당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

Melanin-Concentrating Hormone Receptor 1 (MCHR1) Antagonists for the Treatment of Obesity

Yi, Kyu Yang 이화여자대학교 세포신호전달연구센터 2008 고사리 세포신호전달 심포지움 Vol. No.10

Obesity is gradually becoming a leading cause of morbidity as a result of an increase in associated risk factors such as dyslipidemia, type 2 diabetes, stroke, cardiovascular disease, and cancer. There are only two drugs currently approved in the United States for chronic treatment of obesity, Roche's Xenical(Orlistat), which blocks fat absorption from the gut, and Abbott's Meridia(Sibutramine), which is a mixed 5HT-NE uptake inhibitor. Recent pharmacological approaches to induce weight loss have included modulation of several metabolic process. One of the most promising new pharmacological targets in these endeavors is the melanin-concentrating hormone(MCH). MCH is a cyclic 19-amino acid peptide that is produced predominantly in neurons in the lateral hypothalamus and zona incerta. Several lines of evidence have shown that MCH-peptide plays a major role in body weight regulation in rodents: 1) Intracerebroventricular(icv) administration of MCH in rats stimulates food intake; 2) Chronic administration of the MCH peptide leads to increased body weight; and 3) Transgenic mice overexpressing the MCH gene are susceptible to insulin resistance and obesity; 4) Mice lacking the gene encoding MCH are hypophagic, lean, and maintain elevated metabolic rates; 5) MCHR1 null mice are lean, have decreased leptin and insulin levels, and are resistant to diet-induced obesity despite exhibiting a hypermetabolic phenotype. Small molecule antagonists of MCHR1 have been heavily pursued by many laboratories trying to find an effective anti-obesity agent. By November 2004, two MCHR1 antagonists(AMG-076, Amgen; GSK-856464, GlaxoSmithKline) had already entered Phase I clinical trials. However, no changes in status have been reported for either MCHR1 program to date. Several potent MCHR1 antagonists based on benzimidazole scaffolds have been designed, synthesized, and evaluated for the treatment of obesity. SAR analysis showed that some compounds were potent antagonists, with activities below 10 nM, which demonstrate very good oral bioavailability and other pharmacokinetic parameters in rats. Oral administration of the most potent compound in this class in the animal modelled to significant reduction of weight.

Bio-Frontier Symposium : Discovery of KR-33028, a novel Na+/H+ exchanger isoform-1 (NHE-1) inhibitor as a cardioprotective agent

( Kyu Yang Yi ),( Sun Kyung Lee ),( Byung Ho Lee ),( Sung Eun Yoo ) 한국생화학분자생물학회 (구 한국생화학회) 2006 생화학분자생물학회 춘계학술발표논문집 Vol.2006 No.-

Case Reports : Bilateral Necrotizing Fasciitis around the Hips Differentiated from Fournier Gangrene: A Case Report

( Bo Kyu Yang ),( Seung Rim Yi ),( Ye Hyun Lee ),( Hyun See Kim ),( Seok Woo Nam ),( Young Joon Ahn ),( Seong Wan Kim ),( Sung Wook Yang ),( Se Hyuk Im ) 대한고관절학회 2014 Hip and Pelvis Vol.26 No.4

As an emergency encountered in orthopedic practice requiring prompt diagnosis and aggressive treatment, necrotizing fasciitis around the hip must be discriminated from Fournier gangrene. The current case report describes a patient who suffered from bilateral type I necrotizing fasciitis around the hips, which was alleviated by prompt surgical debridement and intensive postoperative care.

C.B-17 SCID 마우스의 주령에 따른 기초연구 : 체중, 장기중량, 혈액학, 혈액생화학적 검사

최양규(Yang-Kyu Choi),류성주(Seong-Joo Lyu),현병화(Byung-Hwa Hyun),이철호(Chul-Ho Lee),윤원기(Won-Kee Yoon),정규식(Kyu-Shik Jeong),남윤이(Youn-Yi Nam),강부현(Boo-Hyon Kang),최재윤(Jae-Yoon Choi) 한국실험동물학회 1997 Laboratory Animal Research Vol.13 No.1

Detection of Irradiated Cereals by Viscosity Measurement

Yi, Sang-Duk,Chang, Kyu-Seob,Yang, Jae-Seung The Korean Society of Food Science and Nutrition 2000 Preventive Nutrition and Food Science Vol.5 No.2

A study was performed to establish the detection method of irradiated cereals. A drastic reduction of the apparent viscosity of suspensions with heat treatment was observed up to 1∼2 kGy in brown rice, Job's-tears, polished barley and polished rice. They were gently reduced to samples irradiated at 15 kGy. This trend was similar for all stirring speeds. The viscosity of unirradiated brown rice, Job's-tears, polished barley and polished rice reduced with in-creasing stirring speeds and this tendency was similar for irradiation doses. Regression expressions and coefficients of brown rice, Job's s-tears, polished barley and polished rice on different doses were 0.9399($y=3408.0e^{-0.2338x}$), 0.8855($y=3597.8e^{-0.6864x}$), 0.9343($y=7554.0e^{-0.4998x}$) and 0.9714($y=3228.2e^{-0.5312x}$), respectively, at 120 rpm. These results sug-gest that detection of irradiation for cereals could be possible by viscometric methods.

장관골에 발생한 피로골절의 자기공명영상 소견

양보규,한성호,정선욱,이승림,박양희,고동오 대한골절학회 2001 대한골절학회지 Vol.14 No.2

Purpose : The goal of our study was to evaluate diagnosis and management of stress fracture in long bones using MRI findings. Material & Method: Between May 1995 to May 1999, 40 patients( 45 cases ) were confirmed to have a stress fracture by clinical and radiological findings. All patients were evaluated with clinical, X-ray, bone scan, and MRI findings. The patient was 21 years in average( range from 18 to 23 years ). All were males and soldiers. The evaluation was made by comparison of MRI and plain radiograph, and duration of symptom was evaluated with MRI grading by Fredericson et al. Result: The locations of stress fracture of long bones were tibia(n=25), fibula(n=14), and femur(n=6). MRI findings were bone marrow edema in 38(84.4%)cases, intramedullary low signal intensity band in 19(42.2%)cases which was continuous with cortex and cortical fracture line. Periosteal reaction was seen in 45(100%)cases and surrounding soft tissue edema in 20(44.4%)cases. Plain X-ray findings were peristeal reaction in 31( 68.9%)cases, medullary sclerosis in 10(22.2%)cases, and cortical fracture line in 8(17.8%) cases. Duration of symptom was longer in higher MRI grade. Conclusion: MRI was more useful in early diagnosis and differential diagnosis of stress fracture, showing various findings than plain radiograph. MRI grading was helpful in planning tlhe therapy of stress fracture.

Identification of Irradiated Granule-Type Ramen Soup Powder by Pulsed Photostimulated Luminescence and Thermoluminescence during Storage

Yi, Sang-Duk,Yang, Jae-Seung,Kim, Dong-Woo,Shin, Doo-Ho,Jo, Gab-Yeon,Chang, Kyu-Seob,Oh, Man-Jin The Korean Society of Food Science and Nutrition 2003 Preventive Nutrition and Food Science Vol.8 No.1

This study was carried out to establish a method for determining if granule-type Ramen soup powder has been irradiated. Thermoluminescence (TL) and pulled photostimulated luminescence (PPSL) were used as the detection methods through observed changes of TL and PPSL intensities after storage under differing conditions. PPSL intensities increased with increases in irradiation doses. The threshold level of PPSL was below 412$\pm$58 photon counts regardless of storage conditions (room and darkroom) after 10 months. TL intensities also increased with increasing irradiation doses. The coefficients ($R^2$) of PPSL (0.74~0.94) and TL intensities (0.92~0.58) were very highly correlated with irradiation dose. The PPSL and TL intensities were decreased after 10 months of storage. These results indicate that discrimination of irradiated from non-irradiated granule-type Ramen soup powder is possible using TL and PPSL methods despite the decrease in intensities of TL and PPSL with increasing storage times.

Application of Viscometric Method for the Detection of Irradiated Black and White Pepper

Yi, Sang-Duk,Chang, Kyu-Seob,Yang, Jae-Seung 한국식품위생안전성학회 2000 한국식품위생안전성학회지 Vol.15 No.2

본 연구는 점도 측정법을 이용하여 방사선 조사된 검은 후추가루와 횐 후추가루의 조사유무를 확인하기 위해 이전의 연구에서 수행되지 않은 다양한 rpm에서의 검지 가능성과 회귀식 및 회귀계수를 설정하여 보다 최적화 되고 체계적인 검지 기술을 확립하기 위해 수행되어졌다 실험에 사용된 검은 후추가루와 흰 후추가루는 polyethylene bag에 포장되어졌고 한국원자력연구소내의 Co-60감마선 조사시설을 이용하여 2.5, 5, 7.5, 10, 15 kGy로 조사되어졌다. 10 %, 13%농도의 검은 후추가루와 7%, 10% 농도의 흰 후추가루 시료를 만들기 위하여 증류수를 가하여 현탁시킨 후에 알카리화 하였고 Brookfield DV-III rotation viscometer를 이용하여 30。C 에서 30초 동안 30. 60, 90, 120, 150, 180, 210 rpm에서 점도를 측정하였다. 모든 시료의 점도와 표준편차는 조사선량과 rpm이 증가할수록 감소하는 경향을 보였으며, 농도가 증가할수록 점도도 증가되어 농도 의존적인 경향을 보여 주었고, 이러한 경향은 모든 시료에서 유사하게 나타났다. 120 rpm 에서 10% black pepper, 13% black pepper, 7% white pepper, 10% white pepper 의 회귀식과 회귀계수는 0.9531 (y=-131.29x+1769.0), 0.9725 (y=-351.33x+4036.0), 0.9731 (y=2208.0e-${^-0.3546X}$), 및 0.9959 (Y=5116.0e${^-0.2887X}$)를 나타내었으며 조사선량과 점도간의 높은 상관성을 보여 주었다. 이상의 결과를 종합하여 볼 때 점도 측정법은 방사선 조사된 검은 후추가루와 흰 후추가루의 조사유무를 확인할 수 있는 유용한 방법임을 확인할 수 있었다. A study was carried out to establish a detection method for irradiated black and white pepper. Samples were packed in polyethylene bags and irradiated with 2.5, 5, 7.5, 10 and 15 kGy using a Co-60 irradiator. The samples were suspended in water, and alkalized with sodium hydroxide solution. Apparent viscosity was determined after heat gelatinization using a Brookfield DV-III rotation viscometer at 30。C with 30, 60, 90, 120, 150, 180, and 210 rpm. Means and standard deviations of the viscosities of all samples decreased by increasing the stirring speeds. The viscosities increased in all samples by increasing the concentration. Regression expressions and coefficients of viscosity which decreased with increasing irradiation dose of 10% and 13% black pepper, and 7% and 10% white pepper were 0.9531 (y=-131.29x+1,769.0), 0.9725 (y=-351.33x+4,036.0), 0.9731 (y=2,208.0e${^-0.3546X}$), and 0.9959 (y=5,116.0e${^-0.2887X}$), respectively, at 120 rpm. This trend was similar fur all stirring speeds. These results suggest that the detection of irradiated black and white pepper at various doses is possible by the viscometric method.

CdSe@ZnS/ZnS quantum dots loaded in polymeric micelles as a pH-triggerable targeting fluorescence imaging probe for detecting cerebral ischemic area

Yang, Hong Yu,Fu, Yan,Jang, Moon-Sun,Li, Yi,Yin, Wen Ping,Ahn, Tae Kyu,Lee, Jung Hee,Chae, Heeyeop,Lee, Doo Sung Elsevier 2017 Colloids and surfaces. B, Biointerfaces Vol.155 No.-

<P><B>Abstract</B></P> <P>High photoluminescence (PL) quantum yield (QY), photostability CdSe@ZnS/ZnS core/multishell quantum dots (CM-QDs) were first applied for bioimaging. The solubility, stability and biocompatible of the fluorescence imaging probes were constructed by self-assembly of CM-QDs and pH-responsive methoxy poly(ethylene glycol)-poly(β-amino ester/amidoamine)-dodecylamine (mPEG-PAEA-DDA) multiblock copolymers. The resulting CM-QDs-loaded mPEG-PAEA-DDA micelles (CM-QDs-PEG-PAEA-DDA) exhibited lower cell cytotoxicity and higher fluorescence intensity than the core/shell CdSe@ZnS QDs-encapsulated mPEG-PAEA-DDA micelles (CS-QDs-PEG-PAEA-DDA). Moreover, the in vivo fluorescence imaging ability confirmed that the CM-QDs-PEG-PAEA-DDA can be employed as a pH-triggerable targeting imaging probe for detection of a rat bearing cerebral ischemia disease. Therefore, we believed that the CM-QDs-PEG-PAEA-DDA may be the next generation of fluorescence imaging probes for targeted diagnosis acidic pathological areas, using pH as a stimulus.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CdSe@ZnS/ZnS QDs are synthesized and first applied for bioimaging. </LI> <LI> mPEG-PAEA-DDA copolymer showed pH-responsive property and the hydrolysis stability. </LI> <LI> CM-QDs-PEG-PAEA-DDA exhibited lower toxicity and higher fluorescent intensity. </LI> <LI> CM-QDs-PEG-PAEA-DDA has ability to target image for acidic brain ischemic area. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>