가정 생활만족도의 개념정립을 위한 이론적 접근

홍성회 啓明大學校 生活科學硏究所 1994 科學論集 Vol.20 No.-

우수논문소개 : 배아줄기세포의 신경세포분화 연구 동향

홍성회,최경아 생화학분자생물학회(구 한국생화학분자생물학회) 2013 생화학분자생물학회 소식 Vol.33 No.1

식물 polyamine의 역할 및 대사 조절

신정섭,정지웅,홍성회 高麗大學校自然資源科學硏究所 1994 自然資源科學硏究 Vol.2 No.-

벼의 arginine decarboxylase DNA clone 의 재조합 및 염기서열 분석

홍성회(Sung Hoi Hong),신정섭(Jeong Sheop Shin),정지웅(Ji Ung Jeung),옥승한(Sung Han Ok) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.2

Arginine decarboxylase (ADC) is the first enzyme in one of the two pathways of diamine putrescine biosynthesis in plants. The genes encoding ADC have previously been cloned from Escherichia coli, oat and tomato genome. Two degenerate oligonucleotides (17-mer) corresponding to two conserved regions of ADC were used as primers in polymerase chain reaction of rice (Oryza sativa L.) genomic DNA, and an approximately 1.0 kbp fragment was obtained. This amplified PCR product showed an open reading frame which contains 1,022 by of nucleotide sequences. This PCR product was cloned into pGEM-originated T vector anti the short 500 by Pst1 digested fragment was subcloned into pGEM-3zf(+/-) vectors to facilitate sequencing. The nucleotide sequence of this PCR product showed about 74% and 70% identity with the same regions of the oat and tomato ADC cDNA sequences, respectively. The predicted .amino acid sequence exhibited 45% and 62% identity with oat and tomato ADC polypeptide fragments, respectively. The sequence similarities of 34%, 47%n and 38% were previously reported in oat and E. coli, tomato ;md oat, and tomato and E. coli ADC amino acids, respectively. Therefore, similarities and identities between rice and oat or tomato are remarkably higher than those others of the previous reports. In the highly conserved regions in both the amino acid sequence and spacing regions among the sequences of these three, rice ADC open reading frame also has the exactly same regions with the striking similarity. RNA blot analysis showed that ADC is expressed as a transcript of approximately 2.5 kbp in the rice seedling leaf tissues.

갑상선전절제시 술 후 저칼슘혈증 고위험군 환자에 대한 임상적 고찰

홍성회(Seong-hee Hong),허학준(Xuejun Xu),홍석준(Suk-Jun Hong) 대한외과학회 2001 Annals of Surgical Treatment and Research(ASRT) Vol.61 No.6

Prediction of personalized drugs based on genetic variations provided by DNA sequencing technologies

강성만,홍성회 한국유전학회 2011 Genes & Genomics Vol.33 No.6

Recent reports of death and illness caused by adverse drug reactions have boosted rational drug design research. It has been shown through sequencing of the entire human genome that human genetic variations play a key role in adverse reactions to drugs as well as in differences in the effectiveness of drug treatments. The advent of high-throughput DNA sequencing technologies with bioinformatics of system biology have allowed the easy identification of genetic variations and all other pharmacogenetic variants in a single assay, thus permitting truly personalized drug treatment. This would be particularly valuable for many patients with chronic diseases who must take many medications concurrently. In this review, we have focused on pharmacogenomics for the prediction of variable drug responses between individuals with relevant genetic variations through new DNA sequencing technologies and provided directions for personalized drug therapy in the future.

Molecular Pathogenesis of Spinocerebellar Ataxia Type 1 Disease

강성만,홍성회 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.6

Spinocerebellar ataxia type 1 (SCA1) is an autosomal-dominant neurodegenerative disorder characterized by ataxia and progressive motor deterioration. SCA1 is associated with an elongated polyglutamine tract in ataxin-1, the SCA1 gene product. As summarized in this review, recent studies have clarified the molecular mechanisms of SCA1 pathogenesis and provided direction for future therapeutic approaches. The nucleus is the subcellular site where misfolded mutant ataxin-1 acts to cause SCA1 disease in the cerebellum. The role of these nuclear aggregates is the subject of intensive study. Additional proteins have been identified, whose conformational alterations occurring through interactions with the polyglutamine tract itself or non-polyglutamine regions in ataxin-1 are the cause of SCA-1 cytotoxicity. Therapeutic hope comes from the observations concerning the reduction of nuclear aggregation and alleviation of the pathogenic phenotype by the application of potent inhibitors and RNA interference.

Motor neurons derived from ALS-related mouse iPS cells recapitulate pathological features of ALS

박주황,박항수,홍성회,강성만 생화학분자생물학회 2016 Experimental and molecular medicine Vol.48 No.-

Amyotrophic lateral sclerosis (ALS) is a late-onset progressive neurodegenerative disease characterized by the loss of motor neurons in the spinal cord and brain. Mutations in Cu/Zn superoxide dismutase 1 (SOD1) are known to induce ALS. Although many research models have been developed, the exact pathological mechanism of ALS remains unknown. The recently developed induced pluripotent stem (iPS) cell technology is expected to illuminate the pathological mechanisms and new means of treatment for neurodegenerative diseases. To determine the pathological mechanism of ALS, we generated mouse iPS (miPS) cells from experimental ALS transgenic mice and control mice and characterized the cells using molecular biological methods. The generated miPS cells expressed many pluripotent genes and differentiated into three germ layers in vitro and in vivo. Motor neurons derived from ALS-related miPS cells recapitulated the pathological features of ALS. The ALS-model motor neurons showed SOD1 aggregates, as well as decreased cell survival rate and neurite length compared with wild-type motor neurons. Our study will be helpful in revealing the mechanism of motor neuronal cell death in ALS.

Femtoliter Scale Quantitative Injection Control by Experimental and Theoretical Modeling

유제원,권효성,박항수,홍성회,최연호 대한의용생체공학회 2016 Biomedical Engineering Letters (BMEL) Vol.6 No.4

Purpose Injection is a useful technology for delivery thesolution like drug in medical science, because injectionamount can be simply controlled by adjusting the injectionduration and pressure. With advances in fabrication technology,the small size of syringe can be made from cm to nm scale. Therefore, its application model has been also decreased toa single cell. However, in femto-scale, the precise volumemeasurement and the theoretical estimation by the time andthe pressure becomes very challengeable, because of thedramatic change of inertial forces. In order to estimate thequantitative value of injected volume in femtoliter scale,empirical equation was established as injection pressure,duration and viscosity. Methods We observed the experimental results of theinjected volume in femtoliter scale as a function of viscosity,duration, and pressure. Based on these results, we design andexperimentally verify a theoretical equation for the quantitativecontrol of the volume in femto-scale. The quantitative deliveriesof the injection solution in a living cell were investigated. Results When the external material has viscosity between 80to 12 mPa·s, injection volume is observed to vary between22 to 46 fL. At same conditions, it was observed similaritiesbetween theoretical values and experimental results. Furthermore,fluorescent material was quantitatively injected into aliving single cell. The fluorescent intensity is linearly increasedwith the injection volume. Conclusions It is possible to quantitatively control theinjection volume using the empirical equation in the scaleof femtoliter without any process on coating an injection solution.

Thymidine decreases the DNA damage and apoptosis caused by tumor‐treating fields in cancer cell lines

Jeong Hyesun,Jo Yunhui,윤명근,홍성회 한국유전학회 2021 Genes & Genomics Vol.43 No.9

Background Tumor-treating felds (TTFields) is an emerging non-invasive cancer-treatment modality using alternating electric felds with low intensities and an intermediate range of frequency. TTFields afects an extensive range of charged and polarizable cellular factors known to be involved in cell division. However, it causes side-efects, such as DNA damage and apoptosis, in healthy cells. Objective To investigate whether thymidine can have an efect on the DNA damage and apoptosis, we arrested the cell cycle of human glioblastoma cells (U373) at G1/S phase by using thymidine and then exposed these cells to TTFields. Methods Cancer cell lines and normal cell (HaCaT) were arrested by thymidine double block method. Cells were seeded into the gap of between the insulated wires. The exposed in alternative electric felds at 120 kHz, 1.2 V/cm. They were counted the cell numbers and analyzed for cancer malignant such as colony formation, Annexin V/PI staining, γH2AX and RT-PCR. Results The colony-forming ability and DNA damage of the control cells without thymidine treatment were signifcantly decreased, and the expression levels of BRCA1, PCNA, CDC25C, and MAD2 were distinctly increased. Interestingly, however, cells treated with thymidine did not change the colony formation, apoptosis, DNA damage, or gene expression pattern. Conclusions These results demonstrated that thymidine can inhibit the TTFields-caused DNA damage and apoptosis, suggesting that combining TTFields and conventional treatments, such as chemotherapy, may enhance prognosis and decrease side efects compared with those of TTFields or conventional treatments alone.