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수계 중금속의 미생물학적 처리 - 균주 개량에 관한 연구 -
이희준,최영길 ( Heu Joon Lee,Young Keel Choi ) 한국환경생물학회 1993 환경생물 : 환경생물학회지 Vol.11 No.2
Two strains of yeasts, HYM 40 and HYM 20 which have resistance against heavy metals such as cadmium and lead and have ability to accumulate the heavy metals were isolated in the Han river and industrial sewage. These yeasts were identified as Torulopsis inconspicua. These strains can accumulate lead up to 180㎎/g dried cell and cadmium up to 147.5㎎/g dried cell. In the electron microscopic analysis, it was found that these heavy metals were in the cell wall. In order to compare the wild types with the amino acid auxotroph mutants in accumulation ratio, in case of cadmium, mutant M-3 accumulated 43.65% after 2 hours incubation and wild type did 31.64% after 4 hours incubation. On the other hand, mutant M-3 accumulated lead up to 79.18% and wild type did up to 37.22% after 2 hours, 4 hours incubation, respectively. On the basis of these results, when M-2 and M-3 were treated with 40% of PEG and 30mM of CaCl_2 for protoplast fusion, F-l accumulated cadmium up to 46.81% within 4 hours and lead up to 85. 63% within 2 hours. These results represent improvement of heavy metal accumulating abilities. When compared with original strain, this fusant shows 46% of increase rate in case of cadmium and 118% in case of lead.
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최용,최영길,이희준,안연준 漢陽大學校 環境科學硏究所 1994 環境科學論文集 Vol.15 No.-
한강수계인 안양천에서 유출되는 산업페수에서 카드뮴과 납에 내성을 지니며 이들 중금속을 축적하는 균주 HYM 2., HYM 40을 분리하여 동정한 효모인 Torulopsis inconspicua로 밝혀졌다. 납의 경우 180mg/g dried cell, 카드뮴의 경우 147.5mg/g dried cell까지 축적한는 것을 확인하였다. 전자현미경으로 관찰한 결과 이들 중금속은 주로 세포막에 축적되는 것으로 나타났다. 이 균주를 한양학원 폐수를 재료로 하여 pilot system에 적용한 결과 원폐수만을 적용했을 때보다는 활성슬러지를 첨가했을 때 (MLSS를 2000∼3000mg/ㅣ로 보정), 특히 분리된 균주를 적용시켰을 때 중금속의 제거 효율이 훨씬 더 증가하였다. 이는 환경오염원을 제어하는 균주를 직접 실공정에 적용할 수 있는 가의 가능성을 타진할 수 있었다. Two strains of yeasts, HYM 20 and HYM 40, which have resistance against heavy metals such as cadmium and lead and have ability to accumulate the heavy metal were isolated in the industrial sewage drained from Anyang stream. These strains of yeasts were identified as Torulopsis inconspicua. This yeast accumulated lead upto 180mg/g dried cell and cadmium 147.5mg/g dried cell. In the electron microscopic analysis, it was observed that the heavy metals were accumulated within the cell membrane. In the application of wastewater from Hanyang University to the A₂/0 system, increased accumulation was observed when the original sewage was added with active slvge by comparison with the sewage alone. particulaly, when added with sluge and isolated strains, higher increasemant of accumulation was boserved than in the former cases. These results showed the possibilities for the practical elimination of heavy metal pollutants.
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