산성화가 식물플랑크톤 종조성에 미치는 영향

채기숙,김범철 江原大學校 附設 環境硏究所 1997 環境硏究 Vol.14 No.-

The effects of acidification on phytoplankton composition were observed by enclosure experiments. The surface water of Lake Soyang was acidified to 5.5, 5.0, or 4.5 with H₂SO₄, and the change of species composition and biomass of phytoplankton were observed. Axenic cultures of two algae, a green alga(Scenedesmus obliquus) and a cyano bacterium(Microcystis aeruginosa) was also used to test the effects of acidification. Dominant phytoplankton in Lake Soyang were Asterionella in winter, and peridinium and Anabaena in summer. In acidified condition, dominant species changed from cyanobacteria to green algae. At pH 4.5, most algal cells showed morphological damage. Species richness decreased with decreasing pH. The growth rate of Anabaena declined at below pH5.5. Whereas, the growth rate of Peridinium, Fragilaria, and Asterionella decreased only at pH 4.5. Green alga, Desmidium and Dictyosphaerium, were Anabaena, green algal increased even at pH 4.5 and grew slower at neutral pH. In axenic cultures, the growth of Microcystis aeruginosa was inhibited at 4.0 and 5.0 and showed maximum at pH 9.0. Scenedesmus obliquus also grew well at pH 5.0 and showed maximum at 9.0 At pH 4.0, Scenedesmus did not grow at all.

신성화가 식물플랑크톤 종조성에 미치는 영향

채기숙,김범철 ( Ki Sook Chae,Bomchul Kim ) 한국하천호수학회 1997 생태와 환경 Vol.30 No.3

The effects of acidification on phytoplankton composition were observed by enclosure experiments. The surface water of Lake Soyang was acidified to 5.5, 5.0, or 4.5 with H_2SO_4, and the change of species composition and biomass of phytoplankton were observed. Axenic cultures of two algae, a green alga (Scenedesmus obliquus) and a cyanobacterium (Microcystis aeruginosa) was also used to test the effects of acidification. Dominant phytoplankton in Lake Soyang were Asterionella in winter, and Peridinium and Anabaena in summer. In acidified conditions, dominant species changed from cyanobacteria to green algae. At pH 4.5 most algal cells showed morphological damage. Species richness decreased with decreasing pH. The growth rate of Anabaena declined at below pH 5.5. Whereas, the growth rate of Peridinium, Fragilaria, and Asterionella decreased only at pH 4.5. Green algae, Desmidium and Dictyosphaerium, were not dominant initially, but increased rapidly in acidified conditions. In contrast with Anabaena, green algae increased even at pH 4.5 and grew slower at neutral pH. In axenic cultures, the growth of Microcystis aeruginosa was inhibited at pH 4.0 and 5.0 and showed maximum at pH 9.0. Scenedesmus obliquus also grew well at pH 5.0 and showed maximum at pH 9.0. At pH 4.0, Scenedesmus did not grow at all.

3개의 배양액내에서 생쥐배아의 발달과 Pronase로 처리한 생쥐배아 부화율의 비교 연구

이정헌,고희정,채규정,이기숙,김종덕,Lee, Jeong-Heon,Go, Hee-Jeong,Chae, Geu-Jeong,Lee, Ki-Suk,Kim, Jong-Duk 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.3

Objectives: The purpose of this present study was to compare mouse embryo development in 3 commercial media and hatching competence of mouse embryo with or without enzymatic treatment. Methods: Collected 375 mouse embryos were divided into three groups, and then cultured in IVF-20 (G2), Medicult IVF (M3), P-1 (blastocyst M), respectively. Three day mouse morulae were cultured in G2 media treated with pronase. The results were analyzed using Chi-square test, and considered statistically significant when p<0.01. Results: The developmental rate of 2 cell mouse embryo after 72 hours was highest in IVF-20 (G2) among conventional 3 media. The hatching rate of mouse morulae was low when clultured in G2 media without pronase during 48 hours. However, it was higher when cultured in media treated with $1{\mu}g/ml$, $2.5{\mu}g/ml$, $5{\mu}g/ml$ pronase, respectively. Conclusions: Using good media and digestion of zona pellucida with enzymatic treatment improve development and hatching rate of embryo. Therefore, implantation and pregnancy rate could be improved.

국내 주요호수의 육수학적 조사 (1) : 옥정호

김범철,박주현,이병진,허우명,황길순,최광순,채기숙 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-

The limnological survey of Lake Okjong was conducted for one year from June 1993 to May 1994 on the monthly basis. The loading of phosphorus, nitrogen and organic carbon from the watershed into the lake were monitored at the main in flowing sites. Secchi disc transparency , epilimnetic chlorophyll a, total nitrogen, total phosphorus concentration and primary production were in the range of 1.3~4.H m, 2.4~ 18.7 mg Chl/m³. 1.25~2.87 mg N/l, 7~65 mg P/m³, 325~2,113 mg C/m²/day, respectively. TN/Tl atomic ratio varled from 129 to 443. N/P ratio decreased in summer because phosphorus concentration was higher than in winter, while nitrogen did not vary much. The seasonal succession of phytoplankton was distinct. In winter and spring, diatoms, Aulacoseira granulata var. angustissima and Aulacoseira italica were dominant while cyanobacteria, Microcystis sp.,M. ichthyovlabe, Phormidium sp. and P.valderianum var. tenuis were dominant in warm seasons. The dominant zooplankton species were Thertmocyclops taihokuensis in warm seasons while Boosmina longirostris were dominant in cold seasons. The organic carbon, nirtogen and phosphorus content of lake sediment were 8.0~14.8, 0.59~0.71, 1.14~1.87 ng N/g, respectively. The sediment of Lake Okjong can be classified as oligohumic based on C/N ratio. The total phosphorus loading from the watershed and fishfarm were estimated to be 2.7g P/m²/yr, which far exceeded the critical loading for eutrophication. The organic carbon loading from the watershed and primary production were determined to be 998t C/yr, 6,348t C/yr, respectively. Most of organic carbon was contributed by autochthonous primary production of phytoplankton. Trophic state of Lake Okjong can be classified as eutrophic

국내 주요호수의 육수학적 조사(3) : 진양호

김범철,박주현,허우명,임병진,황길순,최광순,채기숙 한국수생태학회(구 한국하천호수학회) 1999 생태와 환경 Vol.32 No.2

국내 주요호수의 육수학적 조사 (1) : 옥정호

김범철,박주현,임병진,허우명,황길순,최광순,채기숙 ( Bomchul Kim,Ju Hyun Park,Byung Jin Lim,Woo Myung Heo ) 한국하천호수학회 1997 생태와 환경 Vol.30 No.4

The limnological survey of Lake Okjong was conducted for one year from June 1993 to May 1994 on the monthly basis. The loading of phosphorus, nitrogen and organic carbon from the watershed into the lake were monitored at the main inflowing sites. Secchi disc transparency, epilimnetic chlorophyll a, total nitrogen, total phosphorus concentration and primary production were in the range of 1.3∼4.0 m, 2.4∼18.7 mg Chl/m^3, 1.25∼2.87 mgN/l, 7∼65 mg P/m^3, 325∼2,113 mg C/m^2/day, respectively. TN/TP atomic ratio varied from 129 to 443. N/P ratio decreased in summer because phosphorus concentration was higher than in winter, while nitrogen did not vary much. The seasonal succession of phytoplankton was distinct. In winter and spring, diatoms, Aulacoseira granulata var. angustissima and Aulacoseira italica were dominant while cyanobacteria, Microcystis sp., M.ichthyoblabe, Phormidium sp. and P. valderianum var. tenuis were dominant in warm seasons. The dominant zooplankton species were Thermocyclops taihokuensis in warm seasons while Bosmina longirostris were dominant in cold seasons. The organic carbon, nitrogen and phosphorus content of lake sediment were 8.0∼14.8, 0.59∼0.71, 1.14∼1.87mg N/g, respectively. The sediment of Lake Okjong can be classified as oligohumic based on C/N ratio. The total phosphorus loading from the watershed and fishfarm were estimated to be 2.7 g P/m^2/yr, which far exceeded the critical loading for eutrophication. The organic carbon loading from the watershed and primary production were determined to be 998t C/yr, 6,348t C/yr, respectively. Most of organic carbon was contributed by autochthonous primary production of phytoplankton. Trophic state of Lake Okjong can be classified as eutrophic.

국내 주요호수의 육수학적 조사 3 : 진양호

김범철(Kim Bomchul),박주현(Ju-Hyun Park),허우명(Woo-Myung Heo),임병진(Byung-Jin Lim),황길순(Gilson Hwang),최광순(Kwangsoon Choi),채기숙(Ki-Sook Chae) 강원대학교 환경연구소 1999 環境硏究 Vol.16 No.-