Soybean Storage Protein Glycinins and Their genes

조태주 한국생화학분자생물학회 1989 생화학분자생물학회 소식 Vol.9 No.4

Isolation and Characterization of Chlorophyll a/b Binding Protein Genes in Soybean

조태주,정기아,채쾌,Cho, Tae-Ju,Chung, Kee-A,Chae, Quae 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.5

빛에 의한 식물체 유전자 발현의 조절기작을 이해하기 위하여, 대두에서 빛에 의해 그 발현이 조절되는 유전자를 클로닝하는 작업을 일차적으로 실시하였다. cab은 엽록체에 존재하는 chlorophyll 결함 단백질의 유전자로, 빛의 존재하에서 또는 암소에서 키운 대두 종묘에서 추출한 mRNA를 사용하여 Northern blot hybridization을 수행한 결과, 대두의 cab 유전자 발현이 빛에 의해 대폭 증진됨이 확인되었다. 이에 대두의 cab 유전자를 클로닝하기 위해 cDNA library를 제조한 다음 screening 작업을 실시하였다. 분리 동정된 9개 의 ${\lambda}$ cab cDNA clone중 두 개 clone의 insert를 subcloning하여 각각 pGAB3와 pGAB7이라 명명하였다. 이들 두 cDNA clone들은 각각 다른 cab gene에 대한 cDNA clone인 것으로 밝혀졌으며, 3' untranslated region에서의 염기서열이 매우 다른 것으로 나타났다. pGAB3은 31 nucleotide의 5' untranslated region을 포함한 full-length cDNA clone이며, 32개 아미노산으로 구성된 transit peptide를 갖고 있는 것으로 나타났다. To investigate how light regulates plant gene experssion, we initiated a research program to isolate light-regulated soybean cab genes encoding chlorophyll a/b-binding proteins. For this purpose, a soybean cDNA library was constructed and screened. The inserts from three out of the nine soybean ${\lambda}$ cab clones obtained were subcloned into pBluescribe+, and designated pGAB3, pGAB7 and pGAB11. The restiction maps of pGAB3 and pGAB7 were found to be considerably different from each other. Further characterization of the two clones by dot blot hybridization and sequence analysis showed that the two soybean cab clones represent distinct cab genes in soybean. pGAB3, which is very homologous to the pea cab clone pAB96, has a full-length cDNA insert of 1.0 kb in size. pGAB7 has a 0.85 kb insert containing a truncated coding sequence. The 3' untranslated regions of the pGAB3 and pGAB7 are so divergent that any homologous regions can not be identified. pGAB11 was found to be very homologous to the pGAB3, and it remains to be determined whether the pGAB11 represents a distinct cab gene.

대두 cab 유전자의 cDNA 클로닝 및 발현 조사

조태주,정기아,채쾌 ( Tae Ju Cho,Kee A Chung,Quae Chae ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.5

To investigate how light regulates plant gene experssion, we initiated a research program to isolate light-regulated soybean cab genes encoding chlorophyll a/b-binding proteins. For this purpose, a soybean cDNA library was constructed and screened. The inserts from three out of the nine soybean λ cab clones obtained were subcloned into pBluescribe^+, and designated pGAB3, pGAB7 and pGAB11. The restiction maps of pGAB3 and pGAB7 were found to be considerably different from each other. Further characterization of the two clones by dot blot hybridization and sequence analysis showed that the two soybean cab clones represent distinct cab genes in soybean. pGAB3, which is very homologous to the pea cab clone pAB96, has a full-length cDNA insert of 1.0 kb in size. pGAB7 has a 0.85 kb insert containing a truncated coding sequence. The 3` untranslated regions of the pGAB3 and pGAB7 are so divergent that any homologous regions can not be identified. pGAB11 was found to be very homologous to the pGAB3, and it remains to be determined whether the pGAB11 represents a distinct cab gene.

Computer Simulation을 통한 Subtractive Hybridization 실험의 예측 및 최적화

조태주 忠北大學校 遺傳工學硏究所 1998 遺傳工學硏究 Vol.12 No.1

Signal Transduction and Regulation of Gene Expression in Phytochrome Mediated Photomorphogenesis

조태주 忠北大學校 遺傳工學硏究所 1990 遺傳工學硏究 Vol.4 No.2

API 특성 정보기반 악성 애플리케이션 식별 기법

조태주(Taejoo Cho),김현기(Hyunki Kim),이정환(Junghwan Lee),정문규(Moongyu Jung),이정현(Jeong Hyun Yi) 한국정보보호학회 2016 정보보호학회논문지 Vol.26 No.1

안드로이드 애플리케이션은 악성코드를 삽입한 후 재서명하여 배포하는 리패키징 공격에 취약하다. 이러한 공격을 통해 사용자의 사생활 정보나 개인정보 유출 등의 피해가 자주 발생하고 있는 실정이다. 모든 안드로이드 애플리케이션은 사용자가 직접 작성한 메소드와 API로 구성된다. 이중 플랫폼의 리소스에 접근하며 실제 애플리케이션의 기능적인 특징을 나타내는 것은 API이고, 사용자가 작성한 메소드 역시 API를 이용하며 기능적 특징을 나타낸다. 본 논문에서는 악성 애플리케이션이 주로 활용하는 민감한 API들을 분석 대상으로 하여 악성애플리케이션이 어떤 행위를 하고, 어떤 API 를 사용하는지 사전에 식별할 수 있는 분석 기법을 제안한다. 사용하는 API를 토대로 API의 특성정보를 기반으로 나이브 베이즈 분류 기법을 적용하여 비슷한 기능을 하는 API에 대해 기계 학습하도록 한다. 이렇게 학습된 결과를 토대로 악성 애플리케이션이 주로 사용하는 API를 분류하고, 애플리케이션의 악성 위험 정도에 대한 정량적 판단 기준을 제시한다. 따라서, 제안 기법은 모바일 애플리케이션의 취약점 정도를 정량적으로 제시해 줌으로써 모바일 애플리케이션 개발자들이 앱 보안성을 사전에 파악하는데 많은 기여를 할 수 있을 것으로 기대된다. Android applications are inherently vulnerable to a repackaging attack such that malicious codes are easily inserted into an application and then resigned by the attacker. These days, it occurs often that such private or individual information is leaked. In principle, all Android applications are composed of user defined methods and APIs. As well as accessing to resources on platform, APIs play a role as a practical functional feature, and user defined methods play a role as a feature by using APIs. In this paper we propose a scheme to analyze sensitive APIs mostly used in malicious applications in terms of how malicious applications operate and which API they use. Based on the characteristics of target APIs, we accumulate the knowledge on such APIs using a machine learning scheme based on Naive Bayes algorithm. Resulting from the learned results, we are able to provide fine-grained numeric score on the degree of vulnerabilities of mobile applications. In doing so, we expect the proposed scheme will help mobile application developers identify the security level of applications in advance.

대두의 저장 단백질 Glycinin 과 그의 유전자

조태주 ( Tae Ju Cho ) 생화학분자생물학회 1992 생화학총설집 Vol.4 No.-

PCR 을 이용한 single chain Fv DNA 제조

우경남, 조태주 忠北大學校 遺傳工學硏究所 1997 遺傳工學硏究 Vol.11 No.1

-본문참고-

Characterization of a Replication Element in the Coat Protein ORF of Turnip Yellow Mosaic Virus

신현일,조태주 대한미생물학회 2012 Journal of Bacteriology and Virology Vol.42 No.1

Turnip yellow mosaic virus (TYMV) is a non-enveloped icosahedral virus that has a single 6.3 kb positive-strand RNA as a genome. Previously, it was observed that the recombinant construct TY-eGFP2, where an eGFP gene was inserted at the position downstream of the coat protein (CP) ORF of TYMV genome, barely replicated. The inhibition of replication was relieved by insertion of an additional copy of the 3' quarter of the CP ORF after the foreign sequence. In this study, we have examined if the 3' quarter of the CP ORF contains any replication elements. M-fold analysis predicted three stem-loop structures in this region. Analysis of the TY-eGFP2 constructs containing one or two of these stem-loop structures indicates that the secondary structure predicted in the region between nt-6139 and nt-6181, termed SL2, is essential for TYMV replication. The critical role of SL2 was confirmed by the observation that deletion of the 3'quarter of the CP ORF from the wild-type TYMV genome nearly abolished replication and that insertion of SL2 into the deletion mutant restored the replication. Mutations disrupting the stem of SL2 greatly reduced viral RNA replication,indicating that the secondary structure is essential for the enhancing activity.

Isolation and Characterization of Methyl Jasmonate-Inducible Genes in Chinese Cabbage

박용순,조태주 한국통합생물학회 2003 Animal cells and systems Vol.7 No.4

Methyl jasmonate (MeJA) is a signal molecule in the activation of defense responses in plants. In this study, we isolated 15 MeJA-inducible genes by subtractive hybridization. These genes encode two myrosinase-binding proteins, five lipase-like proteins, a polygalacturonase inhibitor, a putative chlorophyll-associated protein, a terpene synthase, a dehydroascorbate reductase, an ascorbate oxidase, a cysteine protease, an O-methyltransferase, and an epithiospecifier protein. Northern analysis showed that most of the Chinese cabbage genes are barely expressed in healthy leaves, but are strongly induced by MeJA treatment. We also examined whether these MeJA-inducible genes were activated by ethethon, BTH, and Pseudomonas syringae pv. tomato (Pst ), a nonhost pathogen of Chinese cabbage. The results showed that none of the MeJA-inducible genes was strongly induced by ethephon or by BTH. The genes encoding lipase-like proteins and a myrosinase-binding protein were weakly induced by Pst. Other MeJA-inducible genes were not activated at all by the pathogen.