http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Jung, Jaejoon,Park, Woojun Springer-Verlag 2015 Applied microbiology and biotechnology Vol.99 No.6
<P>Acinetobacter occupies an important position in nature because of its ubiquitous presence in diverse environments such as soils, fresh water, oceans, sediments, and contaminated sites. Versatile metabolic characteristics allow species of this genus to catabolize a wide range of natural compounds, implying active participation in the nutrient cycle in the ecosystem. On the other hand, multi-drug-resistant Acinetobacter baumannii causing nosocomial infections with high mortality has been raising serious concerns in medicine. Due to the ecological and clinical importance of the genus, Acinetobacter was proposed as a model microorganism for environmental microbiological studies, pathogenicity tests, and industrial production of chemicals. For these reasons, Acinetobacter has attracted significant attention in scientific and biotechnological fields, but only limited research areas such as natural transformation and aromatic compound degradation have been intensively investigated, while important physiological characteristics including quorum sensing, motility, and stress response have been neglected. The aim of this review is to summarize the recent achievements in Acinetobacter research with a special focus on strain DR1 and to compare the similarities and differences between species or other genera. Research areas that require more attention in future research are also suggested.</P>
( Jaejoon Jung ),( Sang Jun Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.10
To adapt to environmental changes and to maintain cellular homeostasis, microorganisms adjust the intracellular concentrations of biochemical compounds, including metal ions; these are essential for the catalytic function of many enzymes in cells, but excessive amounts of essential metals and heavy metals cause cellular damage. Metal-responsive transcriptional regulators play pivotal roles in metal uptake, pumping out, sequestration, and oxidation or reduction to a less toxic status via regulating the expression of the detoxification-related genes. The sensory and regulatory functions of the metalloregulators have made them as attractive biological parts for synthetic biology, and the exceptional sensitivity and selectivity of metalloregulators toward metal ions have been used in heavy metal biosensors to cope with prevalent heavy metal contamination. Due to their importance, substantial efforts have been made to characterize heavy metal-responsive transcriptional regulators and to develop heavy metal-sensing biosensors. In this review, we summarize the biochemical data for the two major metalloregulator families, SmtB/ArsR and MerR, to describe their metal-binding sites, specific chelating chemistry, and conformational changes. Based on our understanding of the regulatory mechanisms, previously developed metal biosensors are examined to point out their limitations, such as high background noise and a lack of well-characterized biological parts. We discuss several strategies to improve the functionality of the metal biosensors, such as reducing the background noise and amplifying the output signal. From the perspective of making heavy metal biosensors, we suggest that the characterization of novel metalloregulators and the fabrication of exquisitely designed genetic circuits will be required.
Jung, Jaejoon,Park, Woojun American Society for Microbiology 2013 Applied and environmental microbiology Vol.79 No.20
<P><I>Alishewanella</I> species are expected to have high adaptability to diverse environments because they are isolated from different natural habitats. To investigate how the evolutionary history of <I>Alishewanella</I> species is reflected in their genomes, we performed comparative genomic and transcriptomic analyses of <I>A. jeotgali</I>, <I>A. aestuarii</I>, and <I>A. agri</I>, which were isolated from fermented seafood, tidal flat sediment, and soil, respectively. Genomic islands with variable GC contents indicated that invasion of prophage and transposition events occurred in <I>A. jeotgali</I> and <I>A. agri</I> but not in <I>A. aestuarii</I>. Habitat differentiation of <I>A. agri</I> from a marine environment to a terrestrial environment was proposed because the species-specific genes of <I>A. agri</I> were similar to those of soil bacteria, whereas those of <I>A. jeotgali</I> and <I>A. aestuarii</I> were more closely related to marine bacteria. Comparative transcriptomic analysis with pectin as a sole carbon source revealed different transcriptional responses in <I>Alishewanella</I> species, especially in oxidative stress-, methylglyoxal detoxification-, membrane maintenance-, and protease/chaperone activity-related genes. Transcriptomic and experimental data demonstrated that <I>A. agri</I> had a higher pectin degradation rate and more resistance to oxidative stress under pectin-amended conditions than the other 2 <I>Alishewanella</I> species. However, expression patterns of genes in the pectin metabolic pathway and of glyoxylate bypass genes were similar among all 3 <I>Alishewanella</I> species. Our comparative genomic and transcriptomic data revealed that <I>Alishewanella</I> species have evolved through horizontal gene transfer and habitat differentiation and that pectin degradation pathways in <I>Alishewanella</I> species are highly conserved, although stress responses of each <I>Alishewanella</I> species differed under pectin culture conditions.</P>
Jung, Jaejoon,Lee, Se Hee,Jin, Hyun Mi,Jeon, Che Ok,Park, Woojun Japan Society for Bioscience, Biotechnology, and A 2014 Bioscience, Biotechnology, and Biochemistry Vol.78 No.5
<P>Bacterial community and metabolites were analyzed in a flatfish jeotgal, a Korean fermented seafood. Inverse relationship of pH and 16S rRNA gene copy number was identified during fermentation. Lactobacillus was the predominant bacterial genus. Increase of Firmicutes was a common characteristic shared by other fermented seafood. Fructose, glucose, and maltose were the major metabolites.</P>
( Hyun Jung Kim ),( Hyo Jung Eom ),( Chulwoo Park ),( Jaejoon Jung ),( Bora Shin ),( Wook Kim ),( Namhyun Chung ),( In Geol Choi ),( Woojun Park ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.3
Microbially induced calcium carbonate precipitation (CCP) is a long-standing but re-emerging environmental engineering process for production of self-healing concrete, bioremediation, and long-term storage of CO2. CCP-capable bacteria, two Bacillus strains (JH3 and JH7) and one Sporosarcina strain (HYO08), were isolated from two samples of concrete and characterized phylogenetically. Calcium carbonate crystals precipitated by the three strains were morphologically distinct according to field emission scanning electron microscopy. Energy dispersive X-ray spectrometry mapping confirmed biomineralization via extracellular calcium carbonate production. The three strains differed in their physiological characteristics: growth at alkali pH and high NaCl concentrations, and urease activity. Sporosarcina sp. HYO08 and Bacillus sp. JH7 were more alkali- and halotolerant, respectively. Analysis of the community from the same concrete samples using barcoded pyrosequencing revealed that the relative abundance of Bacillus and Sporosarcina species was low, which indicated low culturability of other dominant bacteria. This study suggests that calcium carbonate crystals with different properties can be produced by various CCP-capable strains, and other novel isolates await discovery.
Lee, Jaejoon,Park, Eun-Jung,Noh, Jung Won,Hwang, Ji Won,Bae, Eun-Kyung,Ahn, Joong Kyong,Koh, Eun-Mi,Cha, Hoon-Suk Kluwer Academic/Plenum Publishers 2012 INFLAMMATION Vol.35 No.2
<P>The aim of this study is to compare the expression of TIM-3 from CD4+ T cells from rheumatoid arthritis (RA) patients and healthy controls and to evaluate the effect of galectin-9 (Gal-9) on apoptosis of CD4+ T cells in these patients. CD4+ T cells from RA patients and healthy controls were isolated from peripheral blood mononuclear cells and were activated. The expression of TIM-3 mRNA in CD4+ T cells was measured using real-time polymerase chain reaction. CD4+ T cells were activated in the presence of graded doses of Gal-9 or control, and Gal-9-induced cytotoxicity and apoptotic activity of CD4+ T cells were analyzed using MTT assays and annexin-V staining, respectively. TIM-3 mRNA expression was significantly lower in CD4+ T cells from RA patients compared with those in healthy controls (p???=???0.028). CD4+ T cell survival as measured by MTT assay when incubated with Gal-9 (15??nM) was significantly higher in RA patients than in healthy controls (p???=???0.002). Apoptotic activity of CD4+ T cells from healthy controls as measured by annexin staining increased with graded doses of Gal-9 (0??nM vs. 30??nM, 0??nM vs. 90??nM, p???=???0.016 each). However, apoptotic activity of CD4+ T cells from RA patients did not change despite the stimulation with Gal-9. Gal-9-mediated apoptosis of CD4+ T cells is dysfunctional in RA patients. Blunted Gal-9-mediated apoptosis may be exerted through underexpression of TIM-3 that negatively regulates Th1 response. Our data suggest that TIM-3 and its interaction with Gal-9 may play an important role in the pathogenesis of RA and may represent a potential therapeutic target.</P>
정재준(JaeJoon Jung),이창섭(Changseob Lee),이태희(TaeHee Lee) 대한기계학회 2003 대한기계학회 춘추학술대회 Vol.2003 No.11
Approximate optimization has become popular in engineering field such as MDO and Crash analysis<br/> which is time consuming. To accomplish efficient approximate optimization, accuracy of approximate model<br/> is very important. As surrogate model, Kriging have been widely used approximating highly nonlinear<br/> system . Because Kriging employs interpolation method, it is adequate for deterministic computer simulation.<br/> Because there are no random errors and measurement errors in deterministic computer simulation, instead of<br/> classical DOE ,space filling experiment design which fills uniformly design space should be applied. In this<br/> work, various space filling designs such as maximin distance design, maximum entropy design are reviewed.<br/> And new design improving maximum entropy design is suggested and compared.