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흰쥐의 혈청 콜레스테롤 및 중성지방 수준에 미치는 인삼 총 사포닌의 영향
임창진,박은희,이동권,이송재,홍순근,Lim, Chang-Jin,Park, Eun-Hee,Rhee, Dong-Kwon,Lee, Song-Jae,Hong, Soon-Keun 생화학분자생물학회 1981 한국생화학회지 Vol.14 No.3
콜레스테롤을 과량 투여한 흰쥐에 정제된 인삼 총 사포닌을 용량별 및 경로 별로 투여하여 혈청 총 콜레스테롤 및 중성지방 수준에 마치는 영향을 검토하였다. 정상적인 흰쥐에서는 정제 총 사포닌의 투여 용량에 비례하여 혈청내 콜레스테롤이 감소되는 경향이 있었으며, 콜레스테롤을 과량 투여한 흰쥐에서는 총 사포닌 15 mg/kg을 경구 투여 함으로써 혈청내의 콜레스레롤양이 증가되었는데, 총사포닌 150 mg/kg을 경구투여 시에는 반대로 감소되었다. 콜레스테롤을 과량 투여한 흰쥐에 총 사포닌 15 mg 및 50 mg/kg을 복강내로 투여했을 때의 혈청내 콜레스테롤 함량은 같은 용량을 경구 투여했을 때의 콜레스테롤 함량과 비슷하였다. 콜레스테롤과 사포닌의 부분 가수분해 산물인 프로사포계닌 50 mg/kg을 경구 투여 했을 때의 혈청 콜레스테롤 수준은 콜레스테롤과 총 사포닌을 각각 500 mg/kg, 15 mg/kg을 동시에 경구 투여 및 복강내 주사 할 때와 유사하였다. 콜레스테롤과 총 사포닌을 동시에 투여한 흰쥐의 혈청내 중성지방수준은 콜레스테롤 단독 투여군에 비하여 다소 감소하였으나, 용량에 따른 차이는 인정되지 않았다. Effects of administration route and dose of ginseng total saponin on the levels of serum cholesterol and triglyceride in normal and cholesterol-administered rats were studied. The results are as follows: 1. In normal rats, serum cholesterol was decreased in proportion to the administered dose of purified total saponin. 2. In high-cholesterol administered rats, administration of total saponin 15 mg/kg (p.o.) increased serum cholesterol, but total saponin 150 mg/kg decreased it. And it was also observed that administration of prosapogenin 50 mg/kg had about the same effect as oral administration of total saponin 15 mg/kg. 3. Serum triglyceride was slightly decreased by administration of total saponin, but there was no correlationship between administered total saponin dose and serum triglyceride level.
흰쥐의 혈청 콜레스테롤 및 중성지방 수준에 미치는 인삼 총 사포닌의 영향
임창진,박은희,이동원,이송재,홍순근 ( Chang Jin Lim,Eun Hee Park,Dong Kwon Rhee,Song Jae Lee,Soon Keun Hong ) 생화학분자생물학회 1981 BMB Reports Vol.14 No.3
Effects of administration route and dose of ginseng total saponin on the levels of serum cholesterol and triglyceride in normal and cholesterol-administered rats were studied. The results are as follows 1. In normal rats, serum cholesterol was decreased in proportion to the administered dose of purified total saponin. 2. In high-cholesterol administered rats, administration of total saponin 15 ㎎/㎏ (p. o.) increased serum cholesterol, but total saponin 150 ㎎/㎏ decreased it. And it was also observed that administration of prosapogenin 50 ㎎/㎏ had about the same effect as oral administration of total saponin 15 ㎎/㎏. 3. Serum triglyceride was slightly decreased by administration of total saponin, but there was no correlationship between administered total saponin dose and serum triglyceride level.
주정흔 ( Jung Hun Ju ),임채형 ( Chae Hyung Lim ),김진호 ( Jin Ho Kim ),이정표 ( Jeong Pyo Lee ),손경희 ( Kyung Hee Son ),홍순근 ( Soon Keun Hong ),권태린 ( Tae Rin Kwon ),장미 ( Mi Chang ),김동섭 ( Dong Sup Kim ),윤혜성 ( Hae Seo 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1
Atopic dermatitis (AD) is a common pruritic disease and its incidence is increasing in infancy and childhood. AD is characterized by extreme pruritus and chronically relapsing inflammations and prevalence of AD has increased in recent years, but its exact etiology is unclear. Antigen-induced release of inflammatory mediators from mast cells causes the immediate symptoms of IgE-mediated allergic diseases, including allergic rhinitis, atopic dermatitis and atopic eczema. Mast cells rapidly release various allergic mediators, including histamine, cytokines that mediated acute and chronic allergic reaction. Since mast cells play a central role in the pathogenesis of various allergic diseases, we investigated the effect of exposure to 18 chemicals on the IgE-mediated allergic response in mast cells. We use two type of mast cells (RBL-2H3 cells, BMMC) to investigate the effects of chemicals on degranulation and cytokine production. Geraniol and formaldehyde significantly induced IgE-mediated mast cell degranulation. Dermatophagoides pteronyssinus allergen 1(DP) and dermatophagoides farinae allergen 1(DF) also induced degranulation without statistically significance. Also, we showed that release of IL-1β, IL-6 and GM-CSF was increased by treatment of geraniol. Also, We found that DP and DF induced release of IL-1β, IL-6, GM-CSF and TNF-α. These results suggest that in vitro method using mast cells degranulation and allergic cytokine secretion assay are useful for screening of atopic dermatitis trigger.
이동권(Dong Kwon Rhee),임창진(Chang Jin Lim),김두하(Doo Ha Kim),홍순근(Soon Keun Hong),박은희(Eun Hee Park),한용남(Yong Nam Han) 大韓藥學會 1982 약학회지 Vol.26 No.4
Acute toxicities of purified ginseng saponin (PGS) in mice, and subacute toxicities of PGS in rats were investigated. Average lethal doses (LD50) of PGS in male mice were 270mg/kg (i.v.), 342mg/kg (i.p.), 505mg/kg (i.m.), 950mg/kg (s.c.), and more than 5,000mg/kg (p.o.), respectively. Results of subacute toxicity of PGS was as follows. Body weight was markedly increased by administration of PGS 7.7mg/kg but side effects were shown by administration of 77mg/kg and above dose. Especially administration of PGS 240mg/kg caused a marked decrease of albumin/globulin ratio, and 28% increase of urea nitrogen in serum, as well as 33% increase of liver weight/body weight ratio.
연구논문 : 3T3 세포를 이용한 광독성 대체시험법 확립
주정흔 ( Jung Hun Ju ),이용경 ( Yong Kyoung Lee ),손경희 ( Kyung Hee Son ),이정표 ( Jeong Pyo Lee ),김진호 ( Jin Ho Kim ),임채형 ( Chae Hyung Lim ),홍순근 ( Soon Keun Hong ),권태린 ( Tae Rin Kwon ),장미 ( Mi Chang ),김동섭 ( Dong 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1
Phototoxicity is an acute toxic response induced by skin irradiation after the systemic or local administration of a chemical and subsequent exposure to light. Thus, it is necessary to evaluate phototoxicity of chemicals or products that are applied to the skin. Although the previous studies on the assessment of phototoxicity have been done using animals, it is necessary to establish alternative test methods, as EU ban animal-tested cosmetic ingredients or products. In vitro 3T3 NRU PT is an alternative method for identification of phototoxicity hazard chemicals as it compares cytotoxicity between UV irradiation and non-irradiation groups. Accordingly the accumulated information of phototoxicity assessment and its data has been found to be insufficient in Korea. Thus, we tested ten phototoxic and three non-phototoxic chemicals, and assessed two UV filter ingredients using in vitro 3T3 NRU PT mehtod. As a result, we identified that this method can be used to predict phototoxicity of chemicals. However, Eusolex 9020, UVA filter ingredient, was misclassified in vitro as a false positive compared with in vivo result. It suggests that other adequate test methods should be accompanied in order to enhance accuracy of phototoxicity prediction.
주정흔 ( Jung Hun Ju ),이용경 ( Yong Kyoung Lee ),손경희 ( Kyung Hee Son ),이정표 ( Jeong Pyo Lee ),김진호 ( Jin Ho Kim ),임채형 ( Chae Hyung Lim ),홍순근 ( Soon Keun Hong ),권태린 ( Tae Rin Kwon ),장미 ( Mi Chang ),김동섭 ( Dong 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1
Phototoxicity is an acute toxic response induced by skin irradiation after the systemic or local administration of a chemical and subsequent exposure to light. Thus, it is necessary to evaluate phototoxicity of chemicals or products that are applied to the skin. Although the previous studies on the assessment of phototoxicity have been done using animals, it is necessary to establish alternative test methods, as EU ban animal-tested cosmetic ingredients or products. In vitro 3T3 NRU PT is an alternative method for identification of phototoxicity hazard chemicals as it compares cytotoxicity between UV irradiation and non-irradiation groups. Accordingly the accumulated information of phototoxicity assessment and its data has been found to be insufficient in Korea. Thus, we tested ten phototoxic and three non-phototoxic chemicals, and assessed two UV filter ingredients using in vitro 3T3 NRU PT mehtod. As a result, we identified that this method can be used to predict phototoxicity of chemicals. However, Eusolex 9020, UVA filter ingredient, was misclassified in vitro as a false positive compared with in vivo result. It suggests that other adequate test methods should be accompanied in order to enhance accuracy of phototoxicity prediction.