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UVB 손상 세포에 대한 열처리 소나무 유래 추출물의 항산화 및 항염증 효과
정지영 ( Ji Young Jung ),하시영 ( Si Young Ha ),이동환 ( Dong Hwan Lee ),최민서 ( Min Seo Choe ),양재경 ( Jae-Kyung Yang ) 한국목재공학회 2021 한국목재공학회 학술발표논문집 Vol.2021 No.1
Oxidative stress and chronic inflammatory processes are major risk factors for aging and age-related diseases. The present study investigated the antioxidant and anti-inflammatory effects of extracts derived from steam-exploded pine in UVB-exposed human keratinocytes, HaCaT cells. The effects of extracts derived from steam-exploded pine on oxidative stress were examined in a UVB-induced aging model of human keratinocyte HaCaT cells. Extracts derived from steam-exploded pine protected cells from UVB-induced cytotoxicity (80 μg/mL) and reduced reactive oxygen species (ROS) production by UVB irradiation. Expression of genes associated with antioxidant effects, including superoxide dismutase 1 (SOD1) and catalase(CAT), increased in a dose-dependent manner. The expression of interleukin 6 (IL6), interleukin 6 (IL7), interleukin 8 (IL8), macrophage inflammatory protein-1β (MIP-1β), and monocyte chemoattractant Protein-1 (MCP-1) genes, which are associated with inflammation, also decreased in a dose-dependent manner. These results suggest that the extracts derived from steam-exploded pine may have an inhibitory effect on oxidative stress and inflammation.
동충하초 균사체 및 동충하초 균사체로부터 분리된 코디세핀의 항암 활성 평가
하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),이동환 ( Dong Hwan Lee ),최민서 ( Min Seo Choe ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2021 한국목재공학회 학술발표논문집 Vol.2021 No.1
Various medicinal fungi have been traditionally used in many countries for health maintenance, as well as for the prevention and treatment of various diseases. Dong-Chong-Xia-Cha in Chinese, which translates as “winter worm and summer grass”, is an entomogenous fungus that colonized the larvae or pupae of insect. Although various biological activities of crude extracts have been reported, the precise active compounds responsible for the anticancer activities of cultured Cordyceps are not well defined. Therefore, as part of our study program to identify potential anticancer agents from traditional medicines, we used cordycepin isolated from Cordyceps militaris obtained by submerged cultivation. The aim was to isolate the pure cordycepin from the extracts of Cordyceps militaris and evaluate its anticancer properties. Silica gel column chromatographic purification of Cordyceps militaris extracts resulted in the isolation of cordycepin. The isolated cordycepin and crude cordyceps were examined for their anti-proliferative effects on human cancer cell lines, HCT, MC5-7, U-87MG, AGS, A549, and MCF-7 cells were analyzed. The isolated cordycepin displayed potent growth inhibition on HCT, MC5-7, U-87MG, AGS, A549, and MCF-7 cells with IC50 values of 86.16, 28.13, 282.40, 78.41, 137.40, and 216.40 μg/ml, respectively. A similar inhibitory trend in these cancer cells was observed for crude cordyceps, with IC50 values ranging from 60.8 317.2. The present study provides scientific supporting information for the ethnopharmacological use of Cordyceps militaris as an anticancer agent.
붉은사슴뿔버섯으로부터 유방암세포에 항암활성을 가지는 roridin E의 함량 증대를 위한 조건 배양
이동환 ( Dong Hwan Lee ),하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),최민서 ( Min Seo Choe ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2021 한국목재공학회 학술발표논문집 Vol.2021 No.1
Roridin E is a trichothecene that can be obtained from a poisonous mushroom known as Podostroma cornu-damae. Roridin E has anticancer activity against breast cancer cell lines; thus, it can be used to treat breast cancer. P. cornu-damae mycelia were cultured in submerged culture to obtain roridin E. By comparing the inside and outside of the mycelium, it was confirmed that more roridin E was present in the culture broth outside the mycelium. Variables were determined using the one-factor-at-a-time method. Response surface methodology (RSM), based on a three-level, three-variable Box-Behnken design (BBD), was employed to obtain an ideal combination of initial pH, incubation time, and rotational speed to roridin E production. The optimum culture conditions were as follows : initial pH as 4,; incubation time, 12.9 d; and rotational speed, 63 rpm. Under these conditions, the predicted production was 40.26 mg/L. The separated roridin E was found to have anti-cancer activity in five breast cancer cell lines: MCF-7, MDA-MB-231, HCC-70, T47D, and BT-474. The IC50 values were found to be 0.0004 - 0.002 mg/L.
Zanthoxylum schinifolium에서 추출한 hydrosol이 항 알레르기 염증에 미치는 영향
하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),이동환 ( Dong Hwan Lee ),최민서 ( Min Seo Choe ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2021 한국목재공학회 학술발표논문집 Vol.2021 No.1
Zanthoxylum schinifolium is a deciduous shrub, which grows abundantly in Korea, China, Taiwan, Manchuria, and Japan. Currently, still most of Z. schinifolium is being extracted with essential oils, and then chemical components are analyzed. Further, various pharmacological effects have been studied. However, the yield of oil separated from the fruit or leaves of Zanthoxylum is significantly low (below 10%). Hydrosol, also called hydrolate or oral water, is a hydrophilic fraction that can be obtained during the essential oil extraction process as a byproduct. It has a pleasant aroma and is recognized for commercial applications. However, limited reports are available on this aspect of Z. schinifolium. This study investigated for the first time the analysis by GC/MS and the potential anti-inflammatory activities of hydrosol, specifically those of the branch from Z. schinifolium. A total of 102 components were identified in the hydrosol of the branch. The main chemical components of the hydrosol were estragole (50.86 %) and camphor (30.68 %). Our findings showed that hydrosol obtained from branches inhibited β-hexosaminidase release in a dose-dependent manner in RBL-2H3 mast cells, and confirmed that hydrosol at the tested concentrations did not show cytotoxicity to RBL-2H3 cells by MTT assay. Additionally, we found that hydrosol obtained from the branches significantly inhibited intracellular active oxygen (ROS) and nitric oxide (NO). Consistently, the SNARE protein of SNAP23, syntaxin4, VAMP7, and VAMP8 were also remarkably decreased by hydrosol treatment. Further investigation identified camphor and estragole, the main chemical components of hydrosol, to downregulate LPS-induced phosphorylation of the SNARE protein. Based on these findings, hydrosol obtained from the branch of Z. schinifolium is suggested to have therapeutic potential for allergic inflammatory diseases.