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최홍서(Hong-Seo Choi),임송원(Song-won Lim),조성만(Sung-man Cho),강예진(Ye-Jin Kang),김민주(Min-Ju Kim),이정민(Jung-Min Lee),박구만(Goo-Man Park) 대한전자공학회 2018 대한전자공학회 학술대회 Vol.2018 No.6
Deep learning is used in various fields, especially the YOLO algorithm, which is one of the many deep-running frameworks, has very fast speed and accuracy that does not lag behind other frameworks. This paper includes a central control system for detecting and recognizing vehicle license plate using YOLO, identifying the vehicle type of the parked vehicle on the parking lot, and checking the parking position of each vehicle in the parking lot based on the recognized car number We intend to facilitate comprehensive parking vehicle management.
Bacillus thuringiensis BT-1, BT-2가 생산하는 δ-endotoxin의 특성 규명
김영민,최홍서,정건섭,Kim, Young-Min,Choi, Hong-Seo,Chung, Kun-Sub 한국생명과학회 2007 생명과학회지 Vol.17 No.5
B. thuringiensis는 많은 해로운 곤충들을 박멸시키는데 널리 사용되는 생물학적 살충제로 잘 알려져 있다. 그것은 측포자 형태의 결정체($\delta$-내독소)를 생산하고 내생포자들을 형성한다. 본 논문에서는 B. thuringiensis BT-1과 BT-2에 의해 생산되는 내독소의 특성을 주사전자현미경(SEM), 투과전자현미경(TEM), SDS-PACE, 알칼리 용액에서의 용해 활성을 통하여 규명하였다. BT-1, BT-2 균주는 GBY 배지에서 배양되었고, 두 균주의 내독소는 당밀도구배법을 이용하여 정제되어, 주사전자현미경(SEM), 투과전자현미경(TEM)으로 관찰하였다. 형태학적으로, BT-1의 내독소는 사각형 및 납작형이고 크기는 $1.73{\mu}m{\times}0.7{\mu}m$, 그리고 BT-2 내독소는 구형이며 $1.1{\mu}m$, 폭이 $0.9{\mu}m$인 것으로 밝혀졌다. SDS-PACE 방법으로 분석한 결과, BT-1의 분자량은 28 kDa, 21 kDa, 반면에 BT-2의 분자량은 50 kDa, 35 kDa, 22 kDal으로 밴드가 형성되었다. 이들의 결정체는 알칼리 완충용액 내에서 시간에 지나감에 따라서 점차로 용해되었으며 3시간 후에는 거의 완전하게 용해되었다. 이 결과들을 통하여 BT1과 BT-2 결정체의 내독소가 곤충의 중장 내에서 시간이 흐름에 따라 비활성 상태에서 활성상태로 전환되는 것으로 보여진다. Bacillus thuringiensis is a well-known species of entomophathogenic bacteria that is widely used as a biopesticide against many insect pests. It produces parasporal crystals ($\delta$-endotoxin) and endospores during sporulation. In this report, the $\delta$-endotoxin produced by Bacillus thuringiensis BT-1 and BT-2 were characterized by Scanning Electron Microscope(SEM), Transmission Electron Microscope(TEM), SDS-PACE, and solubilization activity by alkaline solution. BT-1, BT-2 were cultured in the GBY medium, and the $\delta$-endotoxin of them was purified with discontinuous sucrose density gradient centrifugation. Their $\delta$-endotoxin was observed by SEM and TEM. Morphologically, the $\delta$-endotoxin of BT-1 was a square and flat type, whose size was $1.73{\mu}m{\times}0.7{\mu}m$, and the $\delta$-endotoxin of the BT-2 was spherical form whose size was $1.1{\mu}m{\times}0.9{\mu}m$ determined by SEM and TEM. The $\delta$-endotoxin of the BT-1 was composed of 28 kDa and 21 kDa, however, it of the BT-2 was composed of 50 kDa, 35 kDa, and 22 kDa bands determined by SDS-PACE. The purified crystals of BT-1 and BT-2 were dissolved gradually in alkaline solution as time goes by, and it was perfectly dissolved after 3 hours. It is supposed that the $\delta$-endotoxin of crystal was converted to a state of activation in the course of time in the intestines of insect.
영상 대비 개선 알고리즘 비교와 성능 개선을 위한 알고리즘 연구
전소연(So-Yeon Jeon),최홍서(Hong-Seo Choi),이홍종(Hong-Jong Lee),박구만(Goo-Man Park) 대한전자공학회 2017 대한전자공학회 학술대회 Vol.2017 No.6
In this paper, adaptive algorithm considering the loss of color and brightness on images is used to improve the performance of existing image contrast enhancement algorithm. The improved algorithm is proposed by combining existing algorithms, color balance and tone mapping. We performed experiment comparing histograms of result images by subjective evaluation. The experiment result showed that the proposed algorithm reduced the loss of color and brightness on the images.