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Bovine Corneal Opacity Assay법을 이용한 안점막 자극 물질의 검증에 관한 연구
이현경 ( Hyun Kyoung Lee ),노경진 ( Kyoung Jin Noh ),석승혁 ( Seoung Hyeok Seok ),백민원 ( Min Won Baek ),이희영 ( Hui Young Lee ),김동재 ( Dong Jae Kim ),나이랑 ( Yi Rang Na ),박성훈 ( Sung Hoon Park ),김배환 ( Bae Hwan Kim ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.2
Bovine corneal opacity and permeability assay have been proposed as reliable alternatives to the Draize test. In this study, we measured the opacity of the bovine cornea by quantifying the ability of light to pass through it using opacitometer. We obtained the regular value of basal opacity using bovine cornea collected within three hours after killing. For opacity alone, values obtained for 3 chemicals that are already known as irritants showed a correlation of r=0.994 (p=0.001) with Draize score about same materials. Comparing to reference Draize data from ECVAM (European Centre for the Validation of Alternative Methods), a correlation coefficient with our Draize score for the 3 chemicals was 0.982 (p=0.003). Bovine cornea test data of additional 3 chemicals already known as irritants showed a correlation of r=0.968 (p<0.001) with reference Draize data from ECVAM. Then the bovine cornea opacity test and Draize test with 3 cosmetics were performed. In both tests, we obtained similar results that showed all cosmetics were non-irritant. These results indicate that bovine corneal opacity assay has the potentiality to be developed as alternatives to rabbit eye irritation test.
안점막 자극시험 대체시험법으로서 neutral red uptake assay 확립
이현경 ( Hyun Kyoung Lee ),노경진 ( Kyoung Jin Noh ),석승혁 ( Seung Hyeok Seok ),백민원 ( Min Won Baek ),이희영 ( Hui Young Lee ),김동재 ( Dong Jae Kim ),나이랑 ( Yi Rang Na ),박성훈 ( Sung Hoon Park ),김배환 ( Bae Hwan Kim ),박 한국동물실험대체법학회 2008 동물실험대체법학회지 Vol.2 No.1
The necessity of using animals to test whether new chemicals and products are eye irritants has been questioned over the last 20 years. During this process, numerous non animal methods have been proposed as reliable alternatives to the Draize test. The neutral red uptake assay is the in vitro method to test the eye irritation of chemicals and products. We need to validate the neutral red uptake assay as alternatives for the international harmonization. Therefore, we studied for the establishment of the neutral red uptake assay in Korea. In this study, we evaluated the eye irritation of five chemicals and three cosmetics by the Draize test and the neutral red uptake assay. The results of the neutral red uptake assay showed a correlation of r=0.893(p=0.107) with our Draize score in two chemicals. And the results of the neutral red uptake assay showed correlation of r=0.892(p=0.017) with the Draize score obtained from the ECVAM (European Centre for the Validation of Alternative Methods) in additional three chemicals. All of three cosmetics were evaluated as non irritants by our Draize test and the neutral red uptake assay. As a result, the neutral red uptake assay was established in Korea. For the international harmonization, the validation study has to be performed with more chemicals and products in more countries.
연구논문 : Bovine Corneal Opacity Assay법을 이용한 안점막 자극 물질의 검증에 관한 연구
이현경 ( Hyun Kyoung Lee ),노경진 ( Kyoung Jin Noh ),석승혁 ( Seoung Hyeok Seok ),백민원 ( Min Won Baek ),이희영 ( Hui Young Lee ),김동재 ( Dong Jae Kim ),나이랑 ( Yi Rang Na ),박성훈 ( Sung Hoon Park ),김배환 ( Bae Hwan Kim ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.2
Bovine corneal opacity and permeability assay have been proposed as reliable alternatives to the Draize test. In this study, we measured the opacity of the bovine cornea by quantifying the ability of light to pass through it using opacitometer. We obtained the regular value of basal opacity using bovine cornea collected within three hours after killing. For opacity alone, values obtained for 3 chemicals that are already known as irritants showed a correlation of r=0.994 (p=0.001) with Draize score about same materials. Comparing to reference Draize data from ECVAM (European Centre for the Validation of Alternative Methods), a correlation coefficient with our Draize score for the 3 chemicals was 0.982 (p=0.003). Bovine cornea test data of additional 3 chemicals already known as irritants showed a correlation of r=0.968 (p<0.001) with reference Draize data from ECVAM. Then the bovine cornea opacity test and Draize test with 3 cosmetics were performed. In both tests, we obtained similar results that showed all cosmetics were non-irritant. These results indicate that bovine corneal opacity assay has the potentiality to be developed as alternatives to rabbit eye irritation test.
연구논문 : 안점막자극시험의 대체시험법으로서 bovine corneal opacity assay의 확립
노경진 ( Kyoung Jin Noh ),석승혁 ( Seoung Hyeok Seok ),백민원 ( Min Won Baek ),이영희 ( Young Hui Lee ),김동재 ( Dong Jae Kim ),나이랑 ( Yi Rang Na ),박성훈 ( Sung Hoon Park ),이현경 ( Hyun Kyoung Lee ),김배환 ( Bae Hwan Kim ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.1
The objective of this study is establishing of an alternative method using bovine cornea to rabbit eye irritation test. The bovine corneal opacity and permeability assay is checking opacity and permeability of bovine cornea after exposure of chemical irritants. In this study, we measured the opacity of the bovine cornea by quantifying the ability of light to pass through it using opacitometer. In addition, we optimized the basal opacity level before treatment of irritants to bovine corneas. With these results, we established the standard operating procedure of bovine corneal opacity assay.
안점막자극시험의 대체시험법으로서 bovine corneal opacity assay의 확립
노경진 ( Kyoung Jin Noh ),석승혁 ( Seoung Hyeok Seok ),백민원 ( Min Won Baek ),이영희 ( Young Hui Lee ),김동재 ( Dong Jae Kim ),나이랑 ( Yi Rang Na ),박성훈 ( Sung Hoon Park ),이현경 ( Hyun Kyoung Lee ),김배환 ( Bae Hwan Kim ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.1
The objective of this study is establishing of an alternative method using bovine cornea to rabbit eye irritation test. The bovine corneal opacity and permeability assay is checking opacity and permeability of bovine cornea after exposure of chemical irritants. In this study, we measured the opacity of the bovine cornea by quantifying the ability of light to pass through it using opacitometer. In addition, we optimized the basal opacity level before treatment of irritants to bovine corneas. With these results, we established the standard operating procedure of bovine corneal opacity assay.
안점막 자극시험의 대체법으로서의 porcine corneal opacity 및 permeability assay
한주희 ( Ju Hee Han ),이현경 ( Hyun Kyoung Lee ),석승혁 ( Seung Hyeok Seok ),백민원 ( Min Won Baek ),이희영 ( Hui Young Lee ),김동재 ( Dong Jae Kim ),나이랑 ( Yi Rang Na ),박성훈 ( Sung Hoon Park ),김배환 ( Bae Hwan Kim ),박귀례 ( 한국동물실험대체법학회 2008 동물실험대체법학회지 Vol.2 No.1
The Draize eye irritation test has been changed to alternative methods based on 3 Rs (Replacement, Reduction, Refinement) of animal experiments. The bovine corneal opacity and permeability (BCOP) assay is well established as one of the alternative methods to the Draize test but the supply of bovine eyes from a slaughterhouse is not sufficient in actuality. The porcine corneal opacity and permeability(PCOP) assay have been proposed as a reliable alternative to the BCOP assay and the Draize test because porcine corneas have numerous advantages including structural similarities with human corneas and the stable capacity for a corneal supply. In this study, three cosmetics and seven chemicals that are already known as irritants at Draize score and the bovine corneal opacity(BCO) assay were tested by the PCOP assay for the first time in Korea. The porcine corneal opacity was measured by quantifying the ability of light to pass through a cornea using an opacitometer. The permeability was measured at 10 and 30 min time points after chemical exposure. The PCOP assay showed the high correlation with the Draize score and BCO assay. These results showed that the PCOP assay can be used as an alternative method for the BCO assay and the Draize test although it needs further studies about insoluble chemicals and others to increase reliability.