 4.66±0.01% SSC and 3.96±0.01% IJC) and lipid (1.22±0.01% ESW, 1.01±0.00% SSW, 0.77±0.01% SSC and 0.69±0.00% IJC,) contents from domestic wild populations than from Japanese or cultured populations. Also amino acid and fatty acid contents were different. The extent of similarity between domestic and Japanese populations (30.5% IJC:SSW, 34.3% IJC:SSC and 40.7% IJC:ESW) was relatively low but was very high between SSW and SSC (73.9%). These results may have been due to differences in the abundance of food types and environmental conditions in the four localities and consequent differences in the diets of the sea squirts.
2010년 국내 남해 해역에 출현한 Pseudo-nitzschia cf. brasiliana의 형태 특성
허영백 ( Young Baek Hur ),박종규 ( Jong-gyu Park ) 한국환경생물학회 2014 환경생물 : 환경생물학회지 Vol.32 No.4
규조류 Pseudo-nitzschia는 아미노산의 일종 신경독인기억상실성 패독 domoic aicd (DA)를 생산하여 주목받고있는 속이다. 최근 2010년 여름철에 남해 EEZ 골재채취단지와 거문도에서 Pseudo-nitzschia cf. brasiliana가 최고우점종으로 출현하여 그 형태 특성을 관찰하였다. P. cf.brasiliana는 개각(valve)의 모양이 선형이었고, 길이와 폭은 각각 51~76 μm, 1.9~2.6 μm이었으며 중간 공간(centralinterspace)은 없었다. 10 μm 안의 등줄안다리(fibulae)수와 점무늬열(striae) 수는 각각 30~37, 31~37이었다.미소공(poroid)은 2~3줄이었으며, 1 μm안에 10~14개가 있었다. 이러한 형태 특성은 10 μm 안에 있는 등줄안다리(fibulae) 수와 점무늬열(striae)의 수, 미소공(Poroid) 수에서 P. brasiliana와 크게 차이가 났다. 따라서 P. cf. brasiliana는 P. brasiliana의 변종이나 신종으로 추정된다. Several species of the genus Pseudo-nitzschia produce the neurotoxin domoic acid (DA) known to be responsible for amnesic shellfish poisoning. Recently off the coast of Geomundo and in Exclusive Economic Zone (EEZ) of Korea Strait, P. cf. brasiliana predominated in summer, 2010. As P. cf. brasiliana was new to Korea, we describe the morphological characteristics of the species. It was observed that cells were linear to lanceolate in valve and girdle view, 51~76 μm long and 1.9~2.6 μm wide. In the central part a large interspace was absent. The number of fibulae and striae in 10 μm were 30~37 and 31~37. Each stria contained 2~3 rows of poroids, with 10 to 14 poroids in 1 μm length. These morphological features of P. cf. brasiliana were found to be similar with the P. brasiliana, except with the fact that P. cf. brasiliana carred more numbers of fibulae/striae in 10 μm and poroids in 1 μm. Based on our observation, we suggest that P. cf. brasiliana is a newly identified Pseudo-nitzschia species or a variety of P. brasiliana.