http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Aujeszky`s Disease Virus의 실험적 감염 Rat의 병리조직학적 관찰과 면역조직화학적 항원 검출
조우영 ( Woo Young Cho ),조부제 ( Bu Je Cho ),조성환 ( Sung Whan Cho ),이철호 ( Chul Ho Lee ) 한국가축위생학회 1990 韓國家畜衛生學會誌 Vol.13 No.1
In order to investigate the clinico-pathological and immunohistochemical changes in the rats infected with Aujeszky`s disease virus(ADV), 100 heads of 4 weeks-old rats were inoculated intraperitoneally and intranasally, with the domestically isolated ADV, NYJ-1-87 strain, at 10(3.0) or 105.0TCID50/0.2m1. Results obtained through the experiments were summarized as follows: 1. Clinical signs such as dulness, anorexia, pruritus, fascial edema, dyspnea and ataxia were observed from the 2nd day and died at the 3rd to 5th day after ADV inoculation. By necropsy, congestion and hemorrhage were observed in the abdominal organs, while no specific changes were detected in the other organs. 2. In histopathological observation, degeneration and necrosis of the nervous cells, non-suppurative meningoencephalitis, microgliosis and perivascular cuffing were manifested in central nerve system but no specific changes were observed in the other organs. 3. By immunohistochemical staining using peroxidase antiperoxidase, the positive cells were detected in the tissues of kideny, spleen, urinary bladder and lung.
RT-PCR 기법을 이용한 분변내 소 코로나바이러스 검출
안재문 ( Jae Moon Ahn ),조우영 ( Woo Young Cho ),이종인 ( Jong In Lee ),조부제 ( Bu Je Cho ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.3
The reverse transcriptase polymerase chain reaction (RT-PCR) was used for the detection of bovine coronavirus (BCV) in fecal samples by using reverse transcriptase and two primers which flanked M gene sequence of 407bp. RT-PCR detected bovine coronavirus specifically, but did not detect mouse hepatitis virus (MHV), transmissible gastroenteritis virus (TGEV), and bovine rotavirus (BRV). The M gene sequences of MHV are homologus to that of BCV, but minor differences exist in the primer regions, preventing annealing of the primers. Detection of BCV using RT-PCR was compared with ELISA and the agreement of BCV detection by RT-PCR and ELISA was 95.3%. RNA detection in positive clinical specimens was significantly better by PCR than immunological detection of BCV by ELISA.