리그닌의 응용 (제1보). 활성탄 제조 및 성능조사

박병각,이병근,이갑용,Byung Kak Park,Byung Guen Lee,Gab Yong Lee 대한화학회 1976 대한화학회지 Vol.20 No.2

1기압하에서 한국산 소나무로부터 lignin을 추출할 수 있는 실험실에서의 최적 조건을 구했고, m.p, 증색반응, IR등의 방법으로 순수한 lignin임을 확인했다. 추출한 순수 lignin을 진한 황산으로 탄화한 후 전기로 속에서 650$^{\circ)C$로 공기 부활하여 활성탄을 제조하였다. 이 활성탄의 광전 비색계에 의한 흡착능을 조사한 결과 89%였다. The purpose of this paper is to use lignin. Lignin was obtained by digestion of Korean pine wood, purified, and identified. After carbonizing lignin by con $c-H_2SO_4$, the resulting carbon was converted into active carbon by activating it in the electric kiln. The adsorptivity of this active carbon was obtained by measurement with a spectro-photometer. It has been found as a result that adsorption power is 89%.

Chemical Properties of Slow-Released Nitrogen Fertilizer Using Waste Paper Slurry

김복진,백준호,이병근,Kim, Bok-Jin,Back, Jun-Ho,Lee, Byung-Guen 한국토양비료학회 2000 한국토양비료학회지 Vol.33 No.2

폐지섬유 내로 요소를 침적시켜 폐지섬유 내에 침투된 요소성분이 토양 중에서 서서히 용출됨으로써 작물의 요구에 알맞게 양분을 서서히 공급함과 동시에 이들 폐지섬유도 분해되도록 개발된 완효성 요소비료(시제품)의 화학적 특성, 미세구조 및 수중 질소용출량을 조사한 바 얻어진 결과를 요약하면 시제품의 화학적 조성은 질소가 26%, 인산, 가리는 각각 0.04. 0.01%였으며, 함유된 유해성분들 중에 크롬, 구리 납 등은 검출되었지만 비료공정규격 이하였으며, 비소와 카드뮴은 검출되지 않았다. 폐지섬유와 시제품의 구조를 주사전자 현미경(SEM)으로 확대해 보면 폐지섬유의 내부에는 빈 공간이 보이지만, 시제품에서는 폐지섬유의 셀룰로오스성 -OH기에 극성의 요소성분이 달라붙어 있었으며, 세포벽에 요소가 침적된 모습이 보인다. 시제품의 수중 질소용출량은 12시간까지는 60.4%로 급격하게 용출량이 많았으나, 그 후 서서히 용출되어 72시간에 75%까지 용출되었다. The purpose of this study was conducted to develop the slow-released N fertilizer(SRNF) using of waste paper cellulose. Properties of trial product was investigated. Contents of nitrogen, phosphorus, and potassium in trial product were showed 26%, 0.04 and 0.01%, respectively. The contents of Cr, Cu, Pb and Zn were showed 17.4ppm. 259ppm, 12.2ppm and 60.0ppm in the trial product, respectively. However, As and Cd was not detected. Nitrogen of SRNF could be released 60.4% within 12hr after dissolution in water. However, the releasing velocity was thereafter remarkably delayed, showing 75% after dissolution for 72hr.

리그닌의 應用 (第2報). 리그닌樹脂의 合成 및 接着力 調査

이갑용,박병각,이병근,Gab Yong Lee,Byung Kak Park,Byung Guen Lee 대한화학회 1976 대한화학회지 Vol.20 No.3

리그닌을 알칼리로 活性化시키고 페놀 및 포름알데히드와 反應시켜 새로운 樹脂를 合成하였다. 合成된 樹脂는 木材의 接着劑로 쓸 수 있는 충분한 强度를 가졌음을 확인하였다. A lignin resin, synthesized from the reaction of lignin, phenol and formaldehyde using NaOH as catalyst, showed a strong adhesive property. From a series adhesive strength test it has been shown that the synthetic resin can be used as a good adhesive material for wood.

산돌배나무(Pyrus ussuriensis)잎 추출물의 항산화활성에 관한 연구

이창언 ( Chang Eon Lee ),김영훈 ( Young Hun Kim ),이병근 ( Byung Guen Lee ),이도형 ( Do Hyung Lee ) 한국산림과학회 2010 한국산림과학회지 Vol.99 No.4

The aim of the study was to assess the cosmeceutical activity of Pyrus ussuriensis leaves and it is possible that can be used as a cosmetic ingredient for application of cosmetic industries. P t1sslirensis leaf was extracted with various solvents including water, 70% ethanol and 60% acetone. In the result of DPPH (J, 1diphenyl-2-picryl-hydrazyl) scavenging radical activity, water, ethanol and acetone extract of P. ussuriensis leaf were higher than 70%, 80% and 85`Vo at 50 ppm concentration, respectively. Xanthine oxidase inhibition activity and superoxide dismutase (SOD)-like activity by P ussuriensis extract were higher than 30%. In addition, SOD-like activity of all extracts showed tendency of the significant increase with the increase of concentration. In the anti-inflammatory test, P. ussuriensis leaf extract inhibited generation of nitric oxide (NO) stimulated by LPS in the macrophage cell line (raw :264.7) after 12 to 24 hours. As above results, P t1SSlIriensis has a great potential as a cosmeceutical raw material as well as anti-oxidant and anti-inflammatory ability.

산돌배나무(Pyrus ussuriensis) 잎 분획물의 항암 및 항균활성에 관한 연구

이창언 ( Chang Eon Lee ),김영훈 ( Young Hun Kim ),이병근 ( Byung Guen Lee ),이도형 ( Do Hyung Lee ) 한국임학회 2011 한국산림과학회지 Vol.100 No.2

본 연구는 산돌배나무 잎에서 추출한 분획물의 항암 및 항균효과를 조사하여 화장품소재로 적용 가능성을 확인하기 위하여 수행하였다. 산돌배나무 잎을 채취하여 건조시킨 후 분쇄하여 60% acetone-H2O(6:4, v/v)로 추출하고 아세톤 가용부를 분획하여 동결건조 시켰다. 아세톤 및 물 가용부를 Sephadex LH-20으로 충진한 칼럼에서 MeOH을 용출용매로 사용하여 칼럼크로마토그래피를 실시하고 또한 MeOH를 전개용매로 사용한 MIC-gel을 이용했다. 단리된 화합물들은 silica-gel TLC로 확인하였다. 항암효과는 마우스 피부암 세포(B16F10)를 이용한 MTT assay로 측정했다. 산돌배나무 잎으로부터 얻어진 분획물의 암세포 생장 저해율은 80% 이상의 저해률을 보였다. 항균활성 실험에서 산돌배나무 잎의 분획물은 그람양성균인 Staphylococcus aureus, Staphylococcus epidermidis에 대하여 0.25 mg/disc에서 1.3 cm, 2 cm의 생육 저해환을 나타내었다. 그람음성균인 Escherichia coli 에 대하여 0.5 mg/disc에서 각 분획물들은 1.1 cm~1.5 cm의 생육 저해환을 나타내었다. 이상의 결과로 미루어 보아 산돌배나무 잎의 아세톤 분획물은 항암 및 항균효과를 가진 천연소재임을 확인할 수 있었다. This study was conducted to confirm the application as ingredients of cosmetics through an examination of the function for anti-cancer and anti-microbial of the fraction isolated from Pyrus ussuriensis leaves. The dried leaf of P. ussuriensis were extracted with acetone-H2O (6:4, v/v), concentrated and fractionated with the upper layer of acetone on a separatory funnel. Each fraction was freeze dried, then a portion of acetone soluble powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol as eluents and also used the MIC-gel using a series of aqueous methanol as developing solvent. The isolated compounds were identified by silica-gel TLC. The growth inhibition activity was measured using the MTT assay by the mouse meltioma (B16F10) cell. The cancer cell growth inhibition rate of fractions isolated from P. ussuriensis leaf was 80%. In anti-microbial activity test, the fraction of P. ussuriensis with 0.25 mg/disc resulted in the clear zone of 1.3 cm and 2 cm for Staphylococcus aureus and S. epidermidis of gram positive bacillus, respectively. In Escherichia coli of gram negative bacillus, the fraction with 0.5 mg/disc resulted in the clear zone of 1.1 cm~1.5 cm each fraction. From these results, we confirmed that acetate fraction of P. ussuriensis has a great potential as a natural ingredients with a anti-cancer and anti-microbial source.

산돌배나무(Pyrus ussuriensis) 잎 분획물의 항산화 효과

이창언 ( Chang Eon Lee ),김영훈 ( Young Hun Kim ),이병근 ( Byung Guen Lee ),이도형 ( Do Hyung Lee ) 한국산림과학회 2010 한국산림과학회지 Vol.99 No.6

This study was conducted to confirm the application as ingredients of cosmetics through an examination of the function for anti-oxidant activity of the fraction isolated from Pyrus ussuriensis leaves. The dried leaf of Pyrus ussuriensis were extracted with acetone-H2O (6:4, v/v), concentrated and fractionated with the upper layer of acetone on a separatory funnel. Each fraction was freeze dried, then a portion of acetone soluble powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol as eluents and also used the MIC-gel using a series of aqueous methanol as developing solvent. The isolated compounds were identified by silica-gel TLC. The concentration of total phenolic compound of Pyrus ussuriensis acetate soluble fraction was high, 914 mg/g. The results obtained from the analyses of the anti-oxidanat effects of Pyrus ussuriensis acetate fraction can be summarized as follows: In the result of DPPH scavenging radical activity, Pyrus ussuriensis acetate soluble fraction showed more than 80% at 100 ppm. SOD-like activity of one of Pyrus ussuriensis acetate soluble fractions was 77% at 1000 ppm. Xanthine oxidase inhibition of Pyrus ussuriensis acetate soluble fraction was 38% at 100 ppm. From these results, we confirmed that acetate fraction of Pyrus ussuriensis has a great potential as a natural ingredients with a natural antioxidant and antimicrobial source.

리기다소나무(Pinus rigida Mill.) 내수피 추출물의 항산화효과에 관한 연구

장민정 ( Min Jung Jang ),안봉전 ( Bong Jeun An ),이창언 ( Chang Eeon Lee ),이진태 ( Jin Tae Lee ),이병근 ( Byung Guen Lee ),이도형 ( Do Hyung Lee ) 한국산림과학회 2008 한국산림과학회지 Vol.97 No.1

리기다소나무(Pinus rigida Mill.) 내수피 추출물에 대한 화장품 소재로써의 항염 및 항균효과

장민정 ( Min Jung Jang ),김영훈 ( Young Hun Kim ),안봉전 ( Bong Jeum An ),이창언 ( Chang Eeon Lee ),이진태 ( Jin Tae Lee ),김세현 ( Sea Hyun Kim ),이병근 ( Byung Guen Lee ),이도형 한국산림과학회 2008 한국산림과학회지 Vol.97 No.3

제지공장의 폐재인 Sludge 로 부터 합성 Board 의 제조

이병근 한국목재공학회 1987 목재공학 Vol.15 No.2

Fiber mats were made at various density levels, using fibers from papermill sludge, ricestraw and various mixtures of the two. The papermill sludges were collected from Moorim Papermill Co. and Jeonjoo Papermill Co. They were soaked in the liquid sulfur compounds, sulfur-tall oil and sulfurpolyster compounds, and made into fiber-reinforced, sulfur-based composition board. Under optimuan conditions of fiber that preparation and saturation with molten sulfur and modified sulfur, the Young's moduls of the manufactured fiber-reinforced composition board are superior to those of conventional wood-based composition boards. For example, the moduls of elasticity of the composition board made from papermill sludge, with a density of 0.40gm/㎤, were greater than 1,400,000psi as compared 800,000psi for high density hardboard(1.28gm/㎤). The modulus of rupture of the best reinforced composition board manufactured was over 9000psi, comparable to 6000psi of high density hardboard. The proposed Bryant and Lee's theory, $quot;Modified Rule of Mixtures$quot; can be applicable to the nonoriented and short fibrous composition board, when it was modified from $quot;Rule of Mixtures$quot; established by Paul an Jones, and supplemented by Smith and Cox's theory. In the Bryant and Lee's theory of Ec=⅓aE_fV_f+bE_mV_m, the constants $quot;a$quot; and $quot;b$quot; for the composition boards made from papermill sludge and the mixtures of ricestraw and the sludge were identified to be in the ranges of 3.29∼3.54 and -2.47∼2.80 respectively.

남자 초·중·고·대학생의 심폐기능에 관한 연구

전태원,이병근 師範大學 體育硏究所 1992 서울大學校 體育硏究所論集 Vol.13 No.1

The purpose of this study was to evaluate the differences between grade in the cardio-respiratory function variables of male elementary, middle, high school and college students. By open circuit method(Douglas bag method) 92 subjects (elementary, 24; high,24;college,20) performed maximal treadmill work with Bruce protocol to examine VO₂max and other the aerobic capacity variables. In statistical analysis, oneway-ANOVA was proceeded for comparing and analyzing the differences between grade of subjects. From the upper statistical procedure, the results were made as follows; 1. Maximal oxygen uptake (VO₂max) There was significant VO₂max difference between elementary, middle, high school and college students(1.446, 2.396, 2.887, 2.785ℓ/min; p<.01). 2. Maximal oxygen uptake in relation to body weight (VO₂max/㎏) There was no significant difference between elementary, middle, high school and college students (48.4, 45.8, 46.2, 44.4ml/㎏/min; p>.05) 3. Maximal oxygen uptake in relation to fat free weight (VO₂max/FFW) There was significant difference between elementary, middle and high school students (63.6, 60.7, 51.4ml/㎏/FFW; p<.01)> 4. Maximal ventilation (V?) There was significant difference between elementary, middle, high school and college students (57.7, 89.7, 108.3, 96.8ℓ/min;p<0.01). 5. maximal Oxygen ventilation equivalent (VEO₂) There was no significant difference between elementary, middle, high school and college students (40.3, 38.1, 37.7, 36.3; p=.15) 6. maximal Oxygen pulse (O₂pulse) There was significant difference between elementary, middle, high school and college students (7.6, 12.2, 14.4, 14.7ml/beat; p<.01). 7. maximal heart rate (max HR) There was significant difference between elementary, middle, high school and college students (195.5, 199.1, 200.4, 191.8 beats/min; p<.01).