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RISS 인기검색어
- 검색키워드
- 저자 : 황영미(Young Mi Whang) (검색결과 6 건)
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황영미,전길자,Whang, Young-Mi,Jhon, Gil-Ja 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.3
Methyl donor로 S-adenosyl-L-methionine을 사용해서 기질 단백질중 비정상 아미노산인 L-isoaspartyl residues의 ${\alpha}$-free carboxyl group을 methylester화 시키는 반응을 촉매하는 Protein Carboxyl Methyltransferase(PCM)를 소뇌에서 25%의 수득률로 약 1,000배 정제하였다. 소뇌의 cytosol을 70% ammonium sulfate fractionation, DEAE-cellulose chromatography, SAH-Sepharose 4B affinity chromatography 방법으로 정제하였으며, 정제된 효소용액을 HPLC (DEAE-5PW column)로 분석하여 3종류의 isoenzymes을 분리하였고 용출되는 순서에 따라 PCMI, PCMII, PCMIII으로 명명하였다. 더욱 정제된 효소용액으후 2차 전기영동한 결과 isoelectric point가 각각 6.25, 6.20, 5.80이었고 분자량은 모두 28,000이었다. 이 HPLC 분석방법은 소량의 사료로도 3개의 isoenzymes을 분리할 수 있는 장점을 지니고 있으므로 이 방법에 의해 비정상조직에서의 PCM isoenzymes 분포형태를 조사 할 수 있다. Protein carboxyl methyltransferase(S-adenosylmethionine: protein carboxyl O-methyltransferase, EC 2.1.1.77) of which methyl esterified L-isoaspartyl residues of protein substrate using S-adenosyl-L-methionine as a methyl donor has been purified from bovine brain approximately 1,000-fold with a yield of 25%. The enzymes were purified by following steps: 70% ammonium sulfate fractionation, DEAE-cellulose chromatography and SAH-sepharose 4B affinity chromatography. High performance liquid chromatography with anion-exchange column was utilized for the separation of protein carboxyl methyltransferase isoenzymes, and these enzymes were found to exist as three isoenzymes. The pI values of its isoenzymes were 6.25, 6.20, 5.80 respectively and the molecular weights of all isoenzymes were 28,000 by two-dimensional electrophoresis.
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진수빈(Subin Jin),황영미(Young Mi Whang),장인호(In Ho Chang) 대한비뇨기종양학회 2016 대한비뇨기종양학회지 Vol.14 No.2
With the emergence of microbiome as a major player in many human diseases, bacteria as therapeutics are gaining significant interest. Whole bacteria or cytotoxic or immunogenic peptides carried by them exert potent anti-tumor effects in the experimental models of cancer. The use of attenuated microorganism (s) e.g., BCG to treat human urinary bladder cancer was found to be superior compared to standard chemotherapy. While bacteria alone may not offer full therapeutic benefits, modifying them with anti-tumor agents, anti-oncogenes or immunogenic antigens, either alone or in combination, will prove to be beneficial. Vectors for delivering shRNAs that target oncogenic products, express tumor suppressor genes and immunogenic proteins have been developed. These approaches have showed promising anti-tumor activity in mouse models against various tumors. These can be potential therapeutics for humans in the future and such therapeutics may become a future alternative or adjunct regimen along with conventional chemotherapy and radiotherapy. In this review, some conceptual and practical issues on how to improve these agents for human applications are discussed.
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유전자 재조합 Bacille Calmette-Guérin을 이용한 비근육 침윤성 방광암의 면역요법
진수빈(Subin Jin),황영미(Young Mi Whang),장인호(In Ho Chang) 대한비뇨기종양학회 2016 대한비뇨기종양학회지 Vol.14 No.3
Intravesical instillation of Mycobacterium bovis bacille Calmette–Guérin (BCG) has been used for treating nonmuscle invasive bladder cancer as the forefront of immunotherapy, but BCG is ineffective in approximately 30–40% of cases and disease recurs in up to 50% of patients. Recently BCG is considered an effective vehicle for delivery of antigens due to its unique characteristics, and the genetic control of these mycobacteria is advanced in the search for less toxic and more potent therapeutic agents for bladder cancer immunotherapy. We will discuss current advances in recombinant BCG construction, research, and future directions.
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김명주(Myeong Joo Kim),진수빈(Subin Jin),황영미(Young Mi Whang),장인호(In Ho Chang) 대한비뇨기종양학회 2017 대한비뇨기종양학회지 Vol.15 No.1
There exists a need to develop strategies that promote neovascularization in virtually all tissue engineering and regenerative medicine efforts. While research typically focuses on understanding and exploiting the role of angiogenic factors and vascular cells on new blood vessel formation, the activity of the immune system is being recognized to impact vascular formation and adaptation. This review will provide both an overview of the relationship of angiogenesis and the immune system, and how biomaterials may be designed to promote favorable angiogenesis by interaction between these 2 systems to promote effective vascularization.
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김명주(Myeong Joo Kim),지병훈(Byung Hoon Chi),조민지(Min Ji Cho),황영미(Young Mi Whang),장인호(In Ho Chang) 대한비뇨기종양학회 2017 대한비뇨기종양학회지 Vol.15 No.2
Tissue engineering is limited by our inability to adequately vascularize tissues post implantation because all tissue-engineered substitutes (with the exception of cornea and cartilage) require a vascular network to provide the nutrient and oxygen supply needed for their survival. This review gives a brief overview of the processes and factors involved in the vascularization and angiogenesis and summarizes the different strategies to overcome the issue of slow vascularization and angiogenesis in a range of tissue-engineered substitutes. Moreover, we will announce some potential future plans.
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조민지(Min Ji Cho),지병훈(Byung Hoon Chi),김명주(Myeong Joo Kim),황영미(Young Mi Whang),장인호(In Ho Chang) 대한비뇨기종양학회 2018 대한비뇨기종양학회지 Vol.16 No.2
Three-dimensional (3D) printing is an additive manufacturing process by which precursor materials are deposited layer by layer to form complex 3D geometries from computer-aided designs, and bioprinting offers the ability to create 3D architecture living cells. Bioprinting methods have been developed rapidly pattern living cells, biological macromolecules, and biomaterials, and an advantage of the 3D microenviroment over traditional 2-dimensional cell culture is the ability to obtain more accurate and reliable data from model about tumor formation, progression, and response to anticancer therapies. This review focuses on recent advances in the use of biopriniting technologies for cancer research, bioprinting physiologically relevant testing platforms for anticancer drug development, and computational modeling for improvement bioprinting technique.
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