http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
LC- MS/ MS를 이용한 마비성패류독소 분석조건 검토
송기철 ( Ki Cheol Song ),이가정 ( Ka Jeong Lee ),유홍식 ( Hong Sik Yu ),목종수 ( Jong Soo Mok ),김지회 ( Ji Hoe Kim ),임근식 ( Keun Sik Lim ),이미애 ( Mi Ac Lee ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.2
The AOAC Mouse Bioassay method (MBA) has been widely used for routine monitoring of paralytic shellfish poisoning (PSP) for more than 50 years. However, this method has low sensitivity and experiences interference from other components in the extract. Also, ethical issues have been raised against the continued use of this live-mouse assay. To establish an alternative method for PSP analysis, we attempted to develop PSP analysis conditions using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS analysis of reference material showed very reasonable accuracy, and the analysis time was just 15 mm. However, the recovery rate of toxin spike samples using the LC-MS/MS analysis was 59.4-91.0%. We also attempted to remove the matrix effect using shellfish extracts, but recoveries of C1 and C2 did not improve. A comparison between the results of MBA and LC-MS/MS analysis revealed good correlations, with values of 0.8878 and 0.9211 for oyster and mussel matrices, respectively.