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티오아세트아미드(thioacetamide) 복강투여로 인한 무지개송어, Oncorhynchus mykiss의 간장 및 신장 독성 반응 연구
허민도 ( Min Do Huh ),정다혜 ( Da Hye Jeong ) 한국어병학회 2023 한국어병학회지 Vol.36 No.2
In veterinary medicine for mammals, studies are being conducted to confirm the effects of antioxidants using pathological toxicity model studies, and are also used to confirm the effect of mitigating liver or kidney toxicity of specific substances. It was considered necessary to study such a toxicity model for domestic farmed fish, so thioacetamide (TAA), a toxic substance that causes tissue damage by mitochondrial dysfunction, was injected into rainbow trout (Oncorhynchus mykiss), a major farmed freshwater fish species in Korea. The experiment was conducted with 40 rainbow trout (Oncorhynchus mykiss) weighting 53 ± 0.6 g divided into two groups. Thioacetamide(TAA) 300mg/kg of body weight was intraperitoneally injected into rainbow trout and samples were taken 1, 3, 5, 7 days after peritoneal injection. As a result, in serum biochemical analysis, AST levels related to liver function decreased 3 and 5 days after intraperitoneal injection and increased after 7 days, and ALT levels also increased after 7 days. In addition, creatinine related to renal malfunction increased 3 and 5 days after TAA injection. In histopathological analysis, pericholangitis and local lymphocyte infiltration were observed in the liver from 1 day after intraperitoneal injection of TAA, and hepatic parenchymal cell necrosis was also observed from 3 days after intraperitoneal injection. Hyaline droplet in renal tubular epithelial cell was observed from 1 day after TAA injection, and acute tubular damage such as tubular epithelial cell necrosis appeared from 3 days after TAA injection. Accordingly, it is thought that it will be able to contribute to studies that require a toxicity model.
정현도,허민도 ( Hyun Do Jeong,Min Do Huh ) 한국수산과학회 1995 한국수산과학회지 Vol.28 No.1
Defining the mechanisms of B cell diversification which establish the immune repertoire is fundamental to understand how the immune response is regulated. In this report, B cell differentiation and diversification focused on the regulation of immunoglobulin V_(H) gene expression during ontogeny were analyzed by in situ hybridization technique. Fetal liver B cells in different gestational days from 16d to 20d showed the predominant expression of V_(H)7183 and V_(H)Q52 without transition of repertoire during the observed gestation days. The two subsets of fetal liver B cells separated according to different differentiation stages based on the presence of cell surface immunoglobulin also did not indicate apparent difference in expressed V_(H) gene family profiles. B cells in fetal spleen as an another hematopoietic lymphoid tissue in fetus also expressed similar V_(H) gene repertoire to that in fetal liver B cells. This distinct pattern of V_(H) gene expression in fetal B cells from that of adult B cells were not changed even after four weeks contact with adult bone marrow microenvironment supplied by the established adult bone marrow stromal cell layers. Thus, the restricted V_(H) gene repertoire of B cells in fetus which is distinct from that in adult appears to be associated more with the genetic potential of fetal B cell progenitors and less with environmental influences or differentiation stages or compartmentalization.
Enterovirus 감염 돼지의 간에서 virus의 형태학적 관찰
신태균,이차수,허민도,Shin, Tae-kyun,Lee, Cha-soo,Huh, Min-do The Korean Society of Veterinary Science 1992 大韓獸醫學會誌 Vol.32 No.2
Enterovirus감염 돼지의 감염초기 virus의 동태를 규명하고자 초유를 섭취하지 않는 1~2일령의 돼지에 돼지 enterovirus 3형을 경구감염시킨후 간조직을 전자현미경으로 관찰하였던 바 다음과 같은 결과를 얻었다. 감염후 3일째 부검한 돼지의 간에서 간세포의 심한 공포변성이 인정되었고 간 동양혈관 내피세포 및 Kupffer세포에서 picornavirus의 특징적인 virus결정이 출현하였으며 확장된 ER은 virus 결정과 밀접하게 관련되어 있었다. 또한 동양혈관 세포와 간세포에서는 미숙한 virus모양의 입자들로 구성된 세포질내 봉입체가 관찰되기도 하였다. 이상의 소견으로 보아 돼지 enterovirus 3형은 신경친화성 뿐만 아니라 감염초기 간에 친화성이 큰 것으로 생각된다. Oral infection of colostrum-deprived, neonatal piglets with porcine enterovirus serotype 3 can result in hepatic lesions with a short incubation period. In the thin section of the affected liver, crystalline arrays of virus particles characteristic of picornavirus were identified in the sinusoid endothelial cells and Kupffer cells. There were also cytoplasmic aggregates of electron- dense, virus-like particles in the hepatocytes. These findings suggest that porcine enterovirus serotype 3 has hepatotropism as well as neurotropism.
저수온기 양식 조피볼락, Sebastes schlegeli 간에 대한 조직병리학적 연구
최혜승 ( Hye Sung Choi ),허민도 ( Min Do Huh ),이무근 ( Mu Kun Lee ),최희정 ( Hee Jung Choi ),박명애 ( Myung Ae Park ) 한국어병학회 2011 한국어병학회지 Vol.24 No.3
This study was aimed to determine the fish physical status according to the gross and histopathological findings of liver in cultured black rock fish, Sebastes schlegeli. All 47 fish submitted had no marked abnormalities in the external findings. 42.55% of fish showed normal liver, 25.53% yellow liver, 25.53% atrophic brown liver, 4.26% yellowish-green liver and 2.13% fatty liver in gross examination. Grossly normal liver showed no remarkable change in lobular structure but many vacuoles were found in hepatic cell. Hepatic cells took normal roundish, polygonal shapes containing spherical nuclei. In group of yellow-brown liver, many brown pigments were seen in hepatic cells, MMCs and brown-colored hyaline droplets within cytoplasm of hepatocytes. Yellowish green pigments were seen in hepatic cells and MMCs of yellow green liver and green colored hyaline droplets within hepatocytes. The dilated central veins are highlighted with atrophy of hepatic cells. Outline of atrophic hepatocyte became ambiguous and nucleus frequently become small and pyknotic. Fatty liver showed prominent vacuolar structures in cells as clear spaces or foamy cytoplasm with degenerative nuclei. From these results, it was strongly suggested that hepatic gross and histological findings could be used as important and critical health parameters of fish prior to progression to substantial manifestation as clinical disease.
하재이(Jai Yi HA),손상규(Sang Gyu SOHN),허민도(Min Do HUH),정현도(Hyun Do JEONG) 한국수산과학회 1996 한국수산과학회지 Vol.29 No.5
To avoid the self-agglutination of Staphylococcus aureus sensitized with rabbit antibody in the absence of antigen, we determined the optimum concentration of rabbit antibody for sensitization. It was analyzed by using three different kinds of S. aureus strains at various concentraions of antibody. The optimized coagglutination test using the S. aureus sensitized with rabbit antibody was applied to the diagnosis of edwardsiellosis in field and in laboratory. The presence of E. tarda as low as 10 ㎍/㎖ was detected by this method. Moreover, it showed good coagglutination results against several different forms of antigens such as FKC, EDTA or heat extracted antigen of E. tarda. E. tarda strains, isolated from the flounders suffering from edwardsiellosis in fields, showed some cross-reactions to the E. tarda 219 analyzed by both agglutination and coagglutination test with rabbit anti-E. farda 219 antibody. The degree of cross-reactions analyzed was enough to apply the coagglutination test for the diagnosis of edwardsiellosis in field. Thus, even 1,000 fold diluted tissue homogenate of infected flounder naturally contained enough E. tarda as an antigen to show good coagglutination with S. aueus sensitized with rabbit anti-E. tarda 219 antibody. The successful application of this method to the homogenate and heat extract of tissues from naturally or artificially infected flounder or tilapia proved that coagglutination test was a simple and rapid reliable dignostic technique suitable for using in laboratory and field without any special equipments.