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정성근(Seong-Geun Jeong),이상호(Sang-Ho Lee),이창수(Chang-Soo Lee) 한국생물공학회 2013 KSBB Journal Vol.28 No.4
Herein we present a diagnostic paper chip that can quantitatively detect albumin without external electronic reader and dispensing apparatus. We fabricated a diagnostic paper chip device by printing wax barrier on the paper and wicking it with citrate buffer and tetrabromophenol blue to detect albumin in sample solution. The paper chip is so simple that we dropped a sample solution at sample pad and measure the ratio of two travel distances of the sample solvent and albumin under the name of retention factor. Our result confirmed that the retention factor was constant in the samples with same concentration of albumin and useful determinant for the measurement of albumin concentration. The paper chip is affordable and equipment-free, and close to ideal point-of-care test in accordance with the assured criteria, outlined by the World Health Organization. We assume that this diagnostic paper chip will expand the concept of colorimetric determination and provide a inexpensive diagnostic method to aging society and developing country.
온도 구배가 있는 미세유체 장치를 이용한 극지 미생물의 형태 변화 분석
정성근(Seong-Geun Jeong),박애리(Aeri Park),정헌호(Heon-Ho Jeong),홍순규(Soon Gyu Hong),이창수(Chang-Soo Lee) 한국생물공학회 2014 KSBB Journal Vol.29 No.4
We present microfluidic method to rapidly analyze the effect of temperature on the change of morphologies of Antarctic bacteria (Pseudoalteromonas sp., Shewanella vesiculosa, Shewanella sp., and Cellulophaga sp.). The microfluidic device is able to generate stable temperature gradient from 7 to 40℃ and dramatically reduce the number of experiments, experimental cost and labor, and amount of sample. Based on this approach, we found that specific bacteria transforming morphology into filament or elongated body strongly depends on cultivation temperature. Interestingly, we found that the morphologies of Pseudoalteromonas sp., Shewanella vesiculosa, Shewanella sp., and Cellulophaga sp. are elongated at below 25℃, above 20℃, above 15℃ and above 35℃, respectively. We envision the microfluidic device is a useful approach to analyze biological events with a high throughput manner.
김동호,정성근,이창수,Kim, Dong-Ho,Jeong, Seong-Geun,Lee, Chang-Soo 한국화학공학회 2020 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.58 No.2
본 연구에서는 별도의 이미지 분석 장비를 사용하지 않고 정량적으로 알부민의 농도를 측정할 수 있는 회전각도를 이용한 3차원 종이 칩(3D-PADs)를 제시한다. 변색된 구간의 회전각도를 측정하는 간단한 방법으로 검출을 시연하였다. 3D-PADs는 왁스 프린터를 이용한 인쇄와 라미네이팅 과정을 거쳐 빠르게 제작할 수 있다. 3D-PADs는 샘플의 알부민을 검출하기 위하여 citrate buffer와 tetrabromophenol blue를 흡수시켰다. 3D-PAD의 흡수패드에 샘플 용액을 흡수시키면, 샘플 용액은 형성된 유로를 통하여 수직 및 수평 흐름을 통해 분석 구간으로 흐른다. 변색된 구간의 회전각도는 특정한 알부민의 농도를 나타내며, 알부민 측정의 신뢰할 수 있는 값임을 확인할 수 있었다. This study presents an angular-based measurement on three-dimensional paper-based analytical devices (3D-PADs) for quantitative detection of albumin without using an image analyzer. We demonstrate a simple quantitative and straightforward approach based on the angle of the discolored area as detection criteria. 3D-PADs are rapidly fabricated by the wax-printing and laminating process. The 3D-PADs are treated with citrate buffer and tetrabromophenol blue to react with albumin in a sample solution. Dropping sample solution into sample pad in the 3D-PAD, fluid flows toward the assay zone laterally and vertically by capillary action. We find that the change of angle of the discolored area correctly reflects the concentration of albumin and is reliable determinant for the measurement of the albumin concentration. It is the first demonstration of angular-based detection as a simple, inexpensive, and equipment-free approach for point-of-care diagnosis.