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홍용표,김규식,노의래,신은명,김진수,Hong, Yong-Pyo,Kim, Kyu-Sik,Noh, Eui-Rae,Shin, Eun-Myeong,Kim, Zin-Suh Korean Society of Forest Science 1998 한국산림과학회지 Vol.87 No.4
Portions of chloroplast genes(psbD and rbcL) were amplified from Pinus thunbergii(Japanese black pine : black pine) and Pinus densiflora(Japanese red pine : red pine) by PCR and digested by a restriction enzyme, HaeIII, respectively. Two species specific cpDNA markers were identified. With the observed cpDNA markers, paternal inheritance of cpDNA in pine hybrids was verified in an artificial hybrid family between black pine(Chollanam 37) and red pine(Chungchongbuk 3). On the basis of paternal inheritance of chloroplast genome in a hybrid, 2 portions of cpDNA amplified from 115 individuals of Pinus densiflora for. erecta were screened to detect any traces of black pine specific cpDNA markers in P. densiflora for. erecta which has been postulated as an introgressive hybrid between red pine and black pine(Hyun el al., 1967). All the analyzed individuals of Pinus densiflora for. erects revealed the identical profiles of HaeIII digested psbD and rbcL genes to red pine. This result suggests that there is no introduced chloroplast genome of black pine in Pinus densiflora for. erecta and that there is no concrete evidence of treating P. densiflora for, erecta as an introgressive hybrid between red pine(♀) and black pine(♂). 소나무와 해송으로부터 엽록체상의 두 유전자 psbD와 rbcL를 PCR에 의해 증폭한 후 제한효소 HaeIII를 사용해서 절단했다. 두 개의 종 특이적 엽록체 DNA 단편이 확인되었고, 이 두 개의 표지자를 이용하여 소나무(충북3호)와 해송(남난37호)의 인공교잡 가계로부터 엽록체 DNA의 부계 유전양식이 확인되었다. 인공교잡 가계에 있어서 엽록체 DNA의 부계 유전양식을 근거로 해송으로부터 소나무로의 이입교잡에 의해 생겨났다는 가설(현신규 등, 1967)이 지배적인 금강송 115개체로부터 이입교잡에 의해 유입되어진 흔적을 구명하기 위하여 해송 특이 엽록체 DNA의 존재 여부를 검색하였다. 분석에 사용된 금강송 전 개체에서 소나무에서 관찰된 엽록체 DNA(psbD와 rbeL)의 절편 분획 양상과 동일한 절편 분획 양상이 확인되었다. 본 실험의 결과로부터는, 금강송에는 해송으로부터 유입된 엽록체 게놈의 흔적을 찾아 볼 수 없었으며, 따라서 금강송을 소나무(♀)약 해송(♂)의 이입교잡종이이라고 간주할만한 확고한 증거를 제시할 수 없었다.
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소나무와 곰솔간 이입교잡종으로 추정되어온 금강송에 있어서 곰솔 cpDNA 의 부재
홍용표,김규식,노의래,신은명,김진수 ( Yong Pyo Hong,Kyu Sik Kim,Eui Rae Noh,Eun Myeong Shin,Zin Suh Kim ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.4
Portions of chloroplast genes(psbD and rbcL) were amplified from Pinus thunbergii(Japanese black pine : black pine) and Pinus densiflora(Japanese red pine : red pine) by PCR and digested by a restriction enzyme, HaeIII, respectively. Two species specific cpDNA markers were identified. With the observed cpDNA markers, paternal inheritance of cpDNA in pine hybrids was verified in an artificial hybrid family between black pine(Chollanam 37) and red pine(Chungchongbuk 3). On the basis of paternal inheritance of chloroplast genome in a hybrid. 2 portions of cpDNA amplified from 115 individuals of Pinus densiflora for. erecta were screened to detect any traces of black pine specific cpDNA markers in P densiflora for. erecta which has been postulated as an introgressive hybrid between red pine and black pine(Hyun el al., 1967). All the analyzed individuals of Pinus densiflora for, erects revealed the identical profiles of HaeIII digested psbD and rbcL genes to red pine. This result suggests that there is no introduced chloroplast genome of black pine in Pinus densiflora for. erecta and that there is no concrete evidence of treating P. densiflora for, erecta as an introgressive hybrid between red pine(♀) and black pine(♂).
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김진수,홍용표 ( Zin Suh Kim,Yong Pyo Hong ) 한국산림과학회 1982 한국산림과학회지 Vol.58 No.1
Megagametophyte and embryo tissue of Pinus densiflora were subjected to study the inheritance of glutamate-oxalate transaminase(GOT) and leucien aminopeptidase(LAP), and linkage relationship among isozyme loci coding both enzymes by starch gel zone-electrophoresis. Four zones of activity were observed for GOT. No variation was found in the fastest migrating zone (GOT-A), Electrophoretic phenotypes of the other two zones (GOT-B and GOT-C) showed 1:1 segregation ration, suggesting that each zone is controlled by a single locus. Foru and three alleles were identified at both loci respectively. The isozyme pattern of the fourth zone(GOT-D), migrated chthodally, coincided precisely with that of GOT-C, Whether the two zones are controlled by the smae locus or by two tightly linked loci remained unknown. In all three variant GOT zones, heterozygoes embryos produced triple band patterns, indicating that GOT isozyme in Pinus densiflora is a dimer. Two zones of activity stained for LAP were found. The segregation of the two zones (LAP-A and LAP-B) suggested that tow loci control each of both isozymes. Two and three alleles were identified at both loci. GOT-B and LAP-B were found to be tightly linked, showing an average recombination frequency of 12.5 percent. Slight deviation from independent assortment was observed between GOT-B and GOT-C, with recombination frequency of 41 percent.
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캘리포니아 폐구와 소나무류의 엽록체 DNA rbcL 과 atpB 유전자 사이에 존재하는 염기 서열
김진수,홍용표 한국유전학회 1992 Genes & Genomics Vol.14 No.2
The intergenic sequence between rbcL and atpB genes of chloroplast DNA were sequenced from five individual trees, one individual each of Monterey pine (Pinus radiata), knobcone pine (Pinus attenuata), and three geographic groups of bishop pine (Pinus muricata). No variation was observed among the 667 bp intergenic sequences of five individuals which had been directly sequenced with the PCR(polymerase chain reaction)-amplified DNA. Alignment of the upstream sequences (251 bp) of the rbcL gene with those of Douglas-fir showed about 88.4% similarity.
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