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종이크로마토그래피에서 거름종이의 다공성 및 입자 머무름과 분리능과의 상관관계
표명화,김성규 晋州敎育大學校 科學敎育硏究所 2001 科學敎育硏究 Vol.27 No.-
The Purpose of this study was to separate of qualitative analysis toward the mixture of aqueous pen using chromatography in the unit the separation of the mixture in 4th grade elementary school science. Paper chromatography is basically a type of partition chromatography between water adsorbed onto the cellulose fiber of the paper and a liquid mobile phase in a closed tank. The most common application is the ascending solvent technique and circular developing methods. The paper is hung by means of clips or string and the lower end is made to dip into the eluting solvent. The porosity, adsorption and developing velocity were to investigate of filter paper and paper chromatogiaphy was to separate of a mixture aqueous color pen from using ascending of strips and circular paper chromatography of filter paper. The results are as following: First, Adsorption and development velocity depends upon porosity and particle retention of using filter paper. Second, First of all resolving power is good using chromato-strip that water is best in single developing solvents system and water/acetone (9:1) solvents is best in mixing solvents system using chromato-strip. Third, The resolving power of chromato-strip are very good on porosity and particle retention of filter paper and chromatography papers(2.5㎛>8㎛>11㎛>l㎜>3㎜>20-25㎛). Also the resolving power of using circular paper chromatography gave the same results (2.5/㎛>8㎛>11㎛>l㎜>3㎜>20-25㎛). I want to suggest to a pillar of a paper circular paper chromatography are suited for a short and a coarseness filter paper. The use of thick paper such as filter paper (6㎛), paper chromatography (3㎜) are available in principle similar to thin layer chromatography when used in the ascending method.