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RISS 인기검색어
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- 저자 : 진휘승(Hwi Seung Chin) (검색결과 4 건)
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진휘승(Hwi Seung Chin),손락호(Rak Ho Son),이용화(Yong-Hwa Lee),함아름(Ahrom Ham),마응천(Woongchon Mar),김원기(Won-Ki Kim),남궁우(Kung-Woo Nam) 대한약학회 2010 약학회지 Vol.54 No.2
The chemical structures of eight compounds purified from two plants (Polygala tenuifolia Willdenow and Dioscorea batatas Decene) were determined and their anti-microbial activity against three microbial strains (Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans) was tested. The three micro organisms were cultured in 96-well plates or Petri dishes without (control) or with the eight compounds added at concentrations of 100 to 0.01 μM (wt/vol). The growth of the microorganisms in the medium was examined after a 24-h incubation. The inhibitory effect of each compound on the growth of the microorganisms was calculated from the optical density measured at 595 nm, turbidity, and size of the inhibition zone around the treated paper disc. The minimum inhiitory concentration (MIC) of compounds 4 to 7 against S. aureus was 0.08, 0.05, 1.3 and 0.02 μM, respectively, and 0.09, 0.1, 0.2 and 100 μM against C. albicans. The IC50 (50% inhibition) values of compounds 5 and 6 were 3.1 and 6.4 μM against S. aureus, respectively, and 10 and 2.4 μM against C. albicans. Therefore, compounds 4 to 6 were the most potent anti-microbial agents among the eight compounds tested.
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진휘승(Hwi Seung Chin),박금주(Keum Ju Pack),박승희(Seung Hee Pack),김재기(Jae Ki Kim) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.1
민간요법 중에서 비만 치료에 사용되는 4종의 식물인 지유, 옥발, 적하수오 및 삼백초를 동량으로 혼합하여 10배의 물을 사용하여 가열추출 후 감압농축 하여 HEM을 조제하였고, 이렇게 조제된 HEM을 사용하여 0~100 ㎍/mL 농도로 3T3-L1의 지방축적 억제율을 측정한 결과 농도가 증가함에 따라 유의적으로 증가하였으며, 동일 농도에서 세포독성은 없었다. 또한 지방 축적에 관여하는 효소인 GPDH의 활성을 억제함으로써 세포내 지방이 축적되는 것을 억제하였다. 5주간의 동물실험 결과 대조군과 비교하여 HEM1과 HEM2군 모두 체중 변화와 식이효율이 줄어드는 것으로 나타났으며, 혈액 분석에서 HEM군은 혈중 중성지방과 총콜레스테롤에 효과를 보이지 않았다. 해부학적인 측면에서 부고환 및 신장 주위의 지방무게와 간 무게 또한 낮았다. 이러한 결과를 통해, 본 실험에서 사용한 생약복합물은 항비만 소재로써 체지방 축적억제에 효과성이 있음을 확인하였다. In this study, we investigated the anti-obesity activity of the herbal extract mixture (HEM). The inhibitory effect of HEM on triglyceride accumulation of 3T3-L1 preadipocyte was examined by Oil-Red O staining. HEM inhibited the triglyceride accumulation of 3T3-L1 preadipocyte cell and reduced glycerol-3-phosphate dehydrogenase (GPDH) activity. We further investigated the effect of HEM in prevention of obesity in male ICR mouse for 5 weeks. Experimental groups were divided into high fat diet group (HFD), HFD supplemented with 100 ㎎/㎏ HEM group (HEM1) and HFD supplemented with 200 ㎎/㎏ HEM group (HEM2). Body weight and food efficiency ration of HEM1 and HEM2 was decreased by 52% and 50% and by 45% and 50%, respectively. The amount of adipocyte in body weight was decreased. Blood triglyceride and total cholesterol of HEM1 was significantly decreased. These results indicate that HEM may be useful in preventing obesity.
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뽕나무에서 분리한 Steppogenin과 Oxyresveratrol의 효모 α-Glucosidase의 억제효과
진휘승(Hwi Seung Chin),남궁우(Kung-Woo Nam) 대한약학회 2010 약학회지 Vol.54 No.5
α-Glucosidase inhibitor is a target in the treatment of type II diabetes through the mainly inhibition of glucose levels after meals. In this study, we purified steppogenin and oxyresveratrol from the stem of Morus alba L. and examined their inhibitory activity against yeast α-glucosidase. Steppogenin and oxyresveratrol were inhibited yeast α-glucosidase in a dose dependent manner. The IC50 activities (50% inhibition) were 34.4 and 9.3 μM, respectively. The kinetic inhibition of steppogenin showed noncompetitive inhibition (Km: 1.1×10-3 M; Ki: 1×10-5 M), meanwhile oxyresveratrol showed competitive inhibition (Km: 4.3×10-3 M; Ki: 3.4×10-6 M) against yeast α-glucosidase. These results indicate that steppogenin and oxyresveratrol are noncompetitive and competitive inhibitors, respectively, against yeast α-glucosidase.
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머루 줄기와 자소자로부터 분리한 Resveratrol 올리고머와 Flavonoid의 항균효과
손락호(Rak Ho Son),진휘승(Hwi Seung Chin),함아름(Ahrom Ham),마응천(Woongchon Mar),남궁우(Kung-Woo Nam) 대한약학회 2010 약학회지 Vol.54 No.1
We studied the antimicrobial activities of five compounds isolated from the stems of Vitis coignetiae Pulliat and the seeds of Perilla frutescens (L.) Britton. Based on spectroscopic evidence, compounds 1 to 5 were characterized as resveratrol, ε-viniferin, ampelopsin E, apigenin, and luteolin, respectively. The antimicrobial activities against Gram-positive (Staphylococcus aureus) and -negative (Pseudomonas aeruginosa) bacteria and a fungus (Candida albicans) were investigated using the disc diffusion and broth dilution methods. C. albicans was not inhibited by the five compounds. Compounds 2 and 5 had significant anti-microbial activity against S. aureus, and the 50% inhibitory concentration (IC50) of compound 2 against S. aureus was 7.2 μM. Compounds 4 and 5 significantly inhibited P. aeruginosa and the minimum inhibitory concentration (MIC) of compounds 2 and 5 was 0.07 and 2.0 μM, respectively. Compounds 2, 4, and 5 had strong anti-microbial activity against S. aureus and P. aeruginosa.
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