심상성 천포창 환자에서 자가항체 검출을 위한 간접면역형광법에 기질이 미치는 영향

이상진(Sang Chin Lee),이준영(Jun Young Lee),김영환(Yung Hwan Kim),김정원(Chung Won Kim) 대한피부과학회 1993 大韓皮膚科學會誌 Vol.31 No.4

Background : Substrates play an important role in the effectiveness of the indirect immunofluro-rescence test. The monkey eaophagus has been known to be a more reliable and reproducible substrate than any other animal substrates for detecting pemphigus vulgaris autoantibodies, but it is quite difficult to get the monkey esophagus in Korea, therefore, we tried to find other substrates which could be substituted for it. Objective : We studied to select the substrate which can be the most sensitive, to compare the immunofluorescent sensitivity of various animal substrates and fores,cins for detection of pemphigus autoantibodies, and finally to evaluate the effectiveness of cultured hunan keratinocytes as a substrate for indirect immunofluorescerice. Method : With foreskin, guinea pigs, cats, rats, dogs, rabbits and cultured human keratinocytes as substrates, an indirect imrnunofluorescence test was performed to detect pemphigus vulgaris autoantibodies in seven sera from the active patients before treatment and diluted serially from 1:10 to 1:1280 Result : We could detect pernphigus autoantibodies in all animal subtrates used in this study except rats and obtain good immunofluorescent results with foreskin, guinea pigs, and cultured human keratinocytes. The results were moderate with dogs, cats, rabbits, and poor with rats. Conclusion : We confirm thut cultured human keratinocytes can be a good effective substrate. According to effectiveness, subtrates can be divided into three groups. Foreskin, guinea pigs, and cultured human keratinocytes are included in the good effective substrate group, dogs, cats, and rabbits in the modeate group and rats in the poor substrate group, but we can not find any superiority of substrates within each groups. (Kor J Dermatol 1993;31(4):7474-480)

영아 둔부 육아종

이상진(Sang Chin Lee),최성우(Sung Woo Choi),이증훈(Jeung Hoon Lee) 대한피부과학회 1993 대한피부과학회지 Vol.31 No.2

Granuloma gluteale infantum may be considered a complication oi the primary irritant type of diaper dermatitis and a modern disease due to the change in tae are of the napkin area. Most patients were infants who had worn plastic diapers and were treated with fluorinated topical corticasteroid cream for their diaper dermatitis. The lesions comorise one or several, soft and reddish brown or livid purple nodules on the napkin area. We report a typical case of granuloma gluteale infantum in a 13-month old female who had been treated with topical cortieosteroid crearn for her diaper derniat;lis. The granuloma regressed spontaneously after withdrawl of the topical corticosteroid and restricted use of the occlusive plastic diaper. (Kor J Dermatol 1093; 31(2): 228-231)

MIP-1 α cause Leukocyte recruitment into human skin

이상진 ( Sang Chin Lee ) 대한피부과학회 1999 초록집 Vol.37 No.20

경기도 부천시 국민학교 1학년 아동들의 아토피 피부염의 유병율과 동반된 보조 피부증상의 빈도

이상진(Sang Chin Lee),변대규(Dae Gyoo Byun),김진우(Won Chul Lee),이원철(Jin Wou Kim) 대한피부과학회 1995 대한피부과학회지 Vol.33 No.1

Bsckground : The incidence of atopic dermatitis(AD) varie widely by different ages and it has been well-founded that the epidemiology of AD has been studied on admission of the first grade schoolchildren. AD is a common skin.disease of childhood and tends to increase significantly during recent decacles, but informations regarding the eact prevalence of AD, based on the large-scale general population study, are unavailable in Korea. Objective : The purpose of this study is to elucidate the prevalence of AD and to evaluate the diagnostic significance of some well-known clinical features associated with AD in the first grade school children in one of Korean citie Puchon, Kyunggi-do. Methods : Four thousands and eighteen first grde school children(boys; 2072, girls; 1946) participated in this study. Two dermatologists determined presence of skin lesions by through skin examination. Diagnosis of AD was made only who typical flexural and/or extensor and/or infraauricular fissure/eczema exist. The frequny as well as their diagnostic significance of ten minor features of AD(xerosis, ichthyosifor skin lesion, hyperlinear palm, keratosis pilaris, non-specific hand/foot dermatitis, cheilitis, ple eczema, perifollicular accentuation, pityriasis alba, Dennie-Morgan infraorbital fold) thught suitable items for field survey by authors, were also evaluated. Results : The total prevalence of AD in the first grade school children of Puchon city was 3.78%. The prevalence in boys was 3.37% and that in girls was 4.1%. Associated minor features in individuals diagnosed as having AD were, in order of frqiency, pityriasis alba(59.8%), perifollicular accentuation(57.8%), keratosis pilaris(56.5%), hyprlinear palms(55.2%), xerosis (43.3%), Dennie-Morgan infraorbital fold(26.3%), etc. All bu the ichthyosiform skin lesions and nipple eczema were significantly more frequent in indivulu, 1s diagnosed as having AD(P<0.01). Conclusion : A relatively low prevalence shown in this stud right be due to arather strict diagnostic criteria. Some belpful and diagnoktically significan tumor clinical features of AD, suitable for field survey suggested. Further studies are need for accurate estimation of the prevalence of AD in Korea including different regional and population. (Kor J Dermatol 1995; 33(1): 1-7)

융기성 피부섬유육종 3예

신현민,김시용,이상진,한기택,김정원 ( Hyun Min Shin,Si Yong Kim,Sang Chin Lee,Ki Taek Han,Chung Won Kim ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.2

Dermatofibrosarcoma protuberans(DFSP) is a rare, distinctive cutaneous tumor, which consists of spindle shaped ceils arranged in densely packed interlacing bundles with the storiform or cartwheel pattern. Histologically, it resembles deep growing dermatofibroma, nodular fasciitis, neurofibroma and neural sheath tumors. DFSP is one of t.he connective tissue tumors which is difficult. to diagnose histologically as well as clinically. Recently, the immunochemical staining with a monoclonal antibody to CD34 is reported to give assistance in the clear differential diagnosis of DFSP from other fibrous or neural tumors. Herein, three cases of DFSP were stained by immunohistochemical staining with S-100 protein, vimentin, factor VIII and anti-CD34 antibody in order to assess the use of anti-CD34 in the differential diagnosis of DFSP. (Kor J Dermatol 1997;35(2): 327-332)

옻나무에 의한 알레르기성 접촉피부염과 경구복용에 의한 전신성 접촉피부염의 면역조직화학적 비교

정소희,조상현,이상진,김시용 ( So Hee Jeong,Sang Hyun Cho,Sang Chin Lee,Si Yong Kim ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.6

Background: The rhus has been known to be such a potent allergen that it causes severe contact dermatitis, and mary studies have tried to reveal the mechanism of allergic contact. dermatitis to urushiol. However, the mechanism of systemic contact dermatitis to urushiol is not, well-known, though the consumption of rhus as a health product and for desensitization is not unconmon in Korea. Objective : The purpose of this study was to evaluate the niechanism of systernic contact dermatis, and we carried out irnmunohistochemical stainings un the two strands of dermatitis (systemic arid,llergic contact dermatitis). Methods : The patients of systemir, contact dermatitis were divided into three groups by duration and severity of the illness according to the desired criteria. We perfor med immunohistor,hernical staining of the tissues from the pat.ients with anti CD4, CD8, CDla, ICAM-1, II,-ZR monoclorial antibodies. Results : 1. The results were as follows. 1. The immunohistochemical staining showed that CD8 positive cell numbers in systemic contact dermatitis(18 -L 1 0) were significantly lower than those of allergic contact der matitis(37 +- 13) ( p< 0.05). 2. The expressions of HLA-DR and ICAM-1 in the keratinocytes were not significantly different. between systemic and allergic contact derrnatitis(p>0.05). Conclusion : The result suggest that lower numbers of CDH positive T cell in systemic contact dermatitis caused a reduation in the suppressor effect to the urushiol mediated immune rection and CD8 positive T cells play an important role in systemic contact dermatitis due to rhus. (Korean J Dermatol 1997;35(6): 1052-1058)

포도구균 Toxic Shock Syndrome Toxin - 1이 아토피 피부염 환자의 말초혈액 단핵구의 Interleukin과 Interferon - gamma 분비에 미치는 영향

양종규 ( Jong Kyu Yang ),이상진 ( Sang Chin Lee ),김시용 ( Si Yong Kim ),김정원 ( Chung Won Kim ) 대한피부과학회 1998 大韓皮膚科學會誌 Vol.36 No.3

Background: The majority of Staphylococcus aureus strains isolated from atopic eczema skin produce toxins like enterotoxin B or toxic shock syndrome toxin-1(TSST-1) as a superantigen and these toxins may contribute to the exacerbation of this skin disease. Objective :We designed this study in order to investigate the effect of toxic shock syndrome toxin-1 on IL-4, interleukin-10(IL-10), interleukin-12(IL-12) and IFN- r production by peripheral blood mononuclear cells from normal and atopic patients. Methods : Peripheral mononuclear cells obtained from ten patients with severe atopic dermatitis and ten healthy volunteers were stimulated with TSST-1 and cultured for 4 days. The amount of IL-4, IL-10, IL-12 and IFN- r in the culture supernatants were determined by a solid-phase enzyme- linked immunosorbant assay. Results: l. After stimulation with TSST-1, IL-4, IL-10, IL-12 and INF- r, production of peripheral mononuclear cells from both atopic patients and healthy volunteers were increased. 2. After stimulation with TSST-1, the amount of IFN- r in the culture supernatants was significantly greater in the healthy controls than in the atopic patients. 3. After stimulation with TSST-1, the amount of IL-10 in the culture supernatants was significantly greater in the healthy controls than in the atopic patients. Conelusion : This data indicates that TSST-1 induces Thl-type and Th2-type cytokines associated with parallel Thl and Th2 stimulation in atopic patients and healthy donors. In addition, peripheral mononuclear cells from atopic patients have a diminished capacity to synthesize IFN- r and IL-10 in vitro in response to stimulation with TSST-1. Our results suggest that TSST-1 is not considered as a really important aggravating factor in atopic dermatitis. (Korean J Dermatol 1998;36(3): 385-390)

건과 건막의 투명세포육종

정소희,신현민,이동원,이상진,김시용,조백기 ( So Hee Jeong,Hyun Min Shin,Dong Won Lee,Sang Chin Lee,Si Yong Kim,Baik Kee Cho ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.2

Clear cell sarcoma of the tendon and aponeurosis was first described by Enzinger in 1965, and has become a well-accepted clinicopathological entity as malignant melanoma of soft parts. Although it posesses the ability to produce melanin, it is quite different from conventional melanoma in that, it is more deeply located, usually associated with tendon and aponeurosis, displays pale-staining round to oval-shaped tumor cells and has a longer clinical course and better prognosis.Several cases have been reported in Korea, and we experiened an additional case of this rare tumor that showed ulceration. (Kor J Dermatol 1997;35(2): 392-395)

피부로 전이된 방광암 1예

김시용,이상진,장인강,박기완 대한피부과학회 2000 大韓皮膚科學會誌 Vol.38 No.4

Metastases to the skin from internal malignancy are very rare and have been reported to occur in 0.9% to 8% of patients with cancer. Particularly there are few cutaneous metastatic carcinomas from the urinary bladder and their incidence is 1% to 2% of the cutaneous metastasis. A 76-year-old man developed cutaneous metastasis six months later after the diagnosis of carcinoma of the bladder had been made. He was presented with asymptomatic erythematous indurated plaques on the left thigh and the inguinal area. This lesion was diagnosed histologically as poorly differentiated transitional cell carcinoma. We report a case of cutaneous metastasis from carcinoma of the urinary bladder showing the unusual clinical feature of an erythematous indurated plaque.

Spitz모반이 동반된 다점성 흑자모반 1예

우혜진,김시용,이상진 대한피부과학회 2000 大韓皮膚科學會誌 Vol.38 No.12

Speckled lentiginous nevus(SLN) is characterized by a tan background patch on which more darkly pigmented macules and papules are randomly distributed. The presence of agminated Spitz nevi with SLN is well known, but a solitary Spitz nevus arising on a SLN is rare. We herein report a case of SLN with two Spitz nevi developed individually and congenital melanocytic nevus in a 9-year-old boy.