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EDDS가 바이오에너지 작물의 중금속 흡수에 미치는 영향
이중헌,성기준,Lee, Junghun,Sung, Kijune 한국지하수토양환경학회 2015 지하수토양환경 Vol.20 No.4
Plants grown in metal-contaminated sites have to be managed and disposed of safely even in phytoremediation because heavy metals can be transferred to other organisms through the food chain, which could result in bioaccumulation in organisms of a higher trophic level. However, if the harvested plants could be used for bioenergy, the ecological risk is reduced and phytoremediation improves economic feasibility. This study researched the effects of EDDS (Ethylenediamine disuccinate) on the heavy metal uptake performance of Brassica campetris and Sorghum biocolor, both of which have potential as bioenergy plants. The results showed that EDDS could increase Pb, Cu, Ni, Cd, and Zn concentrations in the roots and shoots of both of these plants. Furthermore, EDDS reduced the metal inhibition of the S. bicolor length growth. The translocation factors (TF) of S. bicolor and B. campestris are smaller than one for all five heavy metals tested and decreased by the following order: heavy metal + EDDS > heavy metals only > uncontaminated soil. The results suggest that with regard to plant growth and metal accumulation, S. bicolor treated with EDDS is more suitable than is B. campestris for the phytoremediation of soils contaminated with multiple metal species.
폴리머 기판 위의 나노 스케일 금속 박막의 탄성계수 평가에 대한 연구
최현주(Hyun-ju Choi),김재현(Jae-Hyun Kim),이희정(Hee-jung Lee),이중헌(Joong-Hun Lee),김경식(Kyung-Sik Kim),이학주(Hak-Joo Lee) 대한기계학회 2007 대한기계학회 춘추학술대회 Vol.2007 No.10
Nano-scale metal thin films have been extensively utilized in many fields including semiconductor, MEMS, display, and bio-medical applications. Elastic modulus of a metal thin film is a basic parameter for designing a device utilizing it, but it is difficult to measure the elastic modulus of the film when it’s characteristic size is nano-scale. When a stiff thin film is compressively stressed on a soft polymer substrate, it usually forms a wrinkled pattern with a characteristic wavelength due to buckling. The wavelength is closely related to the elastic moduli of the thin film and the substrate. In this study, residually-compressed Au and Ti thin films are deposited on thick PDMS(polydimethylsiloxane) substrates and the resulting wrinkled patterns are analyzed using white light interferometer. The elastic moduli of the thin films are esi mated based on the analysis of the wrinkled patterns and the estimated elastic moduli are compared with those measured by micro tensile test.
연속 발효조를 이용한 soluble glucan 생산 공정 개발
문찬준,이중헌,Moon, Chan-Jun,Lee, Jung-Heon 한국생명과학회 2006 생명과학회지 Vol.16 No.6
Continuous fermentation process for the production of soluble glucan using mutant Agrobacterium sp. ATCC31750 has been developed in this research. When the concentration of soluble glucan was higher than 6 g/l, the viscosity of the fermented broth was too high and it needs complex separation process to separate from culture broth. Mathematical models which describe the cell growth and glucan production was developed and they kinetic parameters were estimated with experimental data. They are used for the optimization of continuous fermentation process and calculate optimal dilution rate for easy separation of glucan 4 g/l. With continuous fermentation, glucan production rate was increased 1.8 times more than that with batch fermentation.
(α-Amylase가 고정화된 Magnetic Nanofiber를 이용한 전분 분해공정 개발
김현,이중헌,Kim, Hyun,Lee, Jung-Heon 한국생명과학회 2007 생명과학회지 Vol.17 No.9
자석으로 분리가 가능한 ${\alpha}-Amylase$가 코팅된 나노고분자를 제조하여 녹말의 분해공정에 활용하였다. 본 연구에서 개발된 고정화 효소의 안정성은 크게 증가하여 상온에서 200rpm으로 교반하면서 보관한 경우 30일 동안에 92.7%의 활성도를 유지하였다 . 고정화 효소를 자석을 이용하여 재사용한 경우 10 회 동안 사용했을 경우 95.2%의 활성도 회수율을 보 여 효소의 재사용 가능성을 확인시켜 주었다 . 고정화 효소 0.5mg을 사용하여 녹말 분해 공정 에 활용하였을 때 2 ml의 40 g/l 녹말 용액을 40분만에 완전히 분해 시켰다, 이러한 고정화 효소를 사용하여 연속 효소반응기를 개발하여 녹말 분해공정에 활용한 결과 체류시간을 1시간으로 하였을 때 녹말 30 g/l를 76% 분해시켜 산업적으로 활용 가능성을 보여 주었다. Magnetically separable enzyme-coated nanofibers were developed for the hydrolysis of starch. Stability of ${\alpha}-amylase-coated$ nanofiber was greatly improved and its residual activity was maintained over 92.7% after 32 days incubation at room temperature and under shaking conditions (200 rpm). The recovery of enzyme was high and enzyme activity after 10 recycle was 95.2% of its original activity. Developed enzyme-coated nanofibers were used for the hydrolysis of starch. When 0.5 mg of magnetically separable enzyme nanofibers was used, 40 g/l of starch (2 ml) was completely degraded within 40 min. The continuous enzyme reactor was developed and used for starch hydrolysis and 76% of starch (30 g/l) was hydrolyzed with 1 hr residence time.
A5052-H112 합금의 겹치기 마찰교반접합 조직 특성
고영봉,이중헌,박경채,Ko, Young-Bong,Lee, Joong-Hun,Park, Kyeung-Chae 대한용접접합학회 2009 대한용접·접합학회지 Vol.27 No.6
The Friction Stir Welding(FSW) has mainly been used for making butt joints in Al alloys. Development of Friction Stir Lap Welding(FSLW) would expand the number of applications. Microstructure of FSLW in A5052-H112 alloy was investigated under varying rotation and welding speed. As the rotation speed was increased and the welding speed was decreased, a amount of heat was increased. As a result, bead interval was narrower, bead width are larger, and experimental bead interval was almost similar to theoretical bead interval. Typical microstructures of FSLW A5052-H112 alloy consist of three zones, including Stir Zone(SZ), Thermo-Mechanically Affected Zone(TMAZ) and Heat Affected Zone(HAZ). As a amount of heat was increased, average grain size was larger in three zones. Nevertheless, the aspect ratio was almost fixed for FSLW conditions. The misorientation of SZ, HAZ and TMAZ was examined. A large number of low angle grain boundaries, which were formed by severe plastic deformation, were showed in TMAZ as comparison with SZ and HAZ. Microhardness distribution was high in order of BM, SZ, TMAZ, and HAZ. The Micro-hardness distribution in HAZ, TMAZ of upper plate were lager than lower plate. Relationship between average grain size and microhardness was almost corresponded to Hall-Petch equation.