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독도서식 식물근권에서 분리한 포자형성세균과 질소고정세균의 군집구조 분석
전선애,성혜리,박유미,박재홍,김사열,Jeon, Seon-Ae,Sung, Hye-Ri,Park, Yu-Mi,Pak, Jae-Hong,Ghim, Sa-Youl 한국미생물·생명공학회 2009 한국미생물·생명공학회지 Vol.24 No.3
Bacteria were isolated from roots of plants belonging to family Solanaceae and Gramineae, inhabited in Dokdo island. Fifty six endospore-forming bacteria grown on tryptic soy broth (TSB) agar medium and 23 nitrogen-fixing bacteria (NFB) grown on nitrogen free agar medium were isolated, respectively. The isolates were partially identified by analyzing the 16S rDNA and categorized into phylogenetic groups. The 16S rDNA sequences of each identified isolates were compared with sequences of each type strains to analyze phylogenetic relationship by phylogenetic tree. As a result, endospore-forming bacteria and nitrogen-fixing bacteria were classified into 4 and 6 lineage groups, respectively. Among these isolated, 18 were presumed to be novel species candidates based on the similarity (lower than 98%) analysis of the l6S rDNA sequences.