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Xylose isomerase 유전자를 클로닝한 알콜 발효성 효모의 개발:대장균의 xylose isomerase 유전자의 클로닝
이인구,서정훈,박우철,박희동,조진기,배신철 경북대학교 유전공학연구소 1986 遺傳工學硏究所報 Vol.1 No.1
For the purpose of cloning of Escherichia coli D-xylose isomerase (D-xylose ketol-isomerase, EC 5.3.1.5) gene to Saccharomyces cerevisiae, a 12.9Kb DNA fragment of Hind Ⅲ-digested chromosomal DNA of E. coli, containing genes for D-xylose utilization was inserted into the Hind Ⅲ site of YEp13, a shuttle plasmid between E. coli and S. cerevisiae. This recombinant plasmid, pEXI, could genetically complemented various kinds of D-xylose negative mutant in E. coli. The 12.9Kb DNA fragment of pEXI contained not only xylA and xylB genes but also xylR/T gene. The 1.6Kb DNA fragment of Bgl Ⅱ-digested pEXI was isolated and subcloned into Bam HI-digested or Bgl Ⅱ-digested YEp13 plasmid. The 1.6Kb DNA fragment of plasmid contained structural gene and promotor for D-xylose isomerase. Transformant of pEXI in E. coli produced D-xylose isomerase 3.0 times as much as parent strain.