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백굴채약침액(白屈菜藥針液)이 LPS로 유도(誘導)된 RAW 264.7 대식세포(大食細胞)에서의 항염증효과(抗炎症效果)
박동천 ( Dong Cheon Park ),박지현 ( Ji Hyeon Park ),이우경 ( U Kyung Lee ),이진규 ( Jin Kyu Leek ),서일복 ( Il Bok Seo ),김호현 ( Ho Hyun Kim ),김정선 ( Jeong Seon Kim ),김이화 ( Ee Hwa Kim ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.2
Objectives: Recently, Herbal-acupuncture therapeutics has been used for the treatment of inflammatory diseases such as rheumatoid arthritis. Especially, we have been interested in chemical mediators concerned with inflammation such as prostaglandin, cytokine, nitrous oxide. The purpose of this study is investigated that the effect of Chelidonii Herbal-acupuncture solution in lipopolysaccharide-stimulated RAW 264.7 macrophages, performed several expeimental items: those are prostaglandin E2, nitric oxide and cyclooxygenase-2. Methods: The cytotoxicity of Chelidonii Herbal-acupuncture solution in RAW 264.7 macrophages were measured by MTT-based cytotoxicity assay. In order to observe cyclooxygenase-2 mRNA expression in lipopolysaccharide-stimulated RAW 264.7 macrophages, RT-PCR was used. Prostaglandin E2 formation and nitric oxide production was measured by competitive enzyme immunoassay and Griess assay. Results: 1. The MTT assay demonstrated that cytotoxic effect of Chelidonii Herbal-acupuncture solution in RAW 264.7 macrophages were not appeared before concentration of 1㎎/㎖. 2. Chelidonii Herbal-acupuncture solution inhibited cyclooxygenase-2 mRNA expression in lipopolysaccharide-stimulated RAW 264.7 macrophages. 3. Chelidonii Herbal-acupuncture solution inhibited nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 macrophages. 4. Chelidonii Herbal-acupuncture solution inhibited prostaglandin E2 formation in lipopolysaccharide-stimulated RAW 264.7 macrophages. Conclusions: On the basis of these results, It was shown that Chelidonii Herbal-acupuncture solution is significantly able to inhibit the production of PGE2 and NO, as well as COX-2 mRNA expression. Our results may provide new mechanism by which Chelidonii Herbal-acupuncture solution accounts for its beneficial effect on accelerating wound healing and anti-inflammation.
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