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        조직배양한 자호근의 Saikosaponin 함량

        조필형(Pil Hyung Jo),성낙선(Rack Seon Seong),배형화(Hyung Hwa Bae),소웅영(Woong Young Soh),조덕이(Duck Yee Cho) 한국생약학회 1990 생약학회지 Vol.21 No.3

        The study for the quantitative analysis of saikosaponin of Bupleurum falcatum root produced by tissue culture was attempted. The optimal concentration of 2, 4-D was 0.1 ㎎/ℓ for inducing the callus. The induction and growth of the adventitious root from the callus was obtained in the suspension culture containing MS basal medium supplemented with no plant growth regulators. The results of the quantitative analysis of saikosaponins by high performance liquid chromatography (HPLC) showed that the content of saikosaponin a and c was high in the one-year-old root from somatic embryos and that of saikosaponin d was remarkably high in three-months-old adventitious root from callus.

      • 組織培養한 柴胡根의 Saikosapnin含量

        趙弼衡,成樂宣,裵馨華,蘇雄永,趙德以 全北大學校 基礎科學硏究所 1991 基礎科學 Vol.14 No.2

        The study for the quantitative analysis of saikosaponin of Bupleurum falcatum root produced by tissue culture was attempted. The optimal concentration of 2, 4-D was 0.1㎎/l for inducing the callus. The induction and growth of the adventitious root from the callus was obtained in the suspension culture containing MS basal medium supplemented with no plant growth regulators. The results of the quantitative analysis of saikosaponins by high performance liquid chromatography (HPLC) showed that the content of saikosaponin a and c was high in the one-year-old root from somatic embryos and that of saikosaponin d was remarkably high in three-months-old adventitious root from callus.

      • 랫트의 鉛中毒에 대한 BHA의 影響에 관한 硏究

        趙弼衡 圓光大學校大學院 1981 學位論叢 Vol.6-2 No.-

        An attempt has been made to evaluate the effect cf antioxidant; butylated hydroxyanisole (BHA), on rat lead poisoning. 360 Female albino rats were divided into 36 experimental groups. All the groups were fed with diets formulated by adding BHA in a range of 0.1% to 3.2%. Aqueous solution of 1% lead acetate was supplied as drinking water to all the experimental groups except the control. Experimental animals from each group were sacrificed on every two weeks interval and twelve various organs such as salivary gland, thymus, lung, heart, liver, pancreas, spleen, kidney, ovary, brain, pituitary and lymph were removed and weighed. Lead sedimentation in kidney tissue was examined through microscopic observation. Intake of food and water was recorded on weekly basis. Serological study including GPT and GOT values were also carried out after sacrificing animals from various groups. It was concluded, after analyzing the data obtained, that the antioxidant BHA is effective for reducing the toxic effect of lead acetate on rats. The reducing effect of BHA on lead poisoning was enhanced significantly by feeding BGA for an enhanced period, namely eight weeks.

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