한국재래닭의 ADSL 유전자 내 단일염기변이 탐색

오재돈,전세아,강보석,김학규,서옥석,공홍식,이학교 한국가금학회 2008 한국가금학회 정기총회 및 학술발표회 Vol.25 No.-

Adenylosuccinate lyase(ADSL) catalyzes two steps in the synthesis of purine nucleotides: (1) the conversion of succinylaminoimidazole carboxamide ribotide into aminoimidazole carboxamide ribotide and (2) the conversion of adenylosuccinate into adenosine monophosphate. An obstacle of this purine nucleotide cycle (PNC) can be caused brain functional disorder and growth disorder. So ADSL deficiency, which is associated with sever mental retardation, autistic features and energy metabolism. This study was performed to identify SNP on ADSL gene in chicken. The nucleotides were observed as T to C(7724<SUP>th</SUP> nucleotide), C to T(7732<SUP>nd</SUP> nucleotide), G to T(10108<SUP>th</SUP> nucleotide), A to T(10356<SUP>th</SUP> nucleotide), G to A(10375<SUP>th</SUP> nucleotide). A to C(10402<SUP>nd</SUP> nucleotide), A to T(12716<SUP>th</SUP> nucleotide), T to A(l2717<SUP>th</SUP> nucleotide), C to T(l5491<SUP>st</SUP> nucleotide), C to T(15542<SUP>nd</SUP> nucleotide) and C to T(l5550<SUP>th</SUP> nucleotide). The nucleotide substitutions at 15542<SUP>nd</SUP> and 15550<SUP>th</SUP> (GeneBank accession no. AY665559) were found as missense mutation (Alanine → Valine, Proline → Serine, respectively). This study will be useful for farther researches for identifying association between these SNPs and energy metabolism in chicken.

Microsatellite Marker를 사용한 재래닭 품종 식별력 및 유전적 특성 분석

김갑진,전세아,이진아,오재돈,신호연,이학교,공홍식 한국가금학회 2008 한국가금학회 정기총회 및 학술발표회 Vol.25 No.-

To estimate the genetic characteristics and cumulative power of discrimination (CPD) existing among chicken (Korean native chicken) and exotic foreign population (Korean Native Red chicken, Korean Native Black chicken, Korean Native Yellow chicken, Ogal chicken) we used a total of 195 genomic DNAs from five breeds population (Korean Native Red chicken, Korean Native Black chicken, Korean Native Yellow chicken, Ogal chicken). Genetic characteristics indices including mean allele number among loci, unbiased heterozygosity (hi) within locus and polymorphic information content(PIC) and unbiased average heterozygosity (H) among loci in four breeds were calculated using the generated allele frequencies by each marker. The generated unbiased average heterozygosity among loci in each breed was integrated to the global formula of CPD resulting in 96.91% within the populations.

Immunoglobulin E-binding Proteins of Cooked Walnuts in Korean Children

이정민,정경욱,전세아,이수영 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.4

Purpose: The immunological characteristics of young Korean children with walnut (WN) allergy and the influence of different cooking methods on WN proteins have not been evaluated to date. This study aimed to evaluate the major WN allergens identified among Korean children, together with changes in WN antigenicity caused by common cooking methods. Methods: We enrolled children under the age of 13 years with WN serumspecific immunoglobulin (Ig) E concentrations. The protein fractions of dry-fried and boiled WN extracts were compared with those of raw WNs using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), 2-dimentional gel electrophoresis (2DE) and a proteomic analysis using electrospray ionization (liquid chromatography-mass spectrometry [LC-MS]). An immunoblotting analysis was conducted to examine IgE reactivity toward raw WNs using serum samples from 6 children with a clinical WN allergy. To determine the processed WN proteins with IgE-binding capacity, a 2D-immunoblotting analysis was performed using the pooled sera of 20 WN-sensitized children. Results: Protein bands from raw WNs were identified at 9, 16, 28, 52, 58, and 64 kDa via SDS-PAGE. The 9- and 16-kDa protein bands were enhanced by boiling, whereas the 52- and 64-kDa bands were considerably diminished. On LC-MS analysis, of the 66 IgE-binding proteins present in raw WNs, 57 were found in dry-fried WNs, but only 4 in boiled WNs. The sera of 5 out of 6 participants reacted with the 52-kDa protein bands and those of 4 out of 6 participants reacted with the 16- and 28-kDa protein bands, respectively. Meanwhile, a 2D-immunoblotting result confirmed the presence of different binding patterns among children who consumed cooked WNs. Conclusions: The protein profile of boiled WNs is substantially different from that of raw WNs. However, 4 proteins including prolamins remained stable after dry-frying or boiling. Further studies are needed to evaluate the clinical relevance of these findings.

한국재래닭의 ADSL 유전자 내 단일염기변이를 이용한 경제형질과의 연관성 분석

이진아(J. A. Lee),전세아(S. A. Jeon),오재돈(J. D. Oh),박경도(K. D. Park),최강덕(K. D. Choi),전광주(G. J. Jeon),이학교(H. K. Lee),공홍식(H. S. Kong) 韓國家禽學會 2009 韓國家禽學會誌 Vol.36 No.3

소아 키위 알레르기의 임상 특성과 특이 immunoglobulin E 항체의 유용성

이정민 ( Jeong Min Lee ),전세아 ( Se Ah Jeon ),이수영 ( Soo Young Lee ) 대한소아알레르기호흡기학회 2013 Allergy Asthma & Respiratory Disease Vol.1 No.1

Purpose: Kiwi fruit allergy in children has scarcely been reported. This study focused on the clinical characteristics of kiwi fruit allergy and value of kiwi specific Immunoglobulin E (IgE) antibodies in Korean children. Methods: The study was based on a data analysis of 18 patients, who were diagnosed with clinical kiwi fruit allergy at Ajou University Hospital from June 2005 to June 2012. Clinical details were collected by medical history and telephone survey. Sera from all children were analyzed for kiwi specific IgE (ImmunoCAP) and patients with negative result were further evaluated by an enzyme linked immunosorbentassay test (ELISA) using our own made kiwi fruit extracts. Results: The subjects were 10 male and 8 female with a median aged 25 months. Twelve out of 18 (66.7%) were diagnosed with angioedema or urticaria, 4 (22.2%) were diagnosed with oral allergy syndrome, 1 was presented with dyspnea, and 1 was diagnosed with anaphylaxis. Oral route of exposure (88.9%) was most common and majority of patients (88.9%) experienced clinical symptoms at the first exposure to kiwi fruit. Six out of 12 patients with urticaria showed negative result in specific IgE by ImmunoCAP, but specific IgE were detected in 2 of 6 by ELISA with our own made kiwi extract. Conclusion: Systemic reactions to kiwi fruit were common in Korean children, and the symptoms were frequently developed at the first time of exposure. Additional diagnostic methods would be needed for evaluation of IgE sensitization in kiwi allergic patients with negative result by ImmunoCAP. (Allergy Asthma Respir Dis 1(1):73-78, 2013)

소아 키위 과일 알레르기 환자에서 성분 항원 진단의 효용성

이정민 ( Jeongmin Lee ),김혜보 ( Hyebo Kim ),정경욱 ( Kyunguk Jeong ),전세아 ( Se-ah Jeon ),이수영 ( Sooyoung Lee ) 대한천식알레르기학회 2022 Allergy Asthma & Respiratory Disease Vol.10 No.2

Purpose: In food allergy, significant component antigens can be assessed by using the microarray. The purpose of this study was to evaluate the diagnostic value of component resolved diagnosis (CRD) in young children with kiwifruit allergy. Methods: Through a retrospective review of medical records, we evaluated the clinical characteristics of 12 children who underwent measurement of serum immunoglobulin E concentrations to kiwifruit (kiwi-sigE) and were diagnosed as kiwifruit allergy. We applied ImmunoCAP ISAC-CRD 112 using the residual sera of patients at the initial visit. Results: The median age of kiwifruit allergic children was 33.5 months (range, 13 to 84 months), and the proportion of systemic reactions, including 2 anaphylaxis cases, was 66.7%. Four had localized reactions on the lips. A total of 11 (91.7%) were sensitized to Act d 1; among them, 8 were mono-sensitized to Act d 1 and 3 were sensitized to ≥2 kiwifruit components. There was no significant difference in CRD results between those with systemic reactions and those with local reactions. Conclusion: Act d 1 is the major allergenic component in Korean young children with clinical kiwifruit allergy. The additional diagnostic value of the CRD in diagnosing and predicting the severity of kiwifruit allergy is expected to be low in young children. (Allergy Asthma Respir Dis 2022;10:105-109)