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임종환,장범수,박병권,윤효인,Lim, Jong-hwan,Jang, Beom-su,Park, Byung-kwon,Yun, Hyo-in The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.3
The objective of this study was to investigate the residue depletion of cephaalexin in the flounder (Paralichthys olivaceus) after multiple oral administrations and to establish the appropriate withdrawal time for edible tissues. A highly sensitive and specific method for the determination of cephalexin in the serum of flounder by LC/MS was developed and validated. Mean recoveries from serum were 87.2% (ranged from 81.2% to 94.5%) for cepalexin. Recovery and precision met the criteria for the guideline of residual analysis of veterinary drugs by the National Veterinary Research and Quarantine Service (NVRQS) in Korea. The limit of detection and limit of quantitation of cephalexin were 10 ng/ml and 50 ng/ml, respectively. Residual levels of cephalexin in muscle samples were estimated with 95% tolerance limit and 95% confidence to fall below the MRL after a withdrawal time of 4 days and 5 days for the 40 and 160 mg/kg/day, respectively.
Determination of Ceftiofur in Bovine Serum by liquid Chromatography-Electrospray Mass Spectrometry
임종환,장범수,박병권,윤효인,Lim, Jong-hwan,Jang, Beon-su,Park, Byung-kwon,Yun, Hyo-in The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.3
This study was aimed to develop a more rapid, simple and sensitive method to determine ceftiofur in bovine serum using LC/MS with electrospray interface. Separation was achieved on the Nova-Pak $C_{18}$ reverse phase column. The mobile phase consisted of 0.1% acetic add in water (A) and acetonitrile (B) and gradiently flowed at the rate of 0.4 mL/min. As a result of analysis of blank muscle samples, matrix interference was not shown. Limit of detection and limit of quantitaion was 5 ng/g and 20 ng/g, respectively. The values of precision and recovery satisfied the guideline of NVRQS. The precision and recovery developed in this method are suitable and sensitive to determine the concentration of ceftiofur in the bovine serum. These results could be applied for the confirmation and quantification in the biofluid.
무인모선기반 무인잠수정의 3차원 위치계측 기법에 관한 연구
임종환(JONG-HWAN LIM),강철웅(CHUL-UNG KANG),김성근(SUNG-KYUN KIM) 한국해양공학회 2005 韓國海洋工學會誌 Vol.19 No.2
A 3-D localization method of an autonomous underwater vehicle (AUV) has been developed, which can solve the limitations of the conventional localization, such as LBL or SBL that reduces the flexibility and availability of the AUV. The system is composed of a mother ship (small unmanned marine prober) on the surface of the water and an unmanned underwater vehicle in the water. The mother ship is equipped with a digital compass and a GPS for position information, and an extended Kalman filter is used for position estimation. For the localization of the AUV, we used only non-inertial sensors, such as a digital compass, a pressure sensor, a clinometer, and ultrasonic sensors. From the orientation and velocity information, a priori position of the AUV is estimated by applying the dead reckoning method. Based on the extended Kalman filter algorithm, a posteriori position of the AUV is, then, updated by using the distance between the AUV and a mother ship on the surface of the water, together with the depth information from the pressure sensor.
임종환 ( Jong Hwan Lim ),성주한 ( Joo Han Sung ),천정화 ( Jung Hwa Chun ),신만용 ( Man Yong Shin ) 한국임학회 2016 한국산림과학회지 Vol.105 No.1
본 연구는 연륜기후학적 방법에 의해 기후인자가 우리나라에 분포하는 일본잎갈나무의 연륜생장에 미치는 영향을 구명하기 위해 수행하였다. 본 연구에서는 먼저 일본잎갈나무의 연도별 생장특성을 분석하기 위해 제5차 국가산림자원자료에서 수집된 일본잎갈나무의 연륜생장 자료를 정리하였다. 한편 일본잎갈나무의 연륜생장과 기후변수와의 관계를 구명하기 위해 1950년부터 2010년까지 정리된 월평균 기온과 월강수량 자료를 시군단위로 정리하였다. 기후조건의 유사성에 근거하여 일본잎갈나무의 시군별 연륜생장 자료를 군집분석한 결과 6 개의 기후 군집으로 구분할 수 있었다. 이와 함께 연륜생장 자료에 대한 크로스데이팅과 표준화 절차를 통해각 군집별 지표연대기를 제작하였다. 각 군집별 지표연대기의 적합성은 연륜생장의 평균민감도, 자기상관계수,신호 대 잡음비, 신호강도와 같은 기초통계량 분석을 통해 점검하였다. 최종적으로 각 군집별 일본잎갈나무의 연륜생장과 기후변수 간의 관계를 구명하기 위해 반응함수 분석을 실시하였다. 본 연구에서 얻어진 결과는 일본잎갈나무의 지역별 생장특성의 추정뿐만 아니라 기후변화에 따른 생장패턴의 변화 예측에 필요한 유용한 정보로 활용될 수 있을 것으로 기대된다. This study was conducted to analyze the effect of climatic variables on tree-ring growth of Larix leptolepis distributed in Korea by dendroclimatological method. For this, annual tree-ring growth data of Larix leptolepis collected by the 5th National Forest Inventory were first organized to analyze yearly growth patterns of the species. To explain the relationship between tree-ring growth of Larix leptolepis and climatic variables, monthly temperature and precipitation data from 1950 to 2010 were compared with treering growth data for each county. When tree-ring growth data were analyzed through cluster analysis based on similarity of climatic conditions, six clusters were identified. In addition, index chronology of Larix leptolepis for each cluster was produced through cross-dating and standardization procedures. The adequacy of index chronologies was tested using basic statistics such as mean sensitivity, auto correlation, signal to noise ratio, and expressed population signal of annual tree-ring growth. Response function analysis was finally conducted to reveal the relationship between tree-ring growth and climatic variables for each cluster. The results of this study are expected to provide valuable information necessary for estimating local growth characteristics of Larix leptolepis and for predicting changes in tree growth patterns caused by climate change.