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Non-solvent induced phase separation을 이용한 삼중기공을 갖는 PCL scaffold 제작
이지은(Ji-Eun Lee),홍명화(Myoung Wha Hong),김희경(Hee-Kyeong Kim),조용상(Yong Sang Cho),김민수(Min-Soo Ghim),이세환(Se-Hwan Lee),김영율(Young Yul Kim),조영삼(Young-Sam Cho) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
In this study, PCL (Polycaprolactone) scaffold with triple pore was fabricated using non-solvent induced phase separation. First of all, PCL grains and PEO powder were dissolved in THF (Tetrahydrofuran). The slurry was mixed using hot plate for 24 hours at 40 ℃. Subsequently, PCL/PEO slurry was injected into the stainless steel barrel. Afterwards, to swap THF (solvent) with ethyl alcohol (non-solvent), the slurry was plotted at bed, which was filled with ethyl alcohol. Thereafter, the PCL/PEO structure was soaked in ethyl alcohol bed for 12 hours. After taking out the PCL/PEO structure from ethyl alcohol, to leaching out PEO particles, PCL/PEO structure was soaked in the D.I. water (Deionized water) for 12 hours. The obtained scaffold was dehydrated for 24 hours and stored in a vacuum desiccator. Consequently, hierarchical scaffold having interconnected pores was acquired using the bio-plotting. To assess the characteristics of fabricated scaffolds, morphological analysis and in-vitro cell-culturing were performed.
SLUP (Salt-Leaching Using Powder) 세포지지체의 생체 내외 거동 특성 평가
조용상(Yong Sang Cho),권미령(Quan Meiling),홍명화(Myoung Wha Hong),김영율(Young Yul Kim),조영삼(Young-Sam Cho) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.2015 No.11
In tissue engineering, salt-leaching method which is one of the conventional methods has been widely used, because if its easiness and simplicity. However, conventional salt-leaching method has some disadvantages: lack of interconnectivity and usage of solvent or high-pressure. Therefore, to overcome these drawbacks, our team developed SLUP (Salt-Leaching Using Powder) method which needs no solvent and high-pressure and provides good interconnectivity. In this study, proposed SLUP scaffold was compared to PED (Precision Extruding Deposition) scaffold using in-vivo and in-vitro experiments. Firstly, SLUP scaffold with ratio of 1:5 wt % (PCL/NaCl) was prepared. Secondly, PED scaffold was prepared with 500 micrometer strand size and 1000 micrometer distance of strands. After that, to assess cell-culture characteristics of osteoblast, scaffolds were assessed by in-vitro CCK-8 assay for 14 days. Furthermore, for in-vivo experiments, rats having calvaria defect were used.
2D 형상을 갖는 Polydioxanone 세포지지체에서 공극크기에 따른 세포배양 특성평가
조아라(A Ra Jo),지민희(Min Hee Jee),정훈진(Hoon Jin Jung),홍명화(Myoung Wha Hong),윤지혜(Ji Hye Yun),김영율(Young-Yul Kim),조영삼(Young-Sam Cho) 대한기계학회 2012 대한기계학회 춘추학술대회 Vol.2012 No.11
In this study, cell-culturing characteristics are assessed with several different types of two dimensional polydioxanone(PDO) scaffolds. Firstly, 110㎛ and 230㎛-diameter PDO monofilaments are prepared. Secondly, PDO monofilaments with different diameter, respectively, are laid orthogonally having different pore sizes. On the other hand, two-dimension scaffolds, which having different pore sizes, are knitted using PDO monofilaments with different diameter, respectively. After that, adipose stem cell, adipose stem cell with Polydeoxyribonucleotide(PDRN), and adipose stem cell with platelet-rich plasma(PRP) are seeded on scaffolds to assess the cell-culturing characteristics.