저산소-허혈 손상이 신생 흰쥐의 뇌 선조체(Striatum) Monoamine 대사에 미치는 영향

지윤희,김형건,박우성,장영표,Jee, Youn Hee,Kim, Hyung Gun,Park, Woo Sung,Chang, Young Pyo 대한소아청소년과학회 2003 Clinical and Experimental Pediatrics (CEP) Vol.46 No.8

목 적: 신생 흰쥐의 뇌에 저산소-허혈을 유발하여 선조체 monoamine과 그 대사물들의 변화를 관찰하여, 저산소-허혈 손상 시 dopamine과 monoamine의 역할을 관찰하고자 하였다. 방 법 : 생후 5-6일 된 신생 흰쥐의 우측 총경동맥을 결찰 후 우측 선조체에 microdialysis probe를 삽입하였다. 2시간 동안의 안정기를 거친 후, probe를 통해 기저치를 수집하고, 바로 8% 산소에 2시간 동안 노출시키고, 2시간 동안 회복시키며 20분 간격으로 수집한 샘플을 HPLC를 통해 분석하였다. 결 과 : 1) Dopamine은 저산소-허혈기에 급격히 증가하는 경향을 보였으나 통계적 유의성은 없었다(P>0.05). 2) DOPAC은 저산소-허혈 동안 기저치의 $23.0{\pm}4.2%$까지 감소하였다가, 재산소화 동안에 기저치의 $120.8{\pm}54.9%$까지 증가하였다(P<0.05). 3) HVA는 DOPAC과 같은 변화를 보였으나 덜 현저하였고, 저산소-허혈 동안 기저치의 $35.3{\pm}7.6%$까지 감소하였다가 재산소화 동안에 $105.8{\pm}32.3%$까지 회복되었다(P<0.05). 4) NE은 저산소-허혈 노출과 재산소화 동안 유의한 변화를 보이지 않았다(P>0.05). 5) 5-HIAA는 저산소-허혈 동안 서서히 감소하였다가 재산소화 동안 증가하였고, 그 변화는 통계적으로 유의하였다(P<0.05). 6) 실험 중 serotonin은 검출되지 않았다. 결 론: 저산소-허혈은 미성숙 신생 횐쥐의 뇌 선조체의 monoamine 대사에 영향을 끼쳤으며, 이 결과는 monoamine, 특히 dopamine과 그 대사물들이 신생 흰쥐 뇌의 저산소-허혈손상의 기전에 중요한 역할을 할 수 있다는 가능성을 제시한다. Purpose : We intended to evaluate the effect of hypoxia-ischemia on extracellular striatal monoamine metabolism in neonatal rat brains by in vivo microdialysis. Methods : The right common carotid arteries of five or six-day old rats were surgically ligated, and the probes for microdialysis were inserted into the right striatum with stereotaxic instrument. After stabilization for two hours, artificial cerebrospinal fluid was infused via the probe for microdialysis and samples were collected during hypoxia-ischemia and recovery periods at 20 minute intervals. The concentrations of DA(dopamine), DOPAC(3,4-di-hydroxyphenyl acetic acid), HVA(homovanillic acid), NE(norepinephrine), and 5-HIAA(5-hydroxy indole-acetic acid) were measured by HPLC(high performance liquid chromatography) and the changes were analysed. Results : The striatal levels of dopamine metabolites such as DOPAC and HVA, were significantly decreased during hypoxia-ischemia, and increased to their basal level during reoxygenation(P<0.05). Dopamine mostly increased during hypoxia but statistically not significant(P>0.05). DOPAC showed the most remarkable decrease($23.0{\pm}4.2%$, P<0.05), during hypoxia-ischemia and increase to the basal levels during reoxygenation($120.8{\pm}54.9%$, P<0.05), and HVA showed the same pattern of changes as those of DOPAC during hypoxia-ischemia($35.3{\pm}7.6%$ of basal level, P<0.05) and reoxygenation ($105.8{\pm}32.3%$). However, the level of NE did not show significant changes during hypoxia-ischemia and reoxygenation. The levels of 5-HIAA decreased($74.9{\pm}3.1%$) and increased($118.1{\pm}7.8%$) during hypoxia-ischemia and reoxygenation, respectively(P<0.005). Conclusion : Hypoxia-ischemia had a significant influence on the metabolism of striatal monoamine in neonatal rat brains. These findings suggest that monoamine, especially dopamine, and its metabolites could have a significant role in the pathogenesis of hypoxic-ischemic injury of neonatal rat brains.

항적 산란신호의 모델링과 실험적 검증

지윤희,이재훈,김재수,김정해,김우식,최상문,Ji, Yoon-Hee,Lee, Jae-Hoon,Kim, Jea-Soo,Kim, Jung-Hae,Kim, Woo-Shik,Choi, Sang-Moon 한국음향학회 2009 韓國音響學會誌 Vol.28 No.1

이동하는 수상 운동체는 반경 $8{\sim}200{\mu}m$ 크기의 미세기포군을 포함하는 기포항적을 발생시킨다. 경우에 따라 10여분 이상 지속되는 수중 미세기포는 음향산란을 일으키는 요인이 되며, 기포가 존재하는 동안 능동소나에 의한 지속적인 탐지가 가능하다. 본 논문에서는 기포항적에 존재하는 미세기포의 사공간적인 분포변화에 따라 산란되는 음파를 모의하는 잔향음 모델을 제시하였다. 기포항적 산란신호의 모델은 음향학적 모델과 운동학적 모델로 이루어져 있으며 음향학적 모델에서는 미세기포의 공간분포를 체적산란강도로 변환하여 공간에 대한 적분을 수행하고, 운동학적 모델은 해양공간의 고정좌표계와 능동소나에 고정된 국부좌표계 사이의 좌표변환을 위한 오일러변환을 기반으로 구현되었다. 구현된 모델의 점증을 위해 2007년 9월 한국해양대학교 앞 해상에서 실제 선박을 운항하여 기포항적을 발생시킨 후 일정간격으로 신호를 획득하여 분석하였고, 이를 모델에 적용하여 타당성을 검증하였다. A moving surface vessel generates a ship wake which contains a cloud of micro-bubbles with radii ranging between $8{\sim}200{\mu}m$. Such micro-bubbles can be detected by active sonar system for more than ten minutes depending on the size and speed of the surface vessel. In this paper, a reverberation model for the ship wake is presented. The developed model consists of the acoustic scattering model due to the distribution of the micro-bubbles and the kinematic model for the moving active sonar. The acoustic scattering model is based on the volume integration, where the volume scattering strengths are obtained from the spatial distribution of micro-bubbles. Since the directivity and look-direction of active sonar are important factors for moving active sonar, the kinematic model utilizes the Euler transformation to obtain the relative motion between the global and local coordinates. In order to verify the developed model, a series of sea experiment was executed in September 2007 to obtain the spatial-temporal distribution of a bubble cloud, and analyzed to be compared with the simulation results.

빛과 색채의 조화

지윤희 중앙대학교 예술대학 회화학과 1976 卒業作品硏究報告書集 - 中央大學校 藝術大學 繪畵學科 Vol.1976 No.-

임상연구 특성요소가 IRB의 임상연구관리에 미치는 영향

지윤희,방준석,김영인,김경수,나현오 대한임상약리학회 2008 Translational and Clinical Pharmacology Vol.16 No.2

Background: The number of clinical trials which have been performed within Korea is increasing accompanied by the enlargement of the various responsibilities of each Institutional Review Board (IRB) in the way of providing adequate and on-time managerial involvements on the studies. Many confounding factors of each study can influence on the results with which delaying the final reports, or extending the review times associated with research-periods, or adjusting the alterations. Even though these factors were closely related to the results being managed by the IRBs, no in-depth research have been implemented to elucidate the correlations between these factors and the results necessitates IRBs’ managements. Methods: Study subjects were selected and analyzed retrospectively in a University hospital’s database. About 190 study projects which had been completed during 2005 to 2007 were retrieved and the characterized factors were classified into seven categories. Each category was analyzed and tested statistically by SAS program. Results: It is revealed that a large portion of the factors was subjected to the study profiles. The review-time showed extended tendency in the subjects which used medications (P=0.0174) required approval (P=0.0009) in phases ⅠⅡⅢ (P<0.0001) initiated by sponsors (P<0.0001) and with duration of 1 to 2 years (P=0.0442). Prior to submitting IRB, correction-times were multiplied specifically on the study subjects that used medications (P<0.0001) required approvals (P<0.0001), underwent phase ⅠⅡⅢ (P<0.0001) reviewed twice (P=0.0074) initiated by sponsors (P<0.0001) exceeded 2 years of period (P<0.0001) and required modifications before the submission (P=0.0001). Conclusion: Theclinical study projects characterize by ‘medication-used’, ‘sponsor-initiated’, ‘approval-required’, ‘duration period of 1 to 2 years’, and ‘under phase ⅠⅡ Ⅲ’ required thorough concerns and investigations from the beginning stage to reduce the time of review by the IRBs. Studies lasted more than 2 years entailed preparation of the sudden alterations to prevent the study duration. A new further study is necessary to analyze the frequency and the causes of the alterations in the study proposals. Background: The number of clinical trials which have been performed within Korea is increasing accompanied by the enlargement of the various responsibilities of each Institutional Review Board (IRB) in the way of providing adequate and on-time managerial involvements on the studies. Many confounding factors of each study can influence on the results with which delaying the final reports, or extending the review times associated with research-periods, or adjusting the alterations. Even though these factors were closely related to the results being managed by the IRBs, no in-depth research have been implemented to elucidate the correlations between these factors and the results necessitates IRBs’ managements. Methods: Study subjects were selected and analyzed retrospectively in a University hospital’s database. About 190 study projects which had been completed during 2005 to 2007 were retrieved and the characterized factors were classified into seven categories. Each category was analyzed and tested statistically by SAS program. Results: It is revealed that a large portion of the factors was subjected to the study profiles. The review-time showed extended tendency in the subjects which used medications (P=0.0174) required approval (P=0.0009) in phases ⅠⅡⅢ (P<0.0001) initiated by sponsors (P<0.0001) and with duration of 1 to 2 years (P=0.0442). Prior to submitting IRB, correction-times were multiplied specifically on the study subjects that used medications (P<0.0001) required approvals (P<0.0001), underwent phase ⅠⅡⅢ (P<0.0001) reviewed twice (P=0.0074) initiated by sponsors (P<0.0001) exceeded 2 years of period (P<0.0001) and required modifications before the submission (P=0.0001). Conclusion: Theclinical study projects characterize by ‘medication-used’, ‘sponsor-initiated’, ‘approval-required’, ‘duration period of 1 to 2 years’, and ‘under phase ⅠⅡ Ⅲ’ required thorough concerns and investigations from the beginning stage to reduce the time of review by the IRBs. Studies lasted more than 2 years entailed preparation of the sudden alterations to prevent the study duration. A new further study is necessary to analyze the frequency and the causes of the alterations in the study proposals.

최근의 한국영화에 나타난 '서울' 읽기 : <홀리데이 인 서울> <초록 물고기> <돼지가 우물에 빠진 날>을 중심으로

지윤희 한국예술종합학교 영상원 영상이론과 1999 영화문화연구 Vol.- No.1

WHICH WEIGHTED SHIFTS ARE M-HYPONORMAL?

지윤희 충청수학회 2022 충청수학회지 Vol.35 No.1

Let $\alpha=\{\alpha_n\}_{n=0}^{\infty}$ be a weight sequence and let $W_\alpha$ denote the associated unilateral weighted shift on $l^2$($Z_+$). In this paper we will investigate which weighted shift is M-hyponormal.

Ladle Turret 용 내압 300 bar 급 Rotary Joint 구조해석

지윤희(Y. H. Ji),구현주(H. J. Koo) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.5월

다중상태 소나시스템을 적용한 표적반향음 연구

지윤희(Yoon Hee Ji),배호석(Ho Seuk Bae),변기훈(Gi-Hoon Byun),김재수(Jea Soo Kim),김우식(Woo-Shik Kim),박상윤(Sang-Yoon Park) 한국해양공학회 2014 韓國海洋工學會誌 Vol.28 No.5

A multi-static SONAR system consists of the transmitters and receivers separately in space. The active target echoes are received along the transmitter-target-receiver path and depend on the shape and aspect angle of the submerged objects at each receiver. Thus, the target echo algorithm used with a mono-static system, in which the transmitter and receiver are located at the same position, has limits in simulating the target echoes for a multi-static SONAR system. In this paper, a target echo modeling procedure for a 3D submerged object in space is described based on the Kirchhoff approximation, and the SONAR system is extended to a multi-static SONAR system. The scattered field from external structures is calculated on the visible surfaces, which is determined based on the locations of the transmitter and receiver. A series of experiments in an acoustic water tank was conducted to measure the target echoes from scaled targets with a single transmitter and 16 receivers. Finally, the numerical results were compared with experimental results and shown to be useful for simulating the target echoes/target strength in a multi-static SONAR system.

300 bar 의 압력을 받는 Swivel Joint 의 구조 안정성 해석

지윤희(Y. H. Ji),구현주(H. J. Koo) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.5월

신생아에서 제대 동맥혈 Isoprostane(8-iso-PGF₂_α) 농도에 관한 연구

이건송,지윤희,장영표,Lee, Kun Song,Ji, Yoon Hee,Chang, Young Pyo 대한소아청소년과학회 2003 Clinical and Experimental Pediatrics (CEP) Vol.46 No.9

목 적 : 지질 과산화의 주요 지표 중에 하나인 isoprostane($8-iso-PGF_{2{\alpha}}$)을 제대 동맥혈에서 측정하여 신생아에 산화손상을 유발 할 수 있는 주산기 위험인자 및 신생아기 주요 질환과의 관계를 규명하여, 제대 동맥혈 isoprostane이 신생아에서 산화손상의 지표로 사용할 수 있는지를 알아보고자 하였다. 방 법 : 2000년 6월부터 2001년 3월까지 단국대학교병원 신생아 중환자실 및 신생아실에 입원하였던 미숙아 33명과 만삭아 28명을 대상으로 제대 동맥혈에서 혈청을 분리하여 $-70^{\circ}C$에서 냉동 보관 후 isoprostane($8-iso-PGF_{2{\alpha}}$)과 malondialdehyde(MDA)를 측정하였다. 측정된 isoprostane과 MDA 농도를 미숙아와 만삭아에서 각각 비교하였고, 주산기-신생아기 위험인자와 주요 합병증과의 상관관계를 알아보았다. 결 과 : 평균 출생 체중은 미숙아 $1,771{\pm}445gm$, 만삭아 $3,200{\pm}567gm$이었고, 평균 재태 연령은 미숙아 $31.5{\pm}2.0$주, 만삭아 $39.0{\pm}2.0$주였다. 제대 동맥혈 isoprostane 농도는 미숙아 $704.7{\pm}635.6pg/mL$, 만삭아 $423.9{\pm}306.5pg/mL$로 미숙아에서 통계적으로 의미있게 높았으며(P<0.05), MDA도 미숙아 $44.0{\pm}22.9{\mu}M/L$, 만삭아 $28.2{\pm}10.7{\mu}M/L$로 미숙아에서 의미있게 높았다(P<0.05). 미숙아의 경우 isoprostane은 출생 후 24시간에 $478.6{\pm}580.6pg/mL$로 유의하게 감소하였다(P<0.05). 미숙아 제대 동맥혈 isoprostane은 둔위 분만, 양수 과소증, 신생아 가사와 통계적으로 유의한 상관관계가 있었고(P<0.05), 만삭아 제대 동맥혈 isoprostane은 임신성 고혈압과 유의한 상관관계를 보였다(P<0.05). 그러나 미숙아 제대 동맥혈 isoprostane은 신생아기의 주요 합병증과는 상관관계가 없었다. 결 론 : 미숙아에서 제대 동맥혈 isoprostane 농도는 만삭아에 비하여 높고, 일부 주산기-신생아기 위험인자와 연관이 있어서, 주산기-신생아기에 산화손상과 관련된 주요 지표 중에 하나로 사용될 가능성이 있음을 추측하였다. Purpose : We measured the umbilical cord arterial concentrations of isoprostane($8-iso-PGF_{2{\alpha}}$) and intended to decide whether the umbilical cord arterial concentrations of isoprostane could be used as a useful parameter for lipid peroxidation in newborn infants. Methods : The isoprostane and malondialdehyde(MDA) concentrations of the umbilical cord were measured by enzyme immunoassay and TBARS(thiobarbituric acid reactive substance) assay in 33 preterm and 28 term infants, respectively. The concentrations of isoprostane and MDA were compared between preterm infants and term infants, and were analysed for association with perinatal risk factors and neonatal complications. Results : Umbilical cord arterial concentrations of isoprostane were $704.7{\pm}635.6pg/mL$ and $421.9{\pm}306.5pg/mL$ in preterm and term infants, respectively. Umbilical cord arterial concentrations of MDA were $44.0{\pm}22.9{\mu}M/L$ and $26.2{\pm}10.7{\mu}M/L$ in preterm and term infants, respectively. Umbilical cord arterial concentrations of isoprostane and MDA in preterm infants were significantly higher than those in term infants(P<0.05). The umbilical cord arterial concentrations of isoprostane were significantly associated with perinatal risk factors such as fetal distress, oligohydramnios, and breech delivery in preterm infants and pregnancy-induced hypertension in term infants(P<0.05). Conclusion : Umbilical cord arterial concentrations of isoprostane in preterm infants were higher than those in term infants, and those are significantly associated with some perinatal risk factors.