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잠란(蠶卵)의 초기발육과정(初期發育過程)에 따르는 RNase활성(活性) 및 핵산량(核酸量)의 변동(變動) 및 그 X선조사(線照射)에 의한 영향(影響)에 관(關)한 연구(硏究)
이기영,전형원,Lee, K.Y.,Cheon, H.W. 한국응용생명화학회 1972 Applied Biological Chemistry (Appl Biol Chem) Vol.15 No.2
Previously identified female pupae were X-irradiated with a dose of 1000r one day prior to moth transformation. Female mothes from irradiated and non-irradiated pupae were copulated with normal male ones and allowed to lay eggs. Fertilized eggs were collected at 6 intervals such as 5, 15, 45, 90 minutes, 12 and 40 hours after laying, and deep-freezed immediately after each collection until measurements. RNase activity and nucleic acid content were determined with each sample and following results were obtained. 1) It was proved to exist two RNases in silk worm eggs as in mammalian tissues, one active maximally at pH 5.8 and the other at pH 8.0, and the acid RNase activity was much higher than that of alkaline RNase. 2) The activity of acid and alkaline RNases increased remarkably during early development of the embryo of silk worm eggs, reaching the maximum activity at 45 minutes from laying time in non-irradiated group. There was no appreciable difference in two RNase activities for 45 minutes after laying in both control and irradiated groups, but the activity of acid and alkaline RNases in latter group was three times as much as that in former group, at 90 minutes from laying time and it was also found the acid RNase activity was 1.8 times higher than alkaline one in irradiated group. 3) The RNA-P content of control group increased considerably for initial 45 minutes, followed by a decline 45 minutes later with sight but steady increase thereafter. The RNA-P content of irradiated group, however, increased at initial 5 minutes, followed by a marked fall 90 minutes after laying, with no change thereafter. The DNA-P of control group showed a sharp increase for initial 45 minutes, followed by a decline 45 minutes later with no appreciable change thereafter, whereas that of irradiated group showed an increase at initial 15 minutes, followed by a sharp decline for following 45 minutes with a gradual increase thereafter. It was thus proved that the synthesis of nucleic acid in silk worm eggs was much suppressed by X-irradiation during early development of embryo. 4) The RNase activity varied in parallel with the RNA-P content in control group, but the RNA-P content in irradiated group was shown to be minimum value in concidence with the maximum activity of both RNases.
Ni-Cr-(Ti,Nb,Fe)계 초내열합금 제조 및 평가
이기영(K. Y. Lee),안장홍(J. H. Ahn),손희영(H. Y. Son),남희수(H.S. Nam),김동배(D. B. Kim),이락규(L. G Lee),이기대(K. D. Lee) 한국소성가공학회 2011 한국소성가공학회 학술대회 논문집 Vol.2011 No.5
Nickel based Ni-Cr-(Ti,Nb,Fe)Super-alloy has been used in gas turbine and valve spring because of its low thermal expansion ratio, corrosion resistance, and high creep strength properties. Likewise other super-alloys composed of Ni-Cr-(Ti,Nb,Fe) have been imported to advance domestic manufacturing technology in the field of ingot making and ingot’s break down. In this project, the overall manufacturing process has been successfully performed, and the appraisal on nickel based Ni-Cr-(Ti,Nb,Fe)Super-alloy forged bar has been also carried out in the analysis of microstructure and mechanical properties with heat treatment.