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구기자나무(Lycium chinense Mill.) 유식물체의 자엽하배축으로부터 고효율의 부정아 형성과 식물체 재생
조만현,함인기,이봉춘,김재훈,이원석,권석윤,이행순,곽상수,Jo, Man-Hyun,Ham, In-Ki,Lee, Bong-Chun,Kim, Jae-Whune,Lee, Won-Seok,Kwon, Suk-Yoon,Lee, Haeng-Soon,Kwak, Sang-Soo 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.3
두 품종 (청양재래, 명안)의 구기자나무 (Lycium chinense Mill.) 유식물체를 자엽하배축의 자엽 일부분만 남기고 절단 후, 절단면이 1.0 mg/L zeatin이 첨가된 MS배지에 접하도록 하여 암소에서 3주 배양한 후 명소로 옮겨 배양하였을 때 자엽하배축 절단면으로부터 부정아가 고빈도 (80% 이상)로 유도되었다. 그러나 자엽하배축절편을 암소에서 전 배양하지 않고, 명소에서만 배양 하였을 경우 부정아는 유도되지 않았다. 자엽하배축 절단면에서 유도된 부정아를 분리하여 1.0 mg/L IAA가 함유된 발근배지에서 4주간 배양하여 뿌리를 유도하였다. 잎과 뿌리가 잘 발달한 소식물체는 화분에 옮겨 키웠을 때 정상적인 형태의 재분화 식물체로 성장하였다. To establish high frequency shoot formation from two cultivars (Cheongyangjaerae and Myungan) of boxthorn (Lycium chinense Mill.), hypocotyl segments with cotyledons from seedlings were used as explants. High frequency adventitious shoot formation (more than 80%) were obtained from hypocotyl segments with cotyledon on MS medium supplemented with 1.0 mg/L zeatin, when precultured for 3 weeks under dark conditions followed by transfer to light conditions. But there was no shoot induction in the explants cultured without preculture under dark conditions. Roots were induced from the shoots when transferred to rooting medium supplemented with 1.0 mg/L IAA for 4 weeks. Regenerated plantlets were grown to normal mature plants in soil.
Components in Chinese Cabbage (Brassica rapa ssp. campestris) as Affected by Soil pH: 6.9 vs. 7.6
Man Hyun Jo(조만현),In Ki Ham(함인기),Eun Mo Lee(이은모),Jo-Eun Lee(이조은),Nan Jiang(강남),Yong Pyo Lim(임용표),Gilhwan An(안길환) 충남대학교 농업과학연구소 2010 농업과학연구 Vol.37 No.1
Functional and nutritional components of 7 cultivars of Chinese cabbage (CC; Brassica rapa subspecies campestris) from China were analyzed to compare the effects of soil pH (6.9 vs. 7.6). The CC grew on the soil of pH6.9 (CC-6.9) contained significantly increased amounts (2.3~4-fold) of pectin, crude protein, vitamin C and vitamin E compared to the control. The contents of ash and minerals (Ca, Fe, Na, Zn, K and Mn) were also significantly increased in CC-6.9. However, CC-6.9 contained 16-75% lower contents of reducing sugars, cellulose and crude fat than CC-7.6. CC-7.6 contained more glucosinolates than CC-6.9; gluconasturtiin (20.68 vs. 0.84 nmolㆍg?¹ wet wt) and gluconapin (202.55 vs. 0.15 nmolㆍg?¹ wet wt). In conclusion, CC-6.9 had an improved texture (high pectin and low cellulose) and nutritional value (high in protein, Ca, Fe, Zn, vitamin C, and E), whereas CC-7.6 had better taste (high in reducing sugars) and anticancer functionality (high in glucosinolates).