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객담에서 분리한 Aspergillus 속의 RAPD를 이용한 분자생물학적 동정의 유용성
김영권 ( Young Kwon Kim ),홍성노 ( Sung Rho Hong ),김상하 ( Sang Ha Kim ),서충원 ( Choong Wonand Seo ) 대한임상검사과학회 2009 대한임상검사과학회지(KJCLS) Vol.41 No.4
On the basis of morphological characteristics, of total 128 strains of from sputum of tuberculos inpatient were identified as A. fumigatus (61 strains), A. niger (37), A. flavus (26), A. versicolor (1), A. nidulans (1), A. clavatus (1) and Neosartorya fennelliae (1). These strains were re-identified according to recent Aspergillus classification system which is mainly based on molecular characteristics. The strains were grouped by randomly amplified polymorphic DNA (RAPD) techniques. The representative strains from each group were sequenced with partial β-tubulin gene and compared with those of reference strains in the Aspergillus and were identified by the sequence. The identification was confirmed by morphology examination. As the results, they are reidentified as A. fumigatus (58), A. niger (11), A. tubingensis (26), A. flavus (27), A. sydowii (3), A. nidulans (1), A. clavatus (1) and Neosatorya fennelliae (1). This is the first report of A. tubuingensis in clinical field in Korea.