http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 주충노 ( Young Don Lee (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
쥐 뇌에서의 Aldehyde Dehydrogenase의 분포에 관한 연구
이영돈,주충노,Lee, Young-Don,Joo, Chung-No 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.8
쥐 뇌에서의 aldehyde dehydrogenase(ALDH) 활성분포를 조사하기 위하여 합성된 생체 aldehyde를 비롯한 여러 기질을 사용하여 부위에 따른 활성, 세포내 분포 및 isozyme의 존재 양상을 조사하였다. 뇌의 모든 부위에서 indole-3-acetaldehyde에 대한 총 ALDH활성은 acetaldehyde나 propionaldehyde에 대한 활성보다 높았다. $V_{max}/K_{m}$비를 통하여 기질과의 친화도를 조사한 결과 indole-3-acetaldehyde와 propionaldehyde에 대한 친화도는 뇌의 모든 부위에서 low-$K_m$ ALDH가 $high-K_m$, ALDH보다 훨씬 컸다. 특히 선조체의 ALDH는 다른 부위의 ALDH에 비하여 방향족 aldehyde에 대하여 낮은 $K_m$ 값과 높은 $V_{max}/K_{m}$비를 나타냈으며 3,4-dihydroxyphe-nylacetaldehyde(DOPAL)에 대하여 높은 활성을 나타내었다. 이같은 결과는 뇌조직에서 신경전달물질로부터 유래한 방향족 aldehyde는 주로 $low-K_m$ ALDH에 의해 산화되며 이 과정은 특히 선조체에서 dopamine의 산화과정에 크게 관여할 것으로 생각된다. Indole-3-acetaldehyde에 대한 ALDH활성의 세포내 분포의 경우, 미토콘트리아분획의 활성은 시상하부와 해마체에서 다소 높았고 미크로좀분획의 활성은 뇌의 모든 부위에서 가장 낮은 분포를 보였으며 부위에 따라 뚜렷한 차이가 없었다. 뇌 ALDH는 isoelectric focusing에 의해 두 분획(E1, E2)으로 구성된 $low-K_m$ isozyme과 활성이 미약한 $high-K_m$ isozyme으로 분리되었으나 부위에 따른 분포양상의 차이는 관찰되지 않았다. This study was attempted to examine the ALDH activities using several aldehydes including synthetic biogenic aldehydes and the subcellular distribution in seven different areas of rat brain. The total ALDH activity with indole-3-acetaldehyde were higher than acetaldehyde and propionaldehyde in all areas. Taking $V_{max}/K_{m}$ ratio as the index of affinity of substrate to the enzyme, affinities of $low-K_m$, ALDH for indole-3-acetaldehyde and propionaldehyde were stronger than those of $high-K_m$, ALDH. Especially striatum showed the lowest $K_m$ value and the highest $V_{max}/K_{m}$ ratio for aromatic aldehyde and contained the highest activity with biogenic aldehyde such as DOPAL. From these results, it is suggested that brain low $K_m$ ALDH may play an important role in degradation of neurotransmitter-derived aromatic aldehydes, especially dopamine oxidation in the striatum. ALDH activity of mitochondrial fraction was slighltly high in the hypothalamus and hippocampus. Microsomal ALDH activity was lowest in all brain areas and a significant regional difference was not found. Although two $low-K_m$, isozymes, composed of cytosolic E1 and mitochondrial E2, and very weak high $K_m$ isozymes were identified by isoelectric focusing, their distribution pattern in various areas was not different.
-
쥐 뇌에서의 Aldehyde Dehydrogenase 의 분포에 관한 연구
이영돈,주충노 ( Young Don Lee,Chung No Joo ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.8
This study was attempted to examine the ALDH activities using several aldehydes including synthetic biogenic aldehydes and the subcellular distribution in seven different areas of rat brain. The total ALDH activity with indole-3-acetaldehyde were higher than acetaldehyde and propionaldehyde in all areas. Taking V_(max)/K_m ratio as the index of affinity of substrate to the enzyme, affinities of low-Km ALDH for indole-3-acetaldehyde and propionaldehyde were stronger than those of high-Km ALDH. Especially striatum showed the lowest K_m value and the highest V_(max)/K_m raatio for aromatic aldehyde and contained the highest activity with biogenic aldehyde such as DOPAL. From these results, it is suggested that brain low K_m ALDH may play an important role in degradation of neurotransmitter-derived aromatic aldehydes, especially dopamine oxidation in the striatum. ALDH activity of mitochondrial fraction was slighltly high in the hypothalamus and hippocampus. Microsomal ALDH activity was lowest in all brain areas and a significant regional difference was not found. Although two low-K_m isozymes, composed of cytosolic E1 and mitochondrial E2, and very weak high K_m isozymes were identified by isoelectric focusing, their distribution pattern in various areas was not different.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
