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RISS 인기검색어
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- 저자 : 최문정(Mun-Jeoung Choi) (검색결과 2 건)
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배양된 치유두 유래세포의 조골활성 및 골기질 형성의 평가
박봉욱,변준호,최문정,하영술,김덕룡,조영철,성일용,김종렬,Park, Bong-Wook,Byun, June-Ho,Choi, Mun-Jeoung,Hah, Young-Sool,Kim, Deok-Ryong,Cho, Yeong-Cheol,Sung, Iel-Yong,Kim, Jong-Ryoul 대한악안면성형재건외과학회 2007 Maxillofacial Plastic Reconstructive Surgery Vol.29 No.4
In the present study, we focused on stem cells in the dental papilla of the tooth germ. The tooth germ, sometimes called the tooth bud, is the primordial structure from which a tooth is formed. The tooth germ consists of the enamel organ, the dental papilla, and the dental follicle. The dental papilla lies below a cellular aggregation of the enamel organ. Mesenchymal cells within the dental papilla are responsible for formation of dentin and pulp of a tooth. Tooth germ disappears as a tooth is formed, but that of a third molar stays in the jawbone of a human until the age of 10 to 16, because third molars grow slowly. Impacted third molar tooth germs from young adults are sometimes extracted for orthodontic treatment. In the present study, we evaluated the osteogenic activity and mineralization of cultured human dental papilla-derived cells. Dental papillas were harvested from mandible during surgical extraction of lower impacted third molar from 3 patients aged 13-15 years. After passage 3, the dental papilla-derived cells were trypsinized and subsequently suspended in the osteogenic induction DMEM medium supplemented with 10% fetal bovine serum, 50 g/ml L-ascorbic acid 2-phosphate, 10 nM dexamethasone and 10 mM -glycerophosphate at a density of $1\;{\times}10^6\;cells/dish$ in a 100-mm culture dish. The dental papilla-derived cells were then cultured for 6 weeks and the medium was changes every 3 days during the incubation period. Dental papilla-derived cells showed positive alkaline phosphatase (ALP) staining during 42 days of culture period. The formation of ALP stain showed its maximal manifestation at day 7 of culture period, then decreased in intensity during the culture period. ALP mRNA level was largely elevated at 1 weeks and gradually decreased with culture time. Osteocalcin mRNA expression appeared at day 14 in culture, after that its expression continuously increased in a time-dependent manner up to day 28. The expression remained constant thereafter. Runx2 expression appeared at day 7 with no detection thereafter. Von Kossa-positive mineralization nodules were first present at day 14 in culture followed by an increased number of positive nodules during the entire duration of the culture period. Osteocalcin secretion was detectable in the culture medium from 1 week. The secretion of osteocalcin from dental papilla-derived cells into the medium greatly increased after 3 weeks although it showed a shallow increase by then. In conclusion, our study showed that cultured human dental papilla-derived cells differentiated into active osteoblastic cells that were involved in synthesis of bone matrix and the subsequent mineralization of the matrix.
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골막기원세포의 조골세포로의 분화과정에서 염증성 사이토카인의 효과
박봉욱(Bong-Wook Park),최문정(Mun-Jeoung Choi),하영술(Young-Sool Hah),조희영(Hee-Young Cho),김덕룡(Deok Ryong Kim),김욱규(Uk-Kyu Kim),강희제(Hee-Jea Kang),김종렬(Jong-Ryoul Kim),변준호(June-Ho Byun) 대한구강악안면외과학회 2010 대한구강악안면외과학회지 Vol.36 No.5
Introduction: Skeletal homeostasis is normally maintained by the stability between bone formation by osteoblasts and bone resorption by osteoclasts. However, the correlation between the inflammatory reaction and osteoblastic differentiation of cultured osteoprogenitor cells has not been fully investigated. This study examined the effects of inflammatory cytokines on the osteoblastic differentiation of cultured human periosteal-derived cells. Materials and Methods: Periosteal-derived cells were obtained from the mandibular periosteum and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured in an osteogenic induction Dulbecco’s modified Eagle’s medium (DMEM) medium containing dexamethasone, ascorbic acid, and β-glycerophosphate. In this culture medium, tumor necrosis factor (TNF)-αwith different concentrations (0.1, 1, and 10 ng/mL) or interleukin (IL)-1βwith different concentrations (0.01, 0.1, and 1 ng/mL) were added. Results: Both TNF-αand IL-1βstimulated alkaline phosphatase (ALP) expression in the periosteal-derived cells. TNF-αand IL-1βincreased the level of ALP expression in a dose-dependent manner. Both TNF-αand IL-1βalso increased the level of alizarin red S staining in a dose-dependent manner during osteoblastic differentiation of cultured human periosteal-derived cells. Conclusion: These results suggest that inflammatory cytokines TNF-αand IL-1βcan stimulate the osteoblastic activity of cultured human periosteal-derived cells.
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