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번데기동충하초(cordyceps militaris)와 기주가 다른 두 종의 자실체형성 차이에 관한 연구
Morphological, cultural and physiological characteristics of Cordyceps militaris, C. kyushuensis and C. chichibuensis, which were preserved in Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University, were investigated. Morphological characteristics of these three species differed from each other. C. militaris and C. kyushuensis infect and grow upon lepidopteran pupae. However, types of pupae of these two species are differed morphologically. In the case of C. chichibuensis, it grows upon lepidopteran larva. Despite their host types, morphological characteristics of stroma were similar to each other. Stromata of all three species were yellow to light orange in color. The size of stroma was 20 −45 ×1.5−3 mm, 15−28× 1.2−2.4 mm and 35−60×1.6−3.2 mm in C. militaris, C. chichibuensis, and C. kyushuensis, respectively. The size of perithecium and ascospores of all three Cordyceps species were also similar. Cultural characteristics of these three species were also similar. The optimum temperature (25?C) and pH (7.0) were found to be most favorable for the mycelial growth of all three Cordyceps species. Besides, these three species had Verticillium type conidial structure. Mating type of these three species was determined by sister crossing, out-crossing, and inter-specific crossing using their single ascospores derived isolates. Two opposite mating types were observed in both C. kyushuensis and C. chichibuensis as in C. militaris which were designated as A and a. This finding suggests that these entomopathogenic fungi are heterothallic. In order to study the genetic relationship between these three species, inter-specific mating was done. Inter-specific mating was found possible in these three species and the stromata were produced. When conidia of these three species were crossed to each other, inter-specific mating was found compatible and the stromata were produced. These results indicate that mating is compatible between C. militaris, C. chichibuensis, and C. kyushuensis and these three species are genetically close to each other.