http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
감염 근관에서 분리된 연쇄구균의 16S Ribosomal DNA 중합효소 연쇄반응과 제한효소 절단길이 다형성에 관한 연구
정희일,임미경 원광대학교 치의학연구소 1995 圓光齒醫學 Vol.5 No.2
Bacteria have been regarded as one of the most important factors in pulpal and periapical diseases. Streptococci are most frequently isolated facultative anaerobes in infected root canals. Recently molecular biological techniques have been rapidly progressed. This study was designed to apply the molecular biological tools to the identification and classification of streptococci in the endodontic microbiology. Streptococci isolated from infected root canals were identified with both Vitek Systems and API 20 STREP. Identification results were somewhat different in several strains of streptococci. Eighteen streptococci and enterococcal chromosomal DNA were extracted and they had two or three plasmid DNA. It was difficult to digest plasmid DNA using HindⅢ and EcoRI and to differentiate strains by restriction enzyme analysis of plasmid DNA. 16S rDNA of chromosome was amplified by polymerase chain reaction (PCR) and then restriction fragment length polymorphism (RFLP) after using several kinds of restriction enzymes was observed. The molecular mass of 16S rDNA of chromosomal DNA was approximately 1.4kb. There were three to five RFLP patterns using eight restriction enzymes. RFLP patterns digested with Cfol which recognizes four base sequences were identical in all stains. HindⅢ which recognizes six base sequences could not digest the 16S rDNA. Restriction enzymes which recognize five base sequences were suitable for RFLP pattern analysis. At least three different kinds of restriction enzymes were needed to compare each strains. It was suggested that 16S rDNA PCR-RFLP methodology was relatively simple and rapid to differentiate and classify strains and could be used in the epidemiological study of root canal infections.
정희일,임미경 원광대학교 치의학연구소 1992 圓光齒醫學 Vol.2 No.1
The purpose of this study was to investigate the antibacterial effect of zinc oxide eugenol cement (ZOE), zinc phosphate cement (ZPC), glass ionomer cement, resin, and Vitapex to Smulans, Ssanguis, Sfecalis and E.co1i by agar diffusion method. Four wells were punctured in mitis-salivarius agar plate per each group and each wells were filled with restorative materials. The width of inhibition zones produced in mitis-salivarius agar were measured as the parameter of the antibacterial effect after 16 hours and 40- hours. In Sinulans and S.sanguis, the largest inhibition zone was produced on ZOE, followed by glass ionomer cement, and ZPC. Inhibition zones was not observed in resin and Vitapex. In S.jecalis, ZOE and glass ionomer cement showed wider inhibition zone than ZPC. In E.coli, ZOE showed wider inhibition zone than ZPC, but no inhibition zone was observed on glass ionomer cement.