Comamonas terrigena의 균체지방산 조성

하덕모,안병학 한국미생물학회 1987 미생물학회지 Vol.25 No.1

Cellular fatty acid composition of eight strains, indluding six strains of Comamonas terrigena, and two type strains of Pseudomonas acidovorans, and P. testosteroni was determined by gas-liquid chromatography. Almost the same composition was found in all the strains tested, and hexadecanoic acid, hexadecenoic acid, and octadecenoic acid were accounted more than 70% of total fatty acid. However, P. testosteroni differed from C. terrigena and P. acidovorans by the presence of comparatively large amonuts of 2-hydroxy-hexadecanoic acid, and C. terrigena contained three to eight times as much tetradecanoic acid in P. acidovorans and P. testosteroni. According to the similarity values calculated on the basis of fatty acid composition, C. terrigena strains were divided into three groups differentiated in the requirement of growth factors, and C. terrigena, P. acidovorans, and P. testosteroni strains occupied separate position each other in the dendrogram.

Pseudomonas 屬細菌의 電氣 泳動 pattern

河德模 동국대학교 1982 論文集 Vol.21 No.-

好氣性, Gram陰性, 桿菌인 Pseudomonas 屬細菌은 大端히 廣範圍한 有機化合物을 分解資化하지만 그 多樣性 때문에 菌種間의 相互關係가 分明하지 않는 部分이 많고 또 分離菌株의 同定도 어려울때가 있어서 一般的인 分類나 同定의 경우보다 많은 種類의 炭素化合物에 대한 資化性을 檢討하게 된다. 近來 分類學에 있어서는 生化學的性質이나 表現形質에 의한 從來의 方法以外에 GC含量, 細胞壁組成, 菌體脂質, DNA-DNA 交雜등 化學分類學的인 方法에 의한 硏究가 이루어지고 있으며 Pseudomonas 屬細胞에 대해서도 菌體脂肪酸組成에 의해서 菌種相互關係를 밝히고 同定에도 利用될 수 있다는 것이 報告^1)되고 있고 또 r-RNA-DNA 交雜에 의해서 菌種間의 類緣關係를 硏究한 報告^2)도 있다. 菌體酵素의 電氣泳動 pattern은 DNA가 反映된 것의 하나로 생각할 수 있으므로 化學分類의 手法으로 硏究되고 있으며 Bacillus^3), Mycobacterium^4), Pasteurella^5), Enterobacteriaceae 科^6), Gram 陰聲 메탄올資化性細菌^7, 8, 9)등에 대한 硏究에서 電氣泳動이 分類에 있어서 大端히 效率的인 方法이라는 것이 報告되고 있다. 著者는 Pseudomonas 屬細菌의 代表的인 菌株에 대해서 各種酵素의 電氣泳動 pattern을 比較하고 菌種間의 相互關係에 대해서 檢討하였으므로 報告하고자 한다. Pseudomonas 屬細菌의 代表的인 18 菌株에 대해서 glutamate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, fumarase, catalase 및 glutamate-oxaloacetate transaminase의 菌體酵素 電氣泳動 pattern을 比較하여 다음과 같은 結果를 얻었다. 1) 供試菌株中 P. testosteroni KS 0043 및 P. diminuta KS 0007를 除外한 各菌株는 各各 다른 電氣泳動 pattern을 나타내었다. 2) Acidovorans group의 菌株는 fumarase 活性 主 band의 Rm 値가 같으며 이들菌株中 P. testosteroni KS 0043, P .diminuta KS 0006 및 P. diminuta KS 0007는 GDH를 除外한 各酵素活性 主 band의 Rm 値가 모두 같으므로 서로 가까운 類緣關係에 있으며 P. testosteroni KS 0043과 같은 pattern을 나타내는 P. diminuta KS 0007는 P. testosteroni의 synonym으로 推定하였다. 3) Fluorescent group 및 achromogenic group 以外의 菌株에서는 GDH 活性 band를 檢出할 수 없으며 P. maltophilia 以外의 菌株에서는 GOT 活性 band가 極히 弱하거나 檢出할 수 없었다. 4) 各菌株間의 電氣泳動 pattern의 差異는 菌種 도는 菌株間의 差異를 나타내며 電氣泳動 pattern의 比較는 菌種間의 類緣關係를 空明하는데 效果的인 方法으로 생각된다. 끝으로 本硏究를 積極的으로 도와주신 日本 東京大學 應用微生物硏究所 駒形和男敎授에게 깊히 感謝하는 바이다. (本論文의 要旨는 日本農藝化學會大會에서 講演하였다.) Extracts fom 18 strains representing Pseudomonas species were examined for each of 6 enzyme activities(glutamate dehydrogenase, malate dehydrogenass, citrateiso dehydrogenase, fumarase, catalase and glutamate-oxaloacetate transaminase) by polyacrylamide slab gel electrophoresis. All strains exhibited unique patterns of enzyme migration for each species, except the strains of acidovorans group which resembled to each other in enzyme migration patterns. The strains of acidovorans group seemed to be very closely related to each other phylogenetically. Among these strains, P. dacunhae Ks 0007 is presumed as the synonym of P. testosteroni KS 0043 due to the same electrophoretic enzyme types. The bands of activity of glutamate dehydrogenase were detected only in the species of fluorescent group and chromogenic group, and the intense band for glutamateoxaloacetate transaminase only in the strains of P. maltophilia. The unique banding patterns found in several species indicate that this technique may serve as a valuable aid to existing taxonomic schemes.

酵母의 榮養要求에 關한 硏究

河德模 東國大學校 1971 論文集 Vol.8-9 No.-

The growth requirements of Sacharomyces carlsbergensis A. T. C. C. 9080 in the mdeia containing various nitrogen sources are investigated. The results are as follows : 1) The yeast requires pyridoxine, biotin and Ca-pantothenate for the growth in the medium containing L-glutamic acid as the sole source of nitrogen. On the other hand, the yeast requires pyridoxine and biotin but does not Ca-pantothenate in the medium containing(NH_4)_2SO_4 as the solesource of nitrogen. 2) The yeast grows remarkably in the complete medium containing DL-alanine, L-arginlne, L-aspartic acid, L-glutamic acid, L- leucine, DL-serine, DL-valine and (NH_4)_2SO_4 respectively as the sole source of nitrogen. The yeast grows considerably without Ca-pantothenate in the medium containing DL-metionine, DL-phenylalanine, DL-serine and (NH_4)_2SO_4 as the sole source of nitrogen. 3) When Ca-pantothenate is deficient, the growth of the yeatsis remarkably inhibited by addition iof L-cystine, L-glutamic acid, L-histidine, DL-isoleucine, L-lysine, DL-tryprophan and DL-valine respectively in the medium in which (NH_4)_2SO_4 is the sole source of nitrogen and inhibited by addition of L-lysine and L-cystine respectively in the medium in which L-aspartic acid is the sole sole source of nitrogen. Being added the various amino acids to the media, the medium containing (NH_4)_2SO_4 is given different inhibition effects on the growth from the medium containing L-aspartic acid.

學會事務室의 移轉

河德模 한국식품과학회 1976 식품과학과 산업 Vol.9 No.2

글루타민酸 소오다의 投與가 人體의 血液 및 尿성분에 미치는 영향

河德模,辛孝善 東國大學校 1974 論文集 Vol.13 No.-

This study was designed to investigate the change of chemical composition of the serum and urine in humans after the MSG administration. Healthy Korean adult men were used for experimental objects. The experimental groups were divided into three groups receiving, one, three and five gram dosages, respectively. These groups were investigated as to the change of chemical composition in the serum and urine 2 and 24 hours after the administration. These findings were compared with the pre-administration condition. The results obtained are summarized as follows: 1) Among all experimental groups, the pH value, the non-protein nitrogen and the protein nitrogen content in the serum showed no marked changes after the administration, as compared to before the administration. 2) Among all experimental groups, the ammonia-nitrogen content in the serum, as compared to before the administration, decreased 2 hours after the administration but the urea-nitrogen content increased. 3) among all experimental groups, the blood sugar content in the serum increased slightly 2 hours after the administration, but the cotnent returned to the same level as before the administration. 4) Among all experimental groups, the albumin, α_1-, α_2- and γ-globulin content in the serum protein after the administration, as compared to before the administration, showed no market changes each time but the β-globulin content increased slightly after the administration. 5) Among all experimental groups, the urea-nitrogen and ammonia-nitrogen content in the urine after the administration, as compared to before the administration, increased slightly but the creatine and creatinine content showed no marked changes each time.

酵母의 胞子形成에 미치는 窒素源의 影響

河德模 東國大學校 1967 論文集 Vol.3-4 No.-

The effects of various nitrogen sources on ascus formation were investigated. 1) The addition of 1% peptone was the most effective on ascus formation amongthe media added (NH_4)_2SO_4, NaNO_3, and peptone to Koji extract, respectively. 2) DL-aspartic acid, L-cystine, L-glutamic acid, L-glycine, L-isoleucine, DL-serine, DL-tryptophan, and L-tyrosine stimulated the ascus formation, and α-alanine, L-arginine, DL-phenylalanine, and L-asparagine stimulated the growth in media in which amino acid is the sole source of nitrogen. 3) The effects of addition of amino acid to Koji extract medium on ascus formation are similar as the results in media in which amino acid is the sole source of nitrogen. 4) The mixture of effective amino acids on ascus formation stimulated the formation remarkably, but the stimulatory effects of amino acids were abolished by addition of α-alanine and L-arginine. These results indicate that-α-alanine and L-arginine inhibit the ascus formation.

酵母의 胞子形成에 미치는 Vitamine의 影響

河德模 東國大學校 1968 論文集 Vol.5 No.-

The effects of Vitamines on the sporulation of Schizosaccharomyces octosporus on the medium containing one of amino acids or casamino acid as the sole source of nitrogen was investigated. The results are as follows: The percentage of cells producing spores is appreciably increased on the medium containing inositol and Ca-pantothenate. Whereas, when nocotinic acid and biotin are present, the percentage is decreased. No such effects are observed when thiamine riboflavin, pridoxine, nicotinic acid, biotin, folic acid, and p-aminobenzoic acid are added. The yeast required inesitol, Ca-pantothenate, nocotinic acid, and biotin for growth. These results indicate that inositol and Ca-pantothenate are effective on both of sporulation and growth, nicotinic and biotin inhibit sporulation and stimulate only growth.

Aspergillus melleus가 生成하는 抗生物質에 對한 硏究

河德模 동국대학교 농림과학연구소 1969 農林科學 論文集 Vol.3 No.-

1) Asperigillus melleus produces the antibiotic which shown antibiotic activity against the pellicle forming yeast of soy, so called Shiro-Kabi. 2) By using stationary culture of Asp. melleus in the medium containing wheat bran extract and sucrose, the antibiotic substance was obtained. The crude antibiotic was separated from the culture liquor as crystal form by ether extraction at pH 9-11. 3) The antibiotic was identified as mellein. 4) Mellein inhibits the growth of Shiro-Kabi at concentration of 0.03% and inhibits the growth of yeasts, bacteria, and molds at concentrations of 0.05 to 0.1%.

[卷頭言] 韓國食品料學會와 産學協同

河德模 한국식품과학회 1986 식품과학과 산업 Vol.19 No.1

醱酵法에 의한 酢酸으로부터의 글루타민산소오다 製造

河德模 한국산업미생물학회 1974 한국미생물·생명공학회지 Vol.2 No.1