http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 조경석 ( Kyung Souk Cho ) (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
임상 : 쥐 골수 기질 세포의 면역 효과 세포로의 활성화
김효창 ( Hyo Chang Kim ),강석구 ( Seok Gu Kang ),전신수 ( Sin Soo Jeun ),유도성 ( Do Sung Yoo ),허필우 ( Pil Woo Huh ),조경석 ( Kyung Souk Cho ),김달수 ( Dal Soo Kim ),박춘근 ( Chun Kun Park ) 대한뇌종양학회 2006 대한뇌종양학회지 Vol.5 No.1
Object:Marrow stromal cells(MSCs) have been shown to have the capacity of orthodox and unorthodox plasticity. In this study, the authors tried to differentiate MSCs into immune effector cell and also to access in vitro cytotoxicity of MSCs from rat. Methods:Rat MSCs(rMSCs) were isolated by standard methodology and were activated by interleukin-2(IL-2), interleukin- 15(IL-15), colony stimulating factor GM-CSF, and combinations. Phenotypes of these various activated cells were determined using flow cytometry. The secreted protein from effector cells was estimated by enzyme-linked immunosorbent assay. Cytotoxicity of rMSCs and activated rMSCs against the target cells(9L rat glioma cell line) were estimated using visual survival cell assay. The expression of immune response related genes in activated cells was measured. Results:After various cytokines activation of rMSCs, the population of immune effector cells(CD8, CD161a) and immune reaction related proteins(IL-4, γ-INF) were increased. There was a significant cytotoxicity of rMSCs activated with various cytokine combinations. Apoptosis may be one of the lysis mechanism of target cells by activated rMSCs. The contributing genes could be γ-INF, FasL and perforin. Conclusion:This study suggests that rMSC may differentiate into immune effector cells and have cytotoxic capability against malignant glioma cells. However, we have to investigate orthotopic animal study for the proper translation.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
