http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
주원아,김민영,이은예,박경숙 ( Won A Joo,Min Young Kim,Eun Yee Lee,Kyung Sook Park ) 한국유전학회 1995 Genes & Genomics Vol.17 No.2
Human aldehyde dehydrogenase (ALDH, E.C. 1. 2. 1. 3.) which oxidizes acetaldehyde to acteic acid during alcoholic metabolism, contains two major isozymes, the cytosolic ALDH1 and the mitochondrial ALDH2. The genotype of ALDH2 genes was typed in 311 normal Korean. For ALDH2 gene typing, genomic DNA was extracted from leukocyte and Exon 12 of ALDH2 gene was amplified by polymerase chain reaction. Amplified gene was hybridized with allele-specific oligonucleotide probe on nylon membrane and detected using colorimetric detection method. The frequencies of ALDH2 genotypes ALDH2*1|ALDH2*1, ALDH2*1|ALDH2*2 and ALDH2*2|ALDH2*2 were 60.8%, 34.1% and 5.1%, respectively. The estimated allele frequencies for ALDH2*1 and ALDH2*2 were 0.78 and 0.22, respectively. The allele frequencies of ALDH2 vary in different ethnic groups. The allele frequency of ALDH2*2 increased in Mongoloid; Korean was 0.22, Chinese was 0.24, and Japanese was 0.35, while the genotype of ALDH2*2|ALDH2*2 has not been observed yet in European populations.
Comparison of Search Engine Contributions in Protein Mass Fingerprinting for Protein Identification
김찬화,주원아,이재원,박미라,이정복,Hyun-Jung Kim 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.2
Peptide mass fingerprinting (PMF) is a valuable method for rapid and high-throughput protein identification using the proteomics approach. Automated search engines, such as Ms-Fit, Mascot, ProFound, and PeptIdent, have facilitated protein identification through PMF. The potential to obtain a true MS protein identification result depends on the choice of algorithm as well as experimental factors that influence the information content in MS data. When mass spectral data are incomplete and/or have low mass accuracy, the “number of matches” approach may be inadequate for a useful identification. Several studies have evaluated factors influencing the quality of mass spectrometry (MS) experiments. Missed cleavages, post-translational modifications of peptides and contaminants (e.g., keratin) are important factors that can affect the results of MS analyses by influencing the identification process as well as the quality of the MS spectra. We compared search engines frequently used to identify proteins from Homo sapiens and Halobacterium salinarum by evaluating factors, including database and mass tolerance, to develop an improved search engine for PMF. This study may provide information to help develop a more effective algorithm for protein identification in each species through PMF.