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姜昌鎬,李鎭伊,柳晟基,朴俊哲 慶尙大學校生産技術硏究所 1999 生産技術硏究所論文集 Vol.15 No.-
This study deals with effects of surface treatment on the bending fatigue strength of SCM420 spur gears. We manufactured gears using manufacture processes that are currently used in most gears manufacturing companies, we applied heat-treatment, and then we applied shot peening. In addition, we investigated the effect these surface-treatments had on hardened layer and compression residual stress in the risk section of the teeth. The fatigue test at a constant stress amplitude is performed by using an electrohydraulic serve-controlled pulsating tester. The S-N curves are obtained and illustrated. We investigated the effect of surface-treatment on fatigue strength and the position of crack of gear teeth.
Characterization of a New β-Lactamase Gene from Isolates of Vibrio spp. in Korea
( Lyu Jin Jun ),( Jae Hoon Kim ),( Ji Woong Jin ),( Hyun Do Jeong ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.4
PCR was performed to analyze the β-lactamase genes carried by ampicillin-resistant Vibrio spp. strains isolated from marine environments in Korea between 2006 and 2009. All 36 strains tested showed negative results in PCR with the primers designed from the nucleotide sequences of various known β-lactamase genes. This prompted us to screen new β-lactamase genes. A novel β-lactamase gene was cloned from Vibrio alginolyticus KV3 isolated from the aquaculture water of Geoje Island of Korea. The determined nucleotide sequence (VAK-3 β-lactamase) revealed an open reading frame (ORF) of 852 bp, encoding a protein of 283 amino acids (aa), which displayed low homology to any other β-lactamase genes reported in public databases. The deduced 283 aa sequence of VAK-3, consisting of a 19 aa signal peptide and a 264 aa mature protein, contained highly conserved peptide segments specific to class A β-lactamases including the specific amino acid residues STFK (62-65), SDN (122-124), E (158), and RTG (226-228). Results from PCR performed with primers specific to the VAK-3 β-lactamase gene identified 3 of the 36 isolated strains as V. alginolyticus, Vibrio cholerae, and Photobacterium damselae subsp. damselae, indicating the utilization of various β-lactamase genes including unidentified ones in ampicillin-resistant Vibrio spp. strains from the marine environment. In a mating experiment, none of the isolates transfered the VAK-3 β-lactamase gene to the Escherichia coli recipient. This lack of mobility, and the presence of a chromosomal acyl-CoA flanking sequence upstream of the VAK-3 β- lactamase gene, led to the assumption that the location of this new β-lactamase gene was in the chromosome, rather than the mobile plasmid. Antibiotic susceptibility of VAK-3 β-lactamase was indicated by elevated levels of resistance to penicillins, but not to cephalosporins in the wild type and E. coli harboring recombinant plasmid pKV-3, compared with those of the host strain alone. Phylogenetic analysis showed that VAK-3 β-lactamase is a new and separate member of class A β-lactamases.
필리핀에서 유입된 Dengue hemorrhagic fever
배성진 ( Bae Seong Jin ),정진원 ( Jeong Jin Won ),김진영 ( Kim Jin Yeong ),김남중 ( Kim Nam Jung ),김양수 ( Kim Yang Su ),류지소 ( Lyu Ji So ),우준희 ( U Jun Hui ) 대한내과학회 2003 대한내과학회지 증례 특집호 65-5 부록3 Vol.0 No.-
Dengue Hemorrhagic Fever/dengue shock syndrome (DHF/DSS) is an acute febrile illness characterized by decreased platelet counts and hypovolemic shock due to increased vascular permeability and plasma leakage in patients infected with any one of four serot
Jin Sun Song,Dong Woon Lee,Suk Jun Lee,Chae Min Lee,Tae Heon Lim,Dongpyeo Lyu,Hyeong Hwan Kim,Myoung Rae Cho 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04
The green pale plant bug, Apolygus spinolae was one of the main insect pests that damaged leaves and fruit in grapes and its damage status was firstly reported in 2000 in grape orchards. This research was conducted to evaluate the distribution and difference in damage rate depending in management type of grapevine orchards (domestic sale farm vs export farm) in the export complex area of Korea (Hwangsung in Gyeonggii, Sangju and Yeongcheon in Gyeongbuk, Namwon in Junbuk and Yeongdong in Chungbuk) from 2010 to 2012. Damage by A. spinolae occurred in all 62 survey farms and damage rate differed depending on locality and individual farms in the same area. Damage rate was lower in export farms than in domestic sale farms, and damage rate of leaves was highly correlated with damage rate of new shoots. 15 species of hemipteran insect were attracted to sticky traps and A. spinolae was the dominant species. The attracted number of A. spinolae in the sticky traps differed depending on locality, and more occurred in domestic sale farms than expert farms. A. spinolae was continually attracted to sticky traps in the harvest period in grapevine orchards.