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미관지구내 건축선 후퇴공간의 이용에 영향을 미치는 계획방법에 관한 연구
정창구(Jung, Chang-Gu),김기수(Kim, Gi-Su) 대한건축학회 2016 대한건축학회 학술발표대회 논문집 Vol.36 No.2
Landscape environment of our country has developed by leaps and bounds and to the implementation of the green city in the heart of the pedestrian center of the vehicle is made to fit the policy realization. However, these various green policies have been proposed for the implementation of the city, the lack of extremely feasible for it came to that situation does not occur smoothly theory and practical application of the city. Among them may be causing serious administrative problems, such as the case of Seoul, the trunk space aesthetics global recession geonchukseon specified in the most horizontal of a vague report haphazard management system design, occupying the private parking lot viewed as one of the examples. Therefore, the present study was to verify the effectiveness of the improvement process as part of improving the street environment specifically derived from the census and survey the existing problems and to set the future direction corresponding to the present, empirical applications.
김태규(Tae-Gyu Kim),정창구(Chang-Koo Jung),김익진(Ik-Jin Kim),김현수(Hyun-Soo Kim) 한국기계가공학회 2007 한국기계가공학회 춘추계학술대회 논문집 Vol.2007 No.-
The artificial BN material is similar to diamond in property such as hardness, wear resistance, chemical stability and thermal conductivity. Although cutting tools made of diamond are very efficient and widely used to cut nonferrous and hard brittle materials, graphitization is a problem for their use in machining ferrous materials. However, this limitation does not exist for cubic boron nitride (CBN). Therefore, CBN is widely used in grinding and cutting applications for ferrous materials and hard-to-machining materials. In this work, CBN film was deposited on hard metal insert tool substrate by microwave plasma enhanced chemical vapor deposition method.
Lactococcus lactis subsp. lactis 유래 cyclomaltodextrinase 유전자의 대장균 내 발현 및 효소 특성
장명운 ( Myoung Uoon Jang ),강혜정 ( Hye Jeong Kang ),정창구 ( Chang Ku Jeong ),박정미 ( Jung Mi Park ),이아름 ( Ah Rum Yi ),강정현 ( Jung Hyun Kang ),이소원 ( So Won Lee ),김태집 ( Tae Jip Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2013 한국미생물·생명공학회지 Vol.41 No.4
본 연구에서 584개의 아미노산(68.7 kDa)으로 구성된 cyclomaltodextrinase (LLCD)의 유전자를 Lactococcus lactis subsp. lactis KCTC 3769 (ATCC 19435)로부터 클로닝하였다. LLCD는 일반적인 CDase 계열 효소들과 약 40% 전후의 아미노산 서열 상동성을 나타내었다. C-말단에 6개의 히스티딘 잔기를 가진 재조합 효소는 dimer의 형태로 대장균에서 발현되고 정제되었다. LLCD는 pH 7.0 및 37oC에서 최대의 β-CD 가수분해 활성을 나타내었다. 특히, 이 효소는 starch 및 pullulan에 대해 극히 낮은 활성을 보였으나, 반면에 CD에 대한 가수분해 활성은 starch에 비해 약 80배 이상 높았다. 이처럼 높은 CD에 대한 활성을 근거로 LLCD는 CDase 계열 효소로 분류될 수 있으나, starch, pullulan, 그리고 acarbose에 대한 매우 낮은 활성은 다른 유사효소와 비교하여 차별화되는 특징이다. A putative cyclomaltodextrinase (LLCD) gene was cloned from the genome of Lactococcus lactis subsp. lactis KCTC 3769 (ATCC 19435), which encodes 584 amino acids with the predicted molecular mass of 68.7 kDa. KCTC 3769 shares approximately 40% of amino acid sequence identity with the CDase-family of enzymes. The dimeric enzyme with C-terminal six-histidines was heterologously expressed and purified from recombinant E. coli. LLCD showed the highest activity against β- cyclodextrin (CD) at pH 7.0 and 37oC. In particular, LLCD exhibited extremely low activity against starch and pullulan, while its CD-hydrolyzing activity was about 80 times higher than starch. Due to its much higher activity on CD over starch, LLCD has been identified as a member of CDases. However, LLCD can be distinguished from the other common CDases on the basis of its extremely low hydrolyzing activity against starch, pullulan, and acarbose.