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글리세롤 기반의 바이오에탄올 생산을 위한 연속생산반응기의 성능 비교
이상준(Sang Jun Lee),송윤석(Yoon Seok Song),김성봉(Sung Bong Kim),강성우(Sung Woo Kang),한성옥(Sung Ok Han),박철환(Chulhwan Park),김승욱(Seung Wook Kim) 한국생물공학회 2011 KSBB Journal Vol.26 No.4
Ethanol production using glycerol as a carbon source was performed by Enterobacter aerogenes immobilized on calcium alginate beads. To improve the ethanol production, the optimal conditions such as loading amount of immobilized cells and glycerol concentration were investigated. The optimal loading amount of immobilized cells and glycerol concentration were 10 mL of calcium alginate bead and 10 g/L, respectively. Consequently, glycerol consumption rate, ethanol concentration and yield were 0.32 g/L · h, 3.38 g/L and 0.43 g/g on the batch production, respectively. Continuous production of ethanol was successfully achieved using two types of immobilized cell reactors (continuous stirred tank reactor and packed bed reactor) from 10 g/L of glycerol. In the continuous stirred tank reactor, glycerol consumption, ethanol concentration, specific productivity and yield were 9.8 g, 4.67 g/L, 1.17 g/L · h, 0.48 g/g, respectively. The concentration of produced ethanol was 38-44% higher comparison to batch fermentation, and continuous stirred tank reactor showed better performance than packed bed reactor.
Enterobacter aerogenes를 이용한 crude glycerol 기반의 바이오에탄올 생산
정홍섭(Hongsub Jung),성필제(Pil-Je Seong),고아라(A-Ra Go),이상준(Sang Jun Lee),김승욱(Seung Wook Kim),한성옥(Sung Ok Han),조재훈(Jaehoon Cho),조대행(Dae Haeng Cho),김용환(Yong Hwan Kim),박철환(Chulhwan Park) 한국생물공학회 2011 KSBB Journal Vol.26 No.3
The effects of pH, glycerol concentration and salt on cell growth and ethanol production using Enterobacter aerogenes KCTC 2190 were evaluated in the anaerobic culture condition. In condition of initial pH 5, cell growth and ethanol production were highest. An initial concentration of 10 g/L of pure glycerol gave the highest cell growth and ethanol production. However, in case of over 15 g/L of pure glycerol, they decreased. The cell growth and ethanol production decreased with the increase of salt concentration. When 10 g/L of crude glycerol was used as the carbon source, the cell growth and ethanol production were 1.32 OD600 and 3.95 g/L, respectively, which were about 94.4% and 88.5% compared to those of pure glycerol. These result indicates that the crude glycerol produced in the biodiesel manufacturing process may be useful as a potential carbon source for ethanol production form Enterobacter aerogenes KCTC 2190.
생물화공 : Phanerochaete chrysosporium 변이주에서의 Cellobiose Dehydrogenase(CDH)와 β-Glucosidase 활성 향상
김은지 ( Eun Ji Kim ),강성우 ( Seong Woo Kang ),송광호 ( Kwang Ho Song ),한성옥 ( Sung Ok Han ),김재진 ( Jae Jin Kim ),김승욱 ( Seung Wook Kim ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.1
Hemoflavoenzyme으로서 cellobiose dehydrogenase(CDH)는 셀룰로오스를 분해하는 과정에서 세포 외부로 분비되는 효소로서 amorphous cellulose와 강하게 결합하여 셀룰라아제(cellulase)에 의해 microcrystalline cellulose의 가수분해를 증가시킨다. 따라서 CDH는 바이오 에탄올 생산의 당화공정에서 중요한 역할을 할 것으로 예상된다. 여러 백색부후균으로부터 CDH 생산이 높은 Phanerochaete chrysosporium ATCC 32629 균주를 선정하였으며, 균주로부터 생산된 CDH 효소활성의 최적 온도와 pH는 각각 55 °C와 4이었다. CDH 활성을 증가시키기 위하여 P. chrysosporium ATCC 32629 균주를 돌연변이시켰다. 돌연변이는 새로운 시도로써 국부적으로 큰 에너지를 줄 수 있는 특징을 가진 양성자 빔을 이 용하였다. 양성자 빔 조사 후 사멸율이 약 99.9%인 1.2 kGy에서 CDH 활성이 증가된 변이주를 얻었다. 선별된 변이 주와 모균주를 액체배양했을 때 변이주가 모균주보다 CDH와 β-glucosidase 활성이 각각 약 1.4배와 20배 증가하였다. 따라서, CDH 뿐만 아니라 β-glucosidase 활성이 높은 P. chrysosporium 변이주를 확보하였다. Cellobiose dehydrogenase(CDH) as a hemoflavoenzyme is secreted out of cell in the cellulose degradation. As CDH strongly bound to amorphous cellulose, it helps cellulose hydrolysis by cellulase. CDH may have an important role of saccharification process for bioethanol production. In this study, Phanerochaete chrysosporium ATCC 32629 was selected for the production of CDH among other strains tested. The optimal temperature and pH of CDH produced by P. chrysosporium ATCC 32629 were 55 °C and 4, respectively. To improve the activity of CDH, the mutation of P. chrysosporium was performed using proton beam that has high energy level partially. As a result, P. chrysosporium mutant with the high activity was selected at 1.2 kGy in a range of 99.9"10 lethal rate. The CDH and P-glucosidase activities of mutant were 1.4 fold and 20 fold higher than those of wild strain. Therefore, P. chrysosporium mutant with the high activities of CDH and j3-glucosidase was obtained from mutation by proton beam irradiation.